Team:Freiburg bioware

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<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" dir="ltr" lang="en-US"> <head> <meta http-equiv="Content-Type" content="text/html; charset=UTF-8" /> <meta http-equiv="X-UA-Compatible" content="IE=EmulateIE7" /> <title>Artisteer</title> <script type="text/javascript" src="script.js"></script> <link rel="stylesheet" href="/wiki/index.php?title=User:Maximi/style_home.css&action=raw&ctype=text/css" type="text/css" media="screen" />  </head> <body> <div id="art-page-background-simple-gradient"> </div> <div id="art-main"> <div class="art-Sheet"> <div class="art-Sheet-tl"></div> <div class="art-Sheet-tr"></div> <div class="art-Sheet-bl"></div> <div class="art-Sheet-br"></div> <div class="art-Sheet-tc"></div> <div class="art-Sheet-bc"></div> <div class="art-Sheet-cl"></div> <div class="art-Sheet-cr"></div> <div class="art-Sheet-cc"></div> <div class="art-Sheet-body"> <div class="art-Header"> <div class="art-Header-jpeg"></div> </div> <div class="art-nav"> <div class="l"></div> <div class="r"></div> <ul class="art-menu"> <li><a href="https://2009.igem.org/Team:Freiburg_bioware" class="active"><span class="l"></span><span class="r"></span><span class="t">Home</span></a></li> <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Team"><span class="l"></span><span class="r"></span><span class="t">The Team</span></a> <ul> <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Team">Overview</a></li> <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Team/Portraits">Portraits</a></li> </ul> </li> <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Project"><span class="l"></span><span class="r"></span><span class="t">The Project</span></a> <ul> <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Project#Summary">Summary</a> </li> <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Project#Subprojects">Subprojects</a></li> <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Project#Highlights">Highlights</a></li> </ul> </li> <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Human_Practice"><span class="l"></span><span class="r"></span><span class="t">Human Practice</span></a> <ul> <li><a href="#">1</a> <ul> <li><a href="#">2</a> </li> <li><a href="#">3</a> </li> <li><a href="#">4</a> </li> </ul> </li> <li><a href="#">5</a></li> <li><a href="#">6</a></li> </ul> </li> <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Notebook"><span class="l"></span><span class="r"></span><span class="t">Notebook</span></a></li> <li><a href="https://2009.igem.org/Team:Freiburg_bioware/cloning"><span class="l"></span><span class="r"></span><span class="t">Parts</span></a> <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Collaboration"><span class="l"></span><span class="r"></span><span class="t">Collaboration</span></a> </ul> </div> <div class="art-contentLayout"> <div class="art-content"> <div class="art-Post"> <div class="art-Post-tl"></div> <div class="art-Post-tr"></div> <div class="art-Post-bl"></div> <div class="art-Post-br"></div> <div class="art-Post-tc"></div> <div class="art-Post-bc"></div> <div class="art-Post-cl"></div> <div class="art-Post-cr"></div> <div class="art-Post-cc"></div> <div class="art-Post-body"> <div class="art-Post-inner"> <div class="art-PostMetadataHeader"> <h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> <span class="art-PostHeader">Welcome</span> </h2> </div> <div class="art-PostContent"> <img class="art-article" src="https://static.igem.org/mediawiki/2009/9/9e/Freiburg09_Kuklab_logo.jpg" style="width: 205px; height: 205px; float: left;" /> <h1 style="border-bottom: none;">Team Freiburg Bioware</h1> <br /> <p>The first German team ever to participate in iGEM is back again and after last year's second place we're highly motivated to make some good piece of synthetic biology in 2009. In this year we want to create an universal restriction enzyme to facilitate labwork and enable new techniques. <br /> <br /> We're looking forward to meeting you on this year's jamboree!<br /> </p> <p> <span class="art-button-wrapper"> <span class="r"></span> </span> </p> <br /> </div> <div class="cleared"></div> </div> </div> </div> <div class="art-Post"> <div class="art-Post-tl"></div> <div class="art-Post-tr"></div> <div class="art-Post-bl"></div> <div class="art-Post-br"></div> <div class="art-Post-tc"></div> <div class="art-Post-bc"></div> <div class="art-Post-cl"></div> <div class="art-Post-cr"></div> <div class="art-Post-cc"></div> <div class="art-Post-body"> <div class="art-Post-inner"> <div class="art-PostMetadataHeader"> <h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> <span class="art-PostHeader">Project Summary</span> </h2> </div> <div class="art-PostContent"><span class="art-button-wrapper"></span><b><br /> Universal Endonuclease – Cutting Edge Technology</b> <div class="art-PostContent"> <p>Gene technology is driven by the use of restriction endonucleases. Yet, constraints of limited sequence length and variation recognized by available restriction enzymes pose a major roadblock for synthetic biology. We developed the basis for universal restriction enzymes, primarily for routine cloning but also with potential for in vivo applications. We use a nucleotide cleavage domain fused to a binding domain, which recognizes a programmable adapter that mediates binding to DNA and thus cleavage. As adapter we use readily available modified oligonucleotides, as binding domain anticalins and as cleavage domain FokI moieties engineered for heterodimerization and activity. For cloning, this universal enzyme has merely to be mixed with the sequence specific oligonucleotide and the target DNA. Binding and release are addressed with thermocycling. Additionally, we provide concepts for in vivo applications by external adapter delivery, activity regulation by photo-switching, as well as for modifying an argonaute protein towards a DNA endonuclease. </p> </div> <p></p> </div> <div class="cleared"></div> </div> </div> </div> <div class="art-Post"> <div class="art-Post-tl"></div> <div class="art-Post-tr"></div> <div class="art-Post-bl"></div> <div class="art-Post-br"></div> <div class="art-Post-tc"></div> <div class="art-Post-bc"></div> <div class="art-Post-cl"></div> <div class="art-Post-cr"></div> <div class="art-Post-cc"></div> <div class="art-Post-body"> <div class="art-Post-inner"> <div class="art-PostMetadataHeader"> <h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> <span class="art-PostHeader">Sponsors</span> </h2> </div> <div class="art-PostContent"><span class="art-button-wrapper"></span><b><br /> <img style="width: 730px; height: 300px;" alt="Sponsors" src="https://static.igem.org/mediawiki/2009/b/b8/Freiburg09_Sponsors.png" /><br /> </b> <p></p> </div> <div class="cleared"></div> </div> </div> </div> </div> <div class="art-sidebar1"> <div class="art-Block"> <div class="art-Block-tl"></div> <div class="art-Block-tr"></div> <div class="art-Block-bl"></div> <div class="art-Block-br"></div> <div class="art-Block-tc"></div> <div class="art-Block-bc"></div> <div class="art-Block-cl"></div> <div class="art-Block-cr"></div> <div class="art-Block-cc"></div> <div class="art-Block-body"> <div class="art-BlockHeader"> <div class="l"></div> <div class="r"></div> <div class="art-header-tag-icon"> <div class="t">FREiGEM 2009</div> </div> </div> <div class="art-BlockContent"> <div class="art-BlockContent-body"> <div> <p><a href="https://2009.igem.org/Team:Freiburg_bioware/Team"><span style="font-weight: bold;"><img style="border: 0px solid ; width: 138px; height: 40px;" alt="Team" src="https://static.igem.org/mediawiki/2009/f/f6/Freiburg09_Team.gif" /></span></a><br /> <b>The Team</b><br /> In 2009 our team consists of 14 undergraduates and 4 advisors.<br /> <a href="https://2009.igem.org/Team:Freiburg_bioware/Team">Read more...</a></p> <p><span style="font-weight: bold;"><a href="http://www.bioss.uni-freiburg.de"><img style="border: 0px solid ; width: 144px; height: 48px;" alt="bioss" src="https://static.igem.org/mediawiki/2009/3/34/Freiburg09_Bioss_portlet.jpg" /></a><br /> Bioss</span><br /> We want to thank our main sponsor Bioss for supporting our project.<br /> <a href="http://www.bioss.uni-freiburg.de">Read more...</a></p> </div> </div> </div> </div> </div> <div class="art-Block"> <div class="art-Block-tl"></div> <div class="art-Block-tr"></div> <div class="art-Block-bl"></div> <div class="art-Block-br"></div> <div class="art-Block-tc"></div> <div class="art-Block-bc"></div> <div class="art-Block-cl"></div> <div class="art-Block-cr"></div> <div class="art-Block-cc"></div> <div class="art-Block-body"> <div class="art-BlockHeader"> <div class="l"></div> <div class="r"></div> <div class="art-header-tag-icon"> <div class="t">Visitors</div> </div> </div> <div class="art-BlockContent"> <div class="art-BlockContent-body"> <div> <br /> <a href="http://www3.clustrmaps.com/counter/maps.php?url=https://2009.igem.org/Team:Freiburg_bioware" id="clustrMapsLink"><img src="http://www3.clustrmaps.com/counter/index2.php?url=https://2009.igem.org/Team:Freiburg_bioware" style="border: 0px none ; width: 144px; height: 95px;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://www2.clustrmaps.com';" /> </a><br /> </div> </div> </div> </div> </div> </div> </div> <div class="cleared"></div> <div class="art-Footer"> <div class="art-Footer-inner"> <div class="art-Footer-text"> <p><a href="mailto:freigem09@googlemail.com"> <span style="text-decoration: underline;">freigem09@googlemail.com</span></a></p> </div> </div> <div class="art-Footer-background"></div> </div> </div> </div> <div class="cleared"></div> <p class="art-page-footer"> </div> </body> </html>