Team:Freiburg bioware/Notebook/April

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Revision as of 10:19, 20 October 2009

FREiGEM

  • Notebook
  • Parts
  • Collaboration

    •   April

    24th April 12:30 - 18:00 o'clock Manuel

    Literaturrecherche zu FokI.
    Entstandene Ideen:
    • three oligo's: two for binding the monomers and one to create a dsDNA where FokI can bind.
    • two oligo's: one for binding one monomer, the second for binding the second monomer and to create dsDNA (one monomer has to be inactivated).
    • one oligo: both binding sites of the monomers on one oligo (also one monomer has to be inactivated).

    The 196 C-terminale aminoacids of FokI were used from other groups (see reference 1). Primersequences are also provided in the publication.
    Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf

    29th april 21o'clock Rüdiger


    contact:  freigem09@googlemail.com

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