Team:Freiburg bioware/cloning

From 2009.igem.org

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<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
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<div class="art-PostContent">
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<div style="text-align: center;">
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</div><a name="4"></a>
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<table border="5">
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<td align="center">
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<img src="https://static.igem.org/mediawiki/2009/thumb/f/fa/Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.jpg" name="Strep+FluA+Split+Foki in pMA Vector" width="480" height="480" />
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<br><p align="center">Strep+FluA+Split+Foki in pMA Vector</p>
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</td>
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<td align="left">
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nucleotid sequence of ORF-1:<br>
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GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
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CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
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ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
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GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
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GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
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CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
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GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
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CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
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GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
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GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
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CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
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ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA
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GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT
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GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA
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TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG
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TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG
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TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG
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TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT
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CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA
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ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG
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AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC
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CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG
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TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA
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CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC
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GGTTAA
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</td>
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</tr>
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</table>
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<br>
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<h4>AA sequence of ORF-1:<br></h4>
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<table border="0">
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<p align="justify"><td align="left">
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GWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
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EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
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IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
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CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ
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DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG
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QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN
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SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
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<br><br></p></td></table>
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<h4>Commented GenBank file</h4>
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<li> <a href="https://static.igem.org/mediawiki/2009/8/8d/Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.txt">Strep+FluA+Split+Foki_pMA.txt</a></li>
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Revision as of 17:48, 13 October 2009

FREiGEM

overview


His+FluA+Split+Foki_pMA
His+Dig+Split+FokA_pMA
DsbA+His+Dig+Split+FokA_pMA

 Parts

 His+FluA+Split+Foki_pMA


His+FluA+SplitLinker+Foki in pMA Vector

 nucleotid sequence of ORF-1:
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC GGTTAA

AA sequence of ORF-1:

MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ
DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG
QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN
SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

Commented GenBank file

  • His+FluA+Split+Foki_pMA.txt



    •  His+Dig+Split+FokA_pMA


    His+Dig+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTT ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCC TATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCG TTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAAT GGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTG AGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCA TAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTG GCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGT AAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKA YSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA QLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+Split+FokA_pMA.txt



    •  DsbA+His+Dig+Split+FokA_pMA


    DsbA+His+Dig+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC ATCGGCGACCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACG ACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAG TACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAA GTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGA AGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGC ACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTA CACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCG ACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAG AAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGAC CGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGG TGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAA GTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACC AGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCG GCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAA TATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTAC CCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCT ATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCC ATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAA AGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGC AGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAAC GAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTT CGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGA ACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTG ATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCG CCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MKKIWLALAGLVLAFSASATGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAA YPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIG GVTQVGVSNVLSTDNKNYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIG SPVVDSQKLVYSDFSEAACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEE KKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGA IYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSV TEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKF NNGEINFTG

    Commented GenBank file

  • DsbA+His+Dig+Split+FokA_pMA.txt



    •  Strep+FluA+ShortLinker+FokI_pMA


    Strep+FluA+ShortLinker+FokI in pMA Vector

    		 nucleotid sequence of ORF-1:
    GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAA AAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGA ACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGA AAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTG GGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGAT CGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATC TGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAG ACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTC AAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCA ACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGG GCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGC AGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGA TCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    GWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFM KVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQ TRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLI GGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+ShortLinker+FokI_pMA.txt



    •  His+FluA+middellinker+FokA_pMA


    His+FluA+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT CTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCC GATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATA ACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAAC CAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCC GTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAG GCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAAT GGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAA AGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGG AGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF MKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKEN QTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELL IGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+middellinker+FokA_pMA.txt



    •  His+FluA+middellinker+Foki_pMA


    His+FluA+middellinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT CTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCC GATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATA ATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAAT CAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCC GTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAG GCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAAT GGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAA AGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCG AGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF MKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEEN QTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELL IGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+middellinker+Foki_pMA.txt



    •  His+FluA+shortlinker+FokA_pMA


    His+FluA+shortlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC CGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG CGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTG GTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAAC AAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTAC AGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAG CACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTG TCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCT GACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTA CCGGTTAA

    AA sequence of ORF-1:

    HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY GYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRN KHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGE MIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+shortlinker+FokA_pMA.txt



    •  His+FluA+shortlinker+Foki_pMA


    His+FluA+shortlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC CGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG CGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTG GTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAAC AAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCAC CGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAG CCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTG AGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCT GACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTA CCGGTTAA

    AA sequence of ORF-1:

    HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY GYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRN KHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGE MIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+shortlinker+Foki_pMA.txt



    •  His+Dig+longlinker+FokA_pMA


    His+Dig+longlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT TTTACCGGTTAA

    AA sequence of ORF-1:

    HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+longlinker+FokA_pMA.txt



    •  His+Dig+longlinker+Foki_pMA


    His+Dig+longlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG TAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATT ATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCG ATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCG TAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTG TCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTAT AAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGT GCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCA CCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAAT TTTACCGGTTAA

    AA sequence of ORF-1:

    HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLP IGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHI TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+longlinker+Foki_pMA.txt



    •  Strep+FluA+Split+Foki_pMA


    Strep+FluA+Split+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC GGTTAA

    AA sequence of ORF-1:

    GWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+Split+Foki_pMA.txt





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    Retrieved from "http://2009.igem.org/Team:Freiburg_bioware/cloning"