Team:Freiburg bioware/cloning1

From 2009.igem.org

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  class="t">The Team</span></a>
  class="t">The Team</span></a>
     <ul>
     <ul>
-
       <li><a href="#">Overview</a></li>
+
       <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Team">Overview</a></li>
-
       <li><a href="#">Photos</a></li>
+
       <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Team/Portraits">Portraits</a></li>
     </ul>
     </ul>
   </li>
   </li>
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  href="https://2009.igem.org/Team:Freiburg_bioware/Project#Summary">Summary</a>
  href="https://2009.igem.org/Team:Freiburg_bioware/Project#Summary">Summary</a>
       </li>
       </li>
-
       <li><a
+
        
-
href="https://2009.igem.org/Team:Freiburg_bioware/Project#Subprojects">Subprojects</a></li>
+
       <li><a
       <li><a
  href="https://2009.igem.org/Team:Freiburg_bioware/Project#Highlights">Highlights</a></li>
  href="https://2009.igem.org/Team:Freiburg_bioware/Project#Highlights">Highlights</a></li>
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  class="t">Human
  class="t">Human
Practice</span></a>
Practice</span></a>
-
    <ul>
 
-
      <li><a href="#">D'oh! Something's missing here.</a>
 
         <ul>
         <ul>
-
          <li><a href="#">1</a> </li>
+
      <li><a
-
          <li><a href="#">2</a> </li>
+
href="https://2009.igem.org/Team:Freiburg_bioware/Human_Practice/Ethics">Ethics</a>
-
          <li><a href="#">3</a> </li>
+
-
        </ul>
+
       </li>
       </li>
-
       <li><a href="#">4</a></li>
+
       <li><a
-
      <li><a href="#">5</a></li>
+
href="https://2009.igem.org/Team:Freiburg_bioware/Human_Practice/Safety">Safety</a></li>
     </ul>
     </ul>
 +
   </li>
   </li>
   <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Notebook"><span class="l"></span><span
   <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Notebook"><span class="l"></span><span
  class="r"></span><span class="t">Notebook</span></a></li>
  class="r"></span><span class="t">Notebook</span></a></li>
   <li><a
   <li><a
-
  href="https://2009.igem.org/Team:Freiburg_bioware/cloning" class="active"><span
+
  href="https://2009.igem.org/Team:Freiburg_bioware/cloning1"class="active"><span
  class="l"></span><span class="r"></span><span
  class="l"></span><span class="r"></span><span
-
  class="t">Parts</span></a></li>
+
  class="t">Parts</span></a><ul>
 +
      <li><a
 +
href="https://2009.igem.org/Team:Freiburg_bioware/cloning1">Basic
 +
Parts</a></li>
 +
      <li><a
 +
href="https://2009.igem.org/Team:Freiburg_bioware/cloning">Composite
 +
Parts</a></li>
 +
    </ul></li>
<li><a
<li><a
  href="https://2009.igem.org/Team:Freiburg_bioware/Collaboration"><span
  href="https://2009.igem.org/Team:Freiburg_bioware/Collaboration"><span
  class="l"></span><span class="r"></span><span
  class="l"></span><span class="r"></span><span
  class="t">Collaboration</span></a></li>
  class="t">Collaboration</span></a></li>
 +
<li><a
 +
href="https://2009.igem.org/Team:Freiburg_bioware/Modeling"><span
 +
class="l"></span><span class="r"></span><span
 +
class="t">Modeling</span></a></li>
</ul>
</ul>
</div>
</div>
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<div class="art-Post-inner">
<div class="art-Post-inner">
<div class="art-PostMetadataHeader">
<div class="art-PostMetadataHeader">
-
 
+
<a name="1"></a>
-
 
+
<h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> &nbsp;Index<span
-
<p></div><a name="1"></a>
+
class="art-PostHeader"></span> </h2>
-
<h3>Index</h3><br>
+
</div>
-
 
+
<div class="art-PostContent">
 +
<div style="text-align: left;">
<table border="0">
<table border="0">
<tr><td>
<tr><td>
<p><font size="2">
<p><font size="2">
-
*<a href="#2">His+FluA+Split+Foki_pMA
+
*<a href="#2">FokA_pMA
</a><br>
</a><br>
-
*<a href="#3">His+Dig+Split+FokA_pMA
+
*<a href="#3">Foki_pMA
</a><br>
</a><br>
-
*<a href="#4">
+
*<a href="#4">Dig_pMA
-
DsbA+His+Dig+Split+FokA_pMA
+
</a><br>
</a><br>
-
*<a href="#5">
+
*<a href="#5">FluA_pMA
-
Strep+FluA+ShortLinker+FokI_pMA
+
</a><br>
</a><br>
-
*<a href="#6">
+
*<a href="#6">DsbA_pMA
-
His+FluA+middellinker+FokA_pMA
+
</a><br>
</a><br>
-
*<a href="#7">
+
*<a href="#7">StrepTag_pMA
-
His+FluA+middellinker+Foki_pMA
+
</a><br>
</a><br>
-
*<a href="#8">
+
*<a href="#8">HisTag_pMA
-
His+FluA+shortlinker+FokA_pMA
+
</a><br>
</a><br>
-
*<a href="#9">
+
*<a href="#9">shortlinker26_pMA
-
His+FluA+shortlinker+Foki_pMA
+
</a><br>
</a><br>
-
*<a href="#10">
+
*<a href="#10">Middellinker38_pMA
-
His+Dig+longlinker+FokA_pMA
+
</a><br>
</a><br>
-
*<a href="#11">
+
*<a href="#11">LongLinkerA50_pMA
-
His+Dig+longlinker+Foki_pMA
+
</a><br>
</a><br>
-
*<a href="#12">
+
*<a href="#12">GSAT_linker_pMA
-
Strep+FluA+Split+Foki_pMA
+
</a><br>
</a><br>
-
*<a href="#13">
+
*<a href="#13">SEG_Linker_pMA  
-
  His+Dig+middellinker+FokA_pMA
+
</a><br>
</a><br>
-
*<a href="#14">
+
*<a href="#14">Split_Linker_pMA
-
His+Dig+middellinker+Foki_pMA
+
</a><br>
</a><br>
-
*<a href="#15">
+
*<a href="#15">YFP(Venus)_pGA14
-
His+Dig+shortlinker+FokA_pMA
+
</a><br>
</a><br>
-
*<a href="#16">
+
*<a href="#19">Fos_pMA
-
His+Dig+shortlinker+Foki_pMA
+
</a><br>
</a><br>
-
*<a href="#17">
+
*<a href="#16">pJS418 Vector
-
His+Dig+Split+Foki_pMA
+
</a><br>
</a><br>
-
*<a href="#18">
+
*<a href="#17">pJS419 Vector
-
His+FluA+longlinker+FokA_pMA
+
</a><br>
</a><br>
-
</p></font></p>
+
*<a href="#18">pBAD Vector
-
 
+
-
</td>
+
-
<td>
+
-
<p><font size="2">
+
-
*<a href="#19">
+
-
His+FluA+longlinker+Foki_pMA
+
</a><br>
</a><br>
-
*<a href="#20">
+
*<a href="#20">pEX Vector
-
His+FluA+Split+FokA_pMA
+
-
</a><br>
+
-
*<a href="#21">
+
-
Strep+Dig+longlinker+FokA_pMA
+
-
</a><br>
+
-
*<a href="#22">
+
-
Strep+Dig+longlinker+Foki_pMA
+
-
</a><br>
+
-
*<a href="#23">
+
-
Strep+Dig+middellinker+FokA_pMA
+
-
</a><br>
+
-
*<a href="#24">
+
-
Strep+Dig+middellinker+Foki_pMA
+
-
</a><br>
+
-
*<a href="#25">
+
-
Strep+FluA+Split+FokA_pMA
+
-
</a><br>
+
-
*<a href="#26">
+
-
Strep+FluA+Split+FokA_pMA
+
-
</a><br>
+
-
*<a href="#27">
+
-
Strep+Dig+shortlinker+FokA_pMA
+
-
</a><br>
+
-
*<a href="#28">
+
-
Strep+Dig+shortlinker+Foki_pMA
+
-
</a><br>
+
-
*<a href="#29">
+
-
Strep+Dig+Split+FokA_pMA
+
-
</a><br>
+
-
*<a href="#30">
+
-
Strep+Dig+Split+Foki_pMA
+
-
</a><br>
+
-
*<a href="#31">
+
-
Strep+FluA+longlinker+FokA_pMA
+
-
</a><br>
+
-
*<a href="#32">
+
-
Strep+FluA+longlinker+Foki_pMA
+
-
</a><br>
+
-
*<a href="#33">
+
-
Strep+FluA+middellinker+FokA_pMA
+
-
</a><br>
+
-
*<a href="#34">
+
-
Strep+FluA+middellinker+Foki_pMA
+
-
</a><br>
+
-
*<a href="#35">
+
-
Strep+FluA+shortlinker+FokA_pMA
+
</a><br>
</a><br>
</p></font></p>
</p></font></p>
-
 
-
 
</td></tr></table>
</td></tr></table>
 +
<li style=" text-align:center;"><a href="https://2009.igem.org/Team:Freiburg_bioware/oligos">list of oligonucleotides</a></li>
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-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
<h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> &nbsp;Parts<span class="art-PostHeader"></span> </h2>
 
</div>
</div>
-
 
+
<br />
-
 
+
<div style="text-align: center;">
-
</div><a name="2"></a>
+
</div>
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
+
<br />
-
<div class="art-PostContent">
+
</div>
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
+
<div class="cleared"></div>
-
 
+
-
 
+
-
<table border="5">
+
-
<tr>
+
-
<td align="center">
+
-
<img src="https://static.igem.org/mediawiki/2009/3/37/Freiburg09_His%2BFluA%2BSplit%2BFokI_pMA.jpg" name="His+FluA+SplitLinker+Foki in pMA Vector" width="480" height="480" />
+
-
<br><p align="center">His+FluA+SplitLinker+Foki in pMA Vector</p>
+
-
</td>
+
-
<td align="left">
+
-
nucleotid sequence of ORF-1:<br>
+
-
 
+
-
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG
+
-
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
+
-
ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
+
-
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
+
-
GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
+
-
CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
+
-
GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
+
-
CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
+
-
GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
+
-
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
+
-
CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
+
-
ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA
+
-
GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT
+
-
GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA
+
-
TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG
+
-
TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG
+
-
TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG
+
-
TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT
+
-
CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA
+
-
ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG
+
-
AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC
+
-
CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG
+
-
TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA
+
-
CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC
+
-
GGTTAA
+
-
</td>
+
-
</tr>
+
-
</table>
+
-
<br>
+
-
<h4>AA sequence of ORF-1:<br></h4>
+
-
<table border="0">
+
-
<p align="justify"><td align="left">
+
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP<br>
+
-
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY<br>
+
-
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA<br>
+
-
CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ<br>
+
-
DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG<br>
+
-
QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN<br>
+
-
SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG<br><br></p></td></table>
+
-
 
+
-
<h4>Commented GenBank file</h4>
+
-
<li> <a href="https://static.igem.org/mediawiki/2009/d/d8/Freiburg09_His%2BFluA%2BSplit%2BFokI_pMA.txt">His+FluA+Split+Foki_pMA.txt</a></li>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
</div><a name="3"></a>
+
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
+
-
 
+
-
<div class="art-PostContent">
+
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
+
-
 
+
-
<table border="5">
+
-
<tr>
+
-
<td align="center">
+
-
<img src="https://static.igem.org/mediawiki/2009/3/34/Freiburg09_His%2BDig%2BSplit%2BFokA_pMA.JPG" name="His+Dig+Split+FokA in pMA Vector" width="480" height="480" />
+
-
<br><p align="center">His+Dig+Split+FokA in pMA Vector</p>
+
-
</td>
+
-
<td align="left">
+
-
nucleotid sequence of ORF-1:<br>
+
-
 
+
-
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG
+
-
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
+
-
ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
+
-
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
+
-
GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
+
-
CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
+
-
ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
+
-
CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
+
-
GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
+
-
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
+
-
CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
+
-
ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC
+
-
TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA
+
-
ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG
+
-
TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA
+
-
GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG
+
-
CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTT
+
-
ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCC
+
-
TATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCG
+
-
TTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAAT
+
-
GGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTG
+
-
AGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCA
+
-
TAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTG
+
-
GCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGT
+
-
AAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
+
-
 
+
-
</td>
+
-
</tr>
+
-
</table>
+
-
<br>
+
-
<h4>AA sequence of ORF-1:<br></h4>
+
-
<table border="0">
+
-
<p align="justify"><td align="left">
+
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
+
-
EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
+
-
IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
+
-
CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE
+
-
LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKA
+
-
YSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA
+
-
QLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
-
<br><br></p></td></table>
+
-
 
+
-
<h4>Commented GenBank file</h4>
+
-
<li> <a href="https://static.igem.org/mediawiki/2009/d/dd/Freiburg09_His%2BDig%2BSplit%2BFokA_pMA.txt">His+Dig+Split+FokA_pMA.txt</a></li>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
</div><a name="4"></a>
+
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;DsbA+His+Dig+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
+
</div>
</div>
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/4/4f/Freiburg09_DsbA%2BHis%2BDig%2BSplit%2BFokA_pMA.JPG" name="DsbA+His+Dig+Split+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">DsbA+His+Dig+Split+FokA in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC
 
-
ATCGGCGACCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACG
 
-
ACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAG
 
-
TACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAA
 
-
GTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGA
 
-
AGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGC
 
-
ACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTA
 
-
CACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCG
 
-
ACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAG
 
-
AAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGAC
 
-
CGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGG
 
-
TGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAA
 
-
GTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACC
 
-
AGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCG
 
-
GCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAA
 
-
TATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTAC
 
-
CCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCT
 
-
ATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCC
 
-
ATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAA
 
-
AGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGC
 
-
AGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAAC
 
-
GAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTT
 
-
CGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGA
 
-
ACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTG
 
-
ATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCG
 
-
CCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGMKKIWLALAGLVLAFSASATGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAA
 
-
YPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIG
 
-
GVTQVGVSNVLSTDNKNYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIG
 
-
SPVVDSQKLVYSDFSEAACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEE
 
-
KKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGA
 
-
IYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSV
 
-
TEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKF
 
-
NNGEINFTG
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/4/4d/Freiburg09_DsbA%2BHis%2BDig%2BSplit%2BFokA_pMA.txt">DsbA+His+Dig+Split+FokA_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
</div><a name="5"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+ShortLinker+FokI_pMA<span class="art-PostHeader"></span> </h3>
 
</div>
</div>
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/0/03/Freiburg09_Strep%2BFluA%2BShortLinker%2BFokI_pMA.jpg" name="Strep+FluA+ShortLinker+FokI in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+FluA+ShortLinker+FokI in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCC
 
-
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
 
-
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 
-
ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
 
-
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 
-
GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
 
-
CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
 
-
GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
 
-
CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
 
-
GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 
-
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 
-
CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
 
-
ACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAA
 
-
AAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGA
 
-
ACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGA
 
-
AAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTG
 
-
GGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGAT
 
-
CGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATC
 
-
TGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAG
 
-
ACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTC
 
-
AAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCA
 
-
ACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGG
 
-
GCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGC
 
-
AGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGA
 
-
TCAATTTTACCGGTTAA
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
 
-
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
 
-
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
 
-
CKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFM
 
-
KVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQ
 
-
TRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLI
 
-
GGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
<br><br></p></td></table>
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/7/7e/Freiburg09_Strep%2BFluA%2BShortLinker%2BFokI_pMA.txt">Strep+FluA+ShortLinker+FokI_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
</div><a name="6"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
</div>
</div>
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/a/ad/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.jpg" name="His+FluA+middellinker+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">His+FluA+middellinker+FokA in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
ATGGCCGGC
+
<div class="art-contentLayout">
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
<div class="art-content">
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
+
<div class="art-Post">
-
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
+
<div class="art-Post-tl"></div>
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
+
<div class="art-Post-tr"></div>
-
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
+
<div class="art-Post-bl"></div>
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
+
<div class="art-Post-br"></div>
-
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
+
<div class="art-Post-tc"></div>
-
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
+
<div class="art-Post-bc"></div>
-
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
+
<div class="art-Post-cl"></div>
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
+
<div class="art-Post-cr"></div>
-
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
+
<div class="art-Post-cc"></div>
-
GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG
+
<div class="art-Post-body">
-
AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT
+
<div class="art-Post-inner">
-
CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA
+
<div class="art-PostMetadataHeader">
-
TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT
+
-
CTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCC
+
-
GATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATA
+
-
ACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAAC
+
-
CAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCC
+
-
GTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAG
+
-
GCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAAT
+
-
GGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAA
+
-
AGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGG
+
-
AGATCAATTTTACCGGTTAA
+
-
 
+
<h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> &nbsp;Parts<span
-
</td>
+
class="art-PostHeader"></span> </h2>
-
</tr>
+
-
</table>
+
-
<br>
+
-
<h4>AA sequence of ORF-1:<br></h4>
+
-
<table border="0">
+
-
<p align="justify"><td align="left">
+
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
+
-
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
+
-
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
+
-
NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF
+
-
MKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKEN
+
-
QTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELL
+
-
IGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
-
 
+
-
 
+
-
<br><br></p></td></table>
+
-
 
+
-
<h4>Commented GenBank file</h4>
+
-
<li> <a href="https://static.igem.org/mediawiki/2009/7/78/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.txt">His+FluA+middellinker+FokA_pMA.txt</a></li>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
 
+
-
</div><a name="7"></a>
+
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
+
</div>
</div>
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/c/ce/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.jpg" name="His+FluA+middellinker+Foki in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">His+FluA+middellinker+Foki in pMA Vector</p><br>
 
-
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243014">link to part registry</a>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGC
 
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 
-
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
 
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
 
-
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
 
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
 
-
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
 
-
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
 
-
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 
-
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
 
-
GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG
 
-
AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT
 
-
CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA
 
-
TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT
 
-
CTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCC
 
-
GATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATA
 
-
ATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAAT
 
-
CAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCC
 
-
GTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAG
 
-
GCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAAT
 
-
GGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAA
 
-
AGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCG
 
-
AGATCAATTTTACCGGTTAA
 
-
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
 
-
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
 
-
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 
-
NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF
 
-
MKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEEN
 
-
QTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELL
 
-
IGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/9/92/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.txt">His+FluA+middellinker+Foki_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
</div><a name="8"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
<div class="art-PostContent">
<div class="art-PostContent">
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
+
<div style="text-align: center;">
-
<table border="5">
+
<a name="2"></a>
-
<tr>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;FokA_pMA<span class="art-PostHeader"></span> </h3>
-
<td align="center">
+
-
<img src="https://static.igem.org/mediawiki/2009/thumb/d/d4/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.jpg" name="His+FluA+shortlinker+FokA in pMA Vector" width="480" height="480" />
+
-
<br><p align="center">His+FluA+shortlinker+FokA in pMA Vector</p>
+
-
</td>
+
-
<td align="left">
+
-
nucleotid sequence of ORF-1:<br>
+
-
 
+
-
ATGGCCGGC
+
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
+
-
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
+
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
+
-
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
+
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
+
-
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
+
-
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
+
-
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
+
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
+
-
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
+
-
GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG
+
-
CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA
+
-
GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG
+
-
AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC
+
-
CGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG
+
-
CGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTG
+
-
GTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAAC
+
-
AAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTAC
+
-
AGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAG
+
-
CACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTG
+
-
TCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCT
+
-
GACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTA
+
-
CCGGTTAA
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
</td>
+
-
</tr>
+
-
</table>
+
-
<br>
+
-
<h4>AA sequence of ORF-1:<br></h4>
+
-
<table border="0">
+
-
<p align="justify"><td align="left">
+
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
+
-
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
+
-
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
+
-
NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY
+
-
GYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRN
+
-
KHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGE
+
-
MIKAGTLTLEEVRRKFNNGEINFTG
+
-
 
+
-
 
+
-
 
+
-
 
+
-
<br><br></p></td></table>
+
-
 
+
-
<h4>Commented GenBank file</h4>
+
-
<li> <a href="https://static.igem.org/mediawiki/2009/4/46/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.txt">His+FluA+shortlinker+FokA_pMA.txt</a></li>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
 
+
-
</div><a name="9"></a>
+
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+shortlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
+
-
</div>
+
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
<table border="5">
+
<table border="5" style= "width: 100%;">
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/b/bc/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokI_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bshortlinker%2BFokI_pMA.jpg" name="His+FluA+shortlinker+Foki in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/d/d9/Freiburg09_FokA_pMA.jpg/600px-Freiburg09_FokA_pMA.jpg" name="FokA in pMA Vector" width="480" height="480" />
-
<br><p align="center">His+FluA+shortlinker+Foki in pMA Vector</p><br>
+
<br><p align="center">FokA in pMA Vector</p>
-
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243013">link to part registry</a>
+
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243000">link to part registry</a>
</td>
</td>
<td align="left">
<td align="left">
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
ATGGCCGGC
+
ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
+
ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG
-
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
+
AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGA
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
+
TGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTG
-
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
+
ATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGAT
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
+
GAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAA
-
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
+
CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAAT
-
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
+
TCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACC
-
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
+
CGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGA
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
+
GCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAG
-
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
+
AAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
-
GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG
+
-
CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA
+
-
GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG
+
-
AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC
+
-
CGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG
+
-
CGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTG
+
-
GTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAAC
+
-
AAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCAC
+
-
CGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAG
+
-
CCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTG
+
-
AGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCT
+
-
GACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTA
+
-
CCGGTTAA
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
</td>
</td>
Line 762: Line 239:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
+
MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL
-
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
+
GGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNE
-
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
+
WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTL
-
NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY
+
TLEEVRRKFNNGEINFTG
-
GYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRN
+
-
KHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGE
+
-
MIKAGTLTLEEVRRKFNNGEINFTG
+
-
 
+
-
 
+
-
 
+
-
 
+
<br><br></p></td></table>
<br><br></p></td></table>
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/7/7d/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokI_pMA.txt">His+FluA+shortlinker+Foki_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/3/3a/Freiburg09_FokA_pMA.txt">FokA_pMA.txt</a></li>
<br>
<br>
<br>
<br>
<br>
<br>
-
</div><a name="10"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
<table border="5">
+
<a name="3"></a>
-
<tr>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Foki_pMA<span class="art-PostHeader"></span> </h3>
-
<td align="center">
+
-
<img src="https://static.igem.org/mediawiki/2009/thumb/7/7d/Freiburg09_His%2BDig%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BDig%2Blonglinker%2BFokA_pMA.jpg" name="His+Dig+longlinker+FokA in pMA Vector" width="480" height="480" />
+
-
<br><p align="center">His+Dig+longlinker+FokA in pMA Vector</p>
+
-
</td>
+
-
<td align="left">
+
-
nucleotid sequence of ORF-1:<br>
+
-
 
+
-
ATGGCCGGC
+
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
+
-
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
+
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
+
-
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
+
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
+
-
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
+
-
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
+
-
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
+
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
+
-
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
+
-
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
+
-
TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC
+
-
CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
+
-
TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
+
-
ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
+
-
TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
+
-
ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA
+
-
ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG
+
-
TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG
+
-
TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT
+
-
AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT
+
-
TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA
+
-
CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT
+
-
TTTACCGGTTAA
+
-
 
+
-
</td>
+
-
</tr>
+
-
</table>
+
-
<br>
+
-
<h4>AA sequence of ORF-1:<br></h4>
+
-
<table border="0">
+
-
<p align="justify"><td align="left">
+
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
+
-
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
+
-
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
+
-
NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
+
-
TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP
+
-
IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK
+
-
TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
-
 
+
-
 
+
-
 
+
-
<br><br></p></td></table>
+
-
 
+
-
<h4>Commented GenBank file</h4>
+
-
<li> <a href="https://static.igem.org/mediawiki/2009/0/07/Freiburg09_His%2BDig%2Blonglinker%2BFokA_pMA.txt">His+Dig+longlinker+FokA_pMA.txt</a></li>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
 
+
-
</div><a name="11"></a>
+
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
+
-
</div>
+
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
<table border="5">
+
<table border="5" style= "width: 100%;">
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/6/64/Freiburg09_His%2BDig%2Blonglinker%2BFoki_pMA.JPG/600px-Freiburg09_His%2BDig%2Blonglinker%2BFoki_pMA.JPG" name="His+Dig+longlinker+Foki in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/6/68/Freiburg09_Foki_pMA.jpg/600px-Freiburg09_Foki_pMA.jpg" name="Foki in pMA Vector" width="480" height="480" />
-
<br><p align="center">His+Dig+longlinker+Foki in pMA Vector</p>
+
<br><p align="center">Foki in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243001">link to part registry</a>
</td>
</td>
<td align="left">
<td align="left">
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
ATGGCCGGC
+
ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
+
ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG
-
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
+
AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
+
AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG
-
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
+
CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
+
GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA
-
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
+
CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT
-
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
+
TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT
-
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
+
CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
+
ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG
-
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
+
AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
-
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
+
-
TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC
+
-
CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
+
-
TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
+
-
ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
+
-
TAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
+
-
ATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCG
+
-
ATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCG
+
-
TAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTG
+
-
TCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTAT
+
-
AAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGT
+
-
GCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCA
+
-
CCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAAT
+
-
TTTACCGGTTAA
+
Line 900: Line 290:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
+
MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL
-
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
+
GGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNE
-
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
+
WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTL
-
NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
+
TLEEVRRKFNNGEINFTG
-
TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLP
+
-
IGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHI
+
-
TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
-
 
+
-
 
+
-
 
+
<br><br></p></td></table>
<br><br></p></td></table>
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/e/ef/Freiburg09_His%2BDig%2Blonglinker%2BFoki_pMA.txt">His+Dig+longlinker+Foki_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/b/b8/Freiburg09_Foki_pMA.txt">Foki_pMA.txt</a></li>
<br>
<br>
<br>
<br>
Line 920: Line 304:
-
</div><a name="12"></a>
+
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
+
<a name="4"></a>
-
</div>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Dig_pMA<span class="art-PostHeader"></span> </h3>
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
<table border="5">
+
<table border="5" style= "width: 100%;">
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/f/fa/Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.jpg" name="Strep+FluA+Split+Foki in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/8/84/Freiburg09_Dig_pMA.jpg/600px-Freiburg09_Dig_pMA.jpg" name="Dig in pMA Vector" width="480" height="480" />
-
<br><p align="center">Strep+FluA+Split+Foki in pMA Vector</p>
+
<br><p align="center">Dig in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243003">link to part registry</a>
</td>
</td>
<td align="left">
<td align="left">
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
ATGGCC
+
ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT
-
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
+
GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGCAGGTGGCCG
-
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
+
CTTATCCCGACCACATCACCAAGTACGGCAAGTGCGGCTGGGCCGAGTAC
-
ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
+
ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTACAGCGTGATCCACGG
-
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
+
CAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTGTGGGCGACAGCAAGA
-
GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
+
TCGGCAAGATCTACCACAGCTACACCATCGGCGGCGTGACCCAGGTGGGC
-
CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
+
GTGAGCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACTT
-
GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
+
TTGCAGATACGACGAGGACAAGAAGGGCCACTGGGACGCCGTGTGGGTGC
-
CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
+
TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC
-
GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
+
TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA
-
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
+
CTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCAACTGGTCCCACCCCC
-
CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
+
AGTTCGAAAAGACCGGTTAA
-
ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA
+
-
GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT
+
-
GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA
+
-
TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG
+
-
TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG
+
-
TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG
+
-
TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT
+
-
CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA
+
-
ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG
+
-
AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC
+
-
CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG
+
-
TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA
+
-
CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC
+
-
GGTTAA
+
Line 972: Line 343:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
+
MAGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGKSVKVS
-
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
+
RYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIGYFCRY
-
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
+
DEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNSNW
-
CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ
+
SHPQFEKTG
-
DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG
+
-
QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN
+
-
SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
-
 
+
-
 
+
-
 
+
-
 
+
<br><br></p></td></table>
<br><br></p></td></table>
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/8/8d/Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.txt">Strep+FluA+Split+Foki_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/3/3d/Freiburg09_Dig_pMA.txt">Dig_pMA.txt</a></li>
<br>
<br>
<br>
<br>
<br>
<br>
-
 
+
<a name="5"></a>
-
</div><a name="13"></a>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;FluA_pMA<span class="art-PostHeader"></span> </h3>
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
+
-
</div>
+
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
<table border="5">
+
<table border="5" style= "width: 100%;">
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/c/c7/Freiburg09_His%2BDig%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BDig%2Bmiddellinker%2BFokA_pMA.jpg" name="His+Dig+middellinker+FokA in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/2/2a/Freiburg09_FluA_pMA.jpg/600px-Freiburg09_FluA_pMA.jpg" name="FluA in pMA Vector" width="480" height="480" />
-
<br><p align="center">His+Dig+middellinker+FokA in pMA Vector</p>
+
<br><p align="center">FluA in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K157004">link to part registry</a>
</td>
</td>
<td align="left">
<td align="left">
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
ATGGCCGGC
+
ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCA
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
+
AGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTAC
-
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
+
ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGG
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
+
CAAAGAATACTTCATGGAAGGCACCGCCTACCCCGTGGGCGACAGCAAGA
-
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
+
TCGGCAAGATCTACCACAGCCGGACCGTGGGCGGCTACACCAGAAAGACC
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
+
GTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACAG
-
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
+
CTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGC
-
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
+
TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC
-
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
+
TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
+
CTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCGGTTAA
-
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
+
-
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
+
-
TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA
+
-
CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC
+
-
GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC
+
-
ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC
+
-
TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG
+
-
TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT
+
-
GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT
+
-
CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA
+
-
AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG
+
-
ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA
+
-
AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG
+
-
AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
+
Line 1,045: Line 394:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
+
MAGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGKSVKVS
-
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
+
RYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIGYSCRY
-
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
+
DEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNTG
-
NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR
+
-
ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA
+
-
DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN
+
-
GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
-
 
+
-
 
+
-
 
+
-
 
+
Line 1,061: Line 402:
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/8/8a/Freiburg09_His%2BDig%2Bmiddellinker%2BFokA_pMA.txt">His+Dig+middellinker+FokA_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/7/71/Freiburg09_FluA_pMA.txt">FluA_pMA.txt</a></li>
<br>
<br>
<br>
<br>
Line 1,067: Line 408:
-
</div><a name="14"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/3/3b/Freiburg09_His%2BDig%2Bmiddellinker%2BFokI_pMA.jpg/600px-Freiburg09_His%2BDig%2Bmiddellinker%2BFokI_pMA.jpg" name="His+Dig+middellinker+Foki in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">His+Dig+middellinker+Foki in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
ATGGCCGGC
 
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 
-
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
 
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
 
-
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
 
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
 
-
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
 
-
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
 
-
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 
-
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
 
-
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
 
-
TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA
 
-
CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC
 
-
GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC
 
-
ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC
 
-
AGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG
 
-
TGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCT
 
-
GATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCT
 
-
GAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCA
 
-
AATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTG
 
-
ACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGA
 
-
GGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGG
 
-
AAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
 
-
</td>
+
<a name="6"></a>
-
</tr>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;DsbA_pMA<span class="art-PostHeader"></span> </h3>
-
</table>
+
-
<br>
+
-
<h4>AA sequence of ORF-1:<br></h4>
+
-
<table border="0">
+
-
<p align="justify"><td align="left">
+
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
+
-
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
+
-
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
+
-
NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR
+
-
ILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQA
+
-
DEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSN
+
-
GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
-
 
+
-
 
+
-
<br><br></p></td></table>
+
-
 
+
-
<h4>Commented GenBank file</h4>
+
-
<li> <a href="https://static.igem.org/mediawiki/2009/9/9f/Freiburg09_His%2BDig%2Bmiddellinker%2BFokI_pMA.txt">His+Dig+middellinker+Foki_pMA.txt</a></li>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
</div><a name="15"></a>
+
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
+
-
</div>
+
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
<table border="5">
+
<table border="5" style= "width: 100%;">
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/5/58/Freiburg09_His%2BDig%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BDig%2Bshortlinker%2BFokA_pMA.jpg" name="His+Dig+shortlinker+FokA in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/d/db/Freiburg09_DsbA_pMA.jpg/600px-Freiburg09_DsbA_pMA.jpg" name="DsbA in pMA Vector" width="480" height="480" />
-
<br><p align="center">His+Dig+shortlinker+FokA in pMA Vector</p>
+
<br><p align="center">DsbA in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243002">link to part registry</a>
</td>
</td>
<td align="left">
<td align="left">
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
ATGGCCGGC
+
ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
ATCGGCGACCGGTTA
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
+
-
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
+
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
+
-
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
+
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
+
-
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
+
-
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
+
-
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
+
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
+
-
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
+
-
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC
+
-
AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA
+
-
TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC
+
-
AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT
+
-
GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCAT
+
-
TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAG
+
-
CCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAG
+
-
CGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGA
+
-
ATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCG
+
-
TGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAAC
+
-
CATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGAT
+
-
TGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCC
+
-
GTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
+
-
 
+
-
 
+
-
 
+
</td>
</td>
</tr>
</tr>
Line 1,184: Line 438:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
+
MKKIWLALAGLVLAFSASATG
-
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
+
-
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
+
-
NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM
+
-
KVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQ
+
-
RYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVL
+
-
SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
-
 
+
-
 
+
-
 
+
<br><br></p></td></table>
<br><br></p></td></table>
-
 
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/d/d2/Freiburg09_His%2BDig%2Bshortlinker%2BFokA_pMA.txt">His+Dig+shortlinker+FokA_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/1/1b/Freiburg09_DsbA_pMA.txt">DsbA_pMA.txt</a></li>
<br>
<br>
<br>
<br>
<br>
<br>
-
</div><a name="16"></a>
+
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+shortlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
+
<a name="7"></a>
-
</div>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;StrepTag_pMA (iGEM team Freiburg 08)<span class="art-PostHeader"></span> </h3>
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
<table border="5">
+
<table border="5" style= "width: 100%;">
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/1/15/Freiburg09_His%2BDig%2Bshortlinker%2BFoki_pMA.jpg/600px-Freiburg09_His%2BDig%2Bshortlinker%2BFoki_pMA.jpg" name="His+Dig+shortlinker+Foki in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/7/74/Freiburg09_StrepTag_pMA.jpg/600px-Freiburg09_StrepTag_pMA.jpg" name="StrepTag in pMA Vector" width="480" height="480" />
-
<br><p align="center">His+Dig+shortlinker+Foki in pMA Vector</p>
+
<br><p align="center">StrepTag in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K157012">link to part registry</a>
</td>
</td>
<td align="left">
<td align="left">
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
 
+
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGTT
-
ATGGCCGGC
+
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
+
-
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
+
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
+
-
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
+
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
+
-
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
+
-
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
+
-
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
+
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
+
-
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
+
-
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC
+
-
AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA
+
-
TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC
+
-
AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT
+
-
GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCAT
+
-
TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAG
+
-
CGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAA
+
-
CGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGA
+
-
ATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCG
+
-
TGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAAC
+
-
CATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGAT
+
-
CGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCC
+
-
GTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
+
-
 
+
-
 
+
Line 1,254: Line 472:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
+
MAGWSHPQFEKTG
-
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
+
-
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
+
-
NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM
+
-
KVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEME
+
-
RYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVL
+
-
SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
-
 
+
-
 
+
-
 
+
<br><br></p></td></table>
<br><br></p></td></table>
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/b/b5/Freiburg09_His%2BDig%2Bshortlinker%2BFoki_pMA.txt">His+Dig+shortlinker+Foki_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/2/22/Freiburg09_StrepTag_pMA.txt">StrepTag_pMA.txt</a></li>
<br>
<br>
<br>
<br>
Line 1,274: Line 483:
-
</div><a name="17"></a>
+
<a name="8"></a>
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;HisTag_pMA (iGEM team Freiburg 08)<span class="art-PostHeader"></span> </h3>
-
</div>
+
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
<table border="5">
+
<table border="5" style= "width: 100%;">
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/d/d7/Freiburg09_His%2BDig%2BSplit%2BFokI_pMA.jpg/600px-Freiburg09_His%2BDig%2BSplit%2BFokI_pMA.jpg" name="His+Dig+Split+Foki in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/8/8a/Freiburg09_HisTag_pMA.jpg/600px-Freiburg09_HisTag_pMA.jpg" name="HisTag in pMA Vector" width="480" height="480" />
-
<br><p align="center">His+Dig+Split+Foki in pMA Vector</p><br>
+
<br><p align="center">HisTag in pMA Vector</p>
-
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243018">link to part registry</a>
+
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K157011">link to part registry</a>
</td>
</td>
<td align="left">
<td align="left">
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
 
+
GCCGGCCATCATCATCATCATCATACCGGT
-
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG
+
-
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
+
-
ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
+
-
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
+
-
GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
+
-
CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
+
-
ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
+
-
CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
+
-
GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
+
-
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
+
-
CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
+
-
ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC
+
-
TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA
+
-
ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG
+
-
TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA
+
-
GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG
+
-
CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTT
+
-
ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCG
+
-
TATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACG
+
-
TTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAAT
+
-
GGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTG
+
-
AGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCA
+
-
TATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCG
+
-
GTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGT
+
-
AAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
+
-
 
+
-
 
+
Line 1,328: Line 509:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
+
AGHHHHHHTG
-
EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
+
-
IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
+
-
CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE
+
-
LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKA
+
-
YSGGYNLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA
+
-
QLTRLNHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
-
 
+
-
 
+
-
 
+
Line 1,343: Line 515:
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/1/11/Freiburg09_His%2BDig%2BSplit%2BFokI_pMA.txt">His+Dig+Split+Foki_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/3/34/Freiburg09_HisTag_pMA.txt">HisTag_pMA.txt</a></li>
<br>
<br>
<br>
<br>
Line 1,349: Line 521:
-
</div><a name="18"></a>
+
<a name="9"></a>
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;shortlinker26_pMA <span class="art-PostHeader"></span> </h3>
-
</div>
+
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
<table border="5">
+
<table border="5" style= "width: 100%;">
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/9/9f/Freiburg09_His%2BFluA%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BFluA%2Blonglinker%2BFokA_pMA.jpg" name="His+FluA+longlinker+FokA in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/7/7d/Freiburg09_Shortlinker26_pMA.jpg/600px-Freiburg09_Shortlinker26_pMA.jpg" name="shortlinker26 in pMA Vector" width="480" height="480" />
-
<br><p align="center">His+FluA+longlinker+FokA in pMA Vector</p>
+
<br><p align="center">shortlinker26 in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243004">link to part registry</a>
</td>
</td>
<td align="left">
<td align="left">
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
 
+
ATGGCCGGCGGTGGTTCTGGTACCGGT
-
ATGGCCGGC
+
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
+
-
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
+
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
+
-
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
+
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
+
-
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
+
-
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
+
-
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
+
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
+
-
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
+
-
GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT
+
-
GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC
+
-
TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC
+
-
GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT
+
-
CGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATAC
+
-
CGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATA
+
-
GCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTAT
+
-
GTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTG
+
-
GAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCG
+
-
GCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAA
+
-
ACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGG
+
-
TGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAAT
+
-
TCAACAATGGGGAGATCAATTTTACCGGTTAA
+
-
 
+
-
 
+
-
 
+
Line 1,402: Line 546:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
+
MAGGGSGTG
-
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
+
-
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
+
-
NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
+
-
MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRY
+
-
VKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSV
+
-
EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
-
 
+
-
 
+
-
 
+
-
 
+
Line 1,418: Line 552:
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/b/be/Freiburg09_His%2BFluA%2Blonglinker%2BFokA_pMA.txt">His+FluA+longlinker+FokA_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/0/0b/Freiburg09_Shortlinker26_pMA.txt">shortlinker26_pMA.txt</a></li>
<br>
<br>
<br>
<br>
Line 1,424: Line 558:
-
</div><a name="19"></a>
+
<a name="10"></a>
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Middellinker38_pMA <span class="art-PostHeader"></span> </h3>
-
</div>
+
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
 
+
<table border="5" style= "width: 100%;">
-
<table border="5">
+
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/6/6c/Freiburg09_His%2BFluA%2Blonglinker%2BFokI_pMA.jpg/600px-Freiburg09_His%2BFluA%2Blonglinker%2BFokI_pMA.jpg" name="His+FluA+longlinker+Foki in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/f/f6/Freiburg09_Middellinker38_pMA.jpg/600px-Freiburg09_Middellinker38_pMA.jpg" name="Middellinker38 in pMA Vector" width="480" height="480" />
-
<br><p align="center">His+FluA+longlinker+Foki in pMA Vector</p><br>
+
<br><p align="center">Middellinker38 in pMA Vector</p>
-
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243016">link to part registry</a>
+
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243005">link to part registry</a>
</td>
</td>
<td align="left">
<td align="left">
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
 
+
ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGT
-
ATGGCCGGC
+
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
+
-
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
+
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
+
-
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
+
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
+
-
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
+
-
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
+
-
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
+
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
+
-
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
+
-
GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT
+
-
GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC
+
-
TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC
+
-
GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT
+
-
CGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATAC
+
-
CGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATT
+
-
CTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTAT
+
-
GTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTG
+
-
GAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCG
+
-
GCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATC
+
-
ACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGG
+
-
AGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAAT
+
-
TCAACAATGGCGAGATCAATTTTACCGGTTAA
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
</td>
</td>
Line 1,479: Line 581:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
+
MAGGGSGGGSGTG
-
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
+
-
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
+
-
NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
+
-
MEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERY
+
-
VEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSV
+
-
EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
-
 
+
-
 
+
-
 
+
<br><br></p></td></table>
<br><br></p></td></table>
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/7/77/Freiburg09_His%2BFluA%2Blonglinker%2BFokI_pMA.txt">His+FluA+longlinker+Foki_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/d/df/Freiburg09_Middellinker38_pMA.txt">Middellinker38_pMA.txt</a></li>
<br>
<br>
<br>
<br>
Line 1,501: Line 594:
-
 
+
<a name="11"></a>
-
</div><a name="20"></a>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;LongLinkerA50_pMA <span class="art-PostHeader"></span> </h3>
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
+
-
</div>
+
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
 
+
<table border="5" style= "width: 100%;">
-
<table border="5">
+
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/e/ef/Freiburg09_His%2BFluA%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_His%2BFluA%2BSplit%2BFokA_pMA.jpg" name="His+FluA+Split+FokA in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/e/ec/Freiburg09_LongLinkerA50_pMA.jpg/600px-Freiburg09_LongLinkerA50_pMA.jpg" name="LongLinkerA50 in pMA Vector" width="480" height="480" />
-
<br><p align="center">His+FluA+Split+FokA in pMA Vector</p>
+
<br><p align="center">LongLinkerA50 in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243006">link to part registry</a>
</td>
</td>
<td align="left">
<td align="left">
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
 
+
ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGT
-
ATGGCC
+
-
GGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTG
+
-
CCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCA
+
-
AGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAG
+
-
TGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAG
+
-
ATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACC
+
-
CCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC
+
-
GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAA
+
-
CTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACT
+
-
GGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCC
+
-
AAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCA
+
-
GAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACA
+
-
CCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATG
+
-
AATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA
+
-
CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC
+
-
GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC
+
-
ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC
+
-
TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG
+
-
TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT
+
-
GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT
+
-
CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA
+
-
AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG
+
-
ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA
+
-
AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG
+
-
AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
+
-
 
+
</td>
</td>
Line 1,552: Line 617:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEG
+
MAGGGSGGGSGTG
-
KSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYII
+
-
GYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACK
+
-
VNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR
+
-
ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA
+
-
DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN
+
-
GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
-
 
+
<br><br></p></td></table>
<br><br></p></td></table>
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/5/53/Freiburg09_His%2BFluA%2BSplit%2BFokA_pMA.txt">His+FluA+Split+FokA_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/d/d2/Freiburg09_LongLinkerA50_pMA.txt">LongLinkerA50_pMA.txt</a></li>
<br>
<br>
<br>
<br>
Line 1,571: Line 629:
-
</div><a name="21"></a>
+
<a name="12"></a>
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;GSAT_linker_pMA <span class="art-PostHeader"></span> </h3>
-
</div>
+
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
 
+
<table border="5" style= "width: 100%;">
-
<table border="5">
+
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/8/8b/Freiburg09_Strep%2BDig%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Blonglinker%2BFokA_pMA.jpg" name="Strep+Dig+longlinker+FokA in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/1/11/Freiburg09_GSAT_linker_pMA.jpg/600px-Freiburg09_GSAT_linker_pMA.jpg" name="GSAT_linker in pMA Vector" width="480" height="480" />
-
<br><p align="center">Strep+Dig+longlinker+FokA in pMA Vector</p>
+
<br><p align="center">GSAT_linker in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243029">link to part registry</a>
</td>
</td>
<td align="left">
<td align="left">
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
 
+
GAGCTCGAATTCGCGGCCGCTTCTAGATGGCCGGCGGTGGTTCTGCCGGT
-
ATGGCC
+
GGCTCCGGTTCTGGCTCCAGCGGTGGCAGCTCTGGTGCGTCCGGCACGGG
-
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
+
TACTGCGGGTGGCACTGGCAGCGGTTCCGGTACTGGCTCTGGCACCGGTA
-
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
+
ATACTAGTAGCGGCCGCTGCAGGGTACC
-
ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
+
-
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
+
-
GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
+
-
CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
+
-
ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
+
-
CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
+
-
GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
+
-
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
+
-
CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
+
-
ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT
+
-
GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA
+
-
GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA
+
-
TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA
+
-
ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA
+
-
TCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCC
+
-
CGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTAT
+
-
AACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAA
+
-
CCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATC
+
-
CGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAA
+
-
GGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAA
+
-
TGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCA
+
-
AAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGG
+
-
GAGATCAATTTTACCGGTTAA
+
-
 
+
Line 1,623: Line 656:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
+
ELEFAAASRWPAVVLPVAPVLAPAVAALVRPARVLRVALAAVPVLALAPVILVAAAAGY
-
EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
+
-
IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
+
-
CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA
+
-
RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGY
+
-
NLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL
+
-
NHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
-
 
+
Line 1,636: Line 662:
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/2/22/Freiburg09_Strep%2BDig%2Blonglinker%2BFokA_pMA.txt">Strep+Dig+longlinker+FokA_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/1/14/Freiburg09_GSAT_linker_pMA.txt">GSAT_linker_pMA.txt</a></li>
<br>
<br>
<br>
<br>
<br>
<br>
-
</div><a name="22"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
+
<a name="13"></a>
-
<table border="5">
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;SEG_Linker_pMA <span class="art-PostHeader"></span> </h3>
-
<tr>
+
-
<td align="center">
+
-
<img src="https://static.igem.org/mediawiki/2009/thumb/6/65/Freiburg09_Strep%2BDig%2Blonglinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Blonglinker%2BFokI_pMA.jpg" name="Strep+Dig+longlinker+Foki in pMA Vector" width="480" height="480" />
+
-
<br><p align="center">Strep+Dig+longlinker+Foki in pMA Vector <br>
+
-
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243011">link to part registry</a></li>
+
-
</p>
+
-
</td>
+
-
<td align="left">
+
-
nucleotid sequence of ORF-1:<br>
+
-
 
+
-
ATGGCC
+
-
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
+
-
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
+
-
ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
+
-
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
+
-
GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
+
-
CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
+
-
ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
+
-
CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
+
-
GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
+
-
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
+
-
CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
+
-
ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT
+
-
GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA
+
-
GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA
+
-
TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA
+
-
ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA
+
-
TCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCC
+
-
CGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTAT
+
-
AATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAA
+
-
TCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATC
+
-
CGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAA
+
-
GGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAA
+
-
TGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCA
+
-
AAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGC
+
-
GAGATCAATTTTACCGGTTAA
+
-
 
+
-
 
+
-
 
+
-
 
+
-
</td>
+
-
</tr>
+
-
</table>
+
-
<br>
+
-
<h4>AA sequence of ORF-1:<br></h4>
+
-
<table border="0">
+
-
<p align="justify"><td align="left">
+
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
+
-
EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
+
-
IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
+
-
CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA
+
-
RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGY
+
-
NLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL
+
-
NHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
-
 
+
-
 
+
-
 
+
-
 
+
-
<br><br></p></td></table>
+
-
 
+
-
<h4>Commented GenBank file</h4>
+
-
<li> <a href="https://static.igem.org/mediawiki/2009/4/4c/Freiburg09_Strep%2BDig%2Blonglinker%2BFokI_pMA.txt">Strep+Dig+longlinker+Foki_pMA.txt</a></li>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
 
+
-
 
+
-
 
+
-
</div><a name="23"></a>
+
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
+
-
</div>
+
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
 
+
<table border="5" style= "width: 100%;">
-
<table border="5">
+
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/9/94/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.jpg" name="Strep+Dig+middellinker+FokA in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/0/09/Freiburg09_SEG_Linker.jpg/600px-Freiburg09_SEG_Linker.jpg" name="SEG_Linker in pMA Vector" width="480" height="480" />
-
<br><p align="center">Strep+Dig+middellinker+FokA in pMA Vector</p>
+
<br><p align="center">SEG_Linker in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243030">link to part registry</a>
</td>
</td>
<td align="left">
<td align="left">
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
 
+
GAGCTCGAATTCGCGGCCGCTTCTAGATGGCCGGCGGTGGTTCTGGCGGC
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
+
GGTTCTGAAGGTGGCGGCTCCGAAGGCGGCGGCAGCGAGGGCGGTGGTAG
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
+
CGAAGGTGGTGGCTCCGAGGGTGGCGGTTCCGGCGGCGGTAGCACCGGTA
-
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
+
ATACTAGTAGCGGCCGCTGCAGGGTACC
-
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
+
-
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
+
-
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
+
-
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
+
-
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
+
-
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
+
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
+
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
+
-
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
+
-
GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA
+
-
ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC
+
-
TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA
+
-
GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG
+
-
TGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCG
+
-
ATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTG
+
-
CCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGAC
+
-
TCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA
+
-
GCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAAC
+
-
TATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGC
+
-
CGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCG
+
-
GGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATC
+
-
AATTTTACCGGTTAA
+
-
 
+
-
 
+
-
 
+
Line 1,770: Line 695:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
+
ELEFAAASRWPAVVLAAVLKVAAPKAAAARAVVAKVVAPRVAVPAAVAPVILVAAAAGY
-
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
+
-
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
+
-
AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN
+
-
STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNL
+
-
PIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH
+
-
KTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
-
 
+
-
 
+
-
 
+
-
 
+
<br><br></p></td></table>
<br><br></p></td></table>
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/9/9f/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.txt">Strep+Dig+middellinker+FokA_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/d/d7/Freiburg09_SEG_Linker.txt">SEG_Linker_pMA.txt</a></li>
<br>
<br>
<br>
<br>
Line 1,791: Line 706:
-
 
+
<a name="14"></a>
-
</div><a name="24"></a>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Split_Linker_pMA (iGEM team Freiburg 08)<span class="art-PostHeader"></span> </h3>
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
+
-
</div>
+
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
 
+
<table border="5" style= "width: 100%;">
-
<table border="5">
+
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/7/79/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.jpg" name="Strep+Dig+middellinker+Foki in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/1/13/Freiburg09_Split-Linker_pMA.jpg/600px-Freiburg09_Split-Linker_pMA.jpg" name="Split_Linker in pMA Vector" width="480" height="480" />
-
<br><p align="center">Strep+Dig+middellinker+Foki in pMA Vector<br>
+
<br><p align="center">Split_Linker in pMA Vector</p>
-
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243012">link to part registry</a>
+
<li> <a href="http://partsregistry.org/Part:BBa_K157009">link to part registry</a>
-
</p>
+
</td>
</td>
<td align="left">
<td align="left">
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
 
+
ATGGCCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGT
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
+
TATGAATCACACCGGT
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
+
-
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
+
-
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
+
-
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
+
-
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
+
-
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
+
-
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
+
-
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
+
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
+
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
+
-
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
+
-
GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA
+
-
ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC
+
-
TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA
+
-
GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG
+
-
TGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCG
+
-
ATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTG
+
-
CCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGAC
+
-
CCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA
+
-
GTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAAC
+
-
TATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGC
+
-
AGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAG
+
-
GCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATC
+
-
AATTTTACCGGTTAA
+
-
 
+
-
 
+
-
 
+
-
 
+
</td>
</td>
Line 1,847: Line 730:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
+
MAGRPACKIPNDLKQKVMNHTG
-
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
+
-
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
+
-
AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN
+
-
STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNL
+
-
PIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH
+
-
ITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
<br><br></p></td></table>
<br><br></p></td></table>
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/7/73/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.txt">Strep+Dig+middellinker+Foki_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/2/21/Freiburg09_Split-Linker_pMA.txt">Split_Linker_pMA.txt</a></li>
<br>
<br>
<br>
<br>
Line 1,864: Line 741:
-
 
+
<a name="15"></a>
-
</div><a name="25"></a>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;YFP(Venus)_pGA14 (iGEM team Freiburg 08) <span class="art-PostHeader"></span> </h3>
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
+
-
</div>
+
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
 
+
<table border="5" style= "width: 100%;">
-
<table border="5">
+
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/9/97/Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg" name="Strep+FluA+Split+FokA in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/6/61/Freiburg09_PGA14_YFP%28Venus%29.jpg/600px-Freiburg09_PGA14_YFP%28Venus%29.jpg" name="YFP(Venus) in pGA14 Vector" width="480" height="480" />
-
<br><p align="center">Strep+FluA+Split+FokA in pMA Vector</p>
+
<br><p align="center">YFP(Venus) in pGA14 Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K157007">link to part registry</a>
</td>
</td>
<td align="left">
<td align="left">
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
 
+
ATGGCCGGCATGAGCAAAGGCGAAGAACTGTTTACCGGCGTGGTGCCGAT
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
+
TCTGGTGGAACTGGATGGTGATGTGAACGGCCATAAATTTAGCGTGAGCG
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
+
GCGAAGGCGAAGGTGATGCGACCTATGGCAAACTGACCCTGAAACTGATT
-
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
+
TGCACCACCGGCAAACTGCCGGTTCCGTGGCCGACCCTGGTTACCACCCT
-
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
+
GGGCTATGGCCTGCAATGCTTTGCGCGTTATCCGGATCATATGAAACAGC
-
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
+
ACGATTTCTTTAAAAGCGCCATGCCGGAAGGCTATGTGCAGGAACGCACC
-
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
+
ATCTTTTTTAAAGATGATGGCAACTATAAAACCCGTGCGGAAGTGAAATT
-
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
+
TGAAGGCGATACCCTGGTGAACCGTATTGAACTGAAAGGCATCGATTTCA
-
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
+
AAGAAGATGGCAACATTCTGGGCCATAAACTGGAATATAACTACAACAGC
-
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
+
CATAACGTGTATATCACCGCGGATAAACAGAAAAACGGCATCAAAGCGAA
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
+
CTTTAAAATCCGCCACAACATTGAAGATGGCGGCGTGCAGCTGGCCGATC
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
+
ATTATCAGCAGAACACCCCGATTGGTGATGGCCCGGTGCTGCTGCCGGAT
-
AAGTGAACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAG
+
AACCATTATCTGAGCTACCAGAGCAAACTGAGCAAAGATCCGAACGAAAA
-
CAGAAAGTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATC
+
ACGTGACCATATGGTGCTGCTGGAATTTGTGACCGCGGCCGGTATTACCC
-
CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
+
ATGGCATGGATGAACTGTATAAAACCGGTTAA
-
TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
+
-
ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
+
-
TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
+
-
ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA
+
-
ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG
+
-
TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG
+
-
TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT
+
-
AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT
+
-
TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA
+
-
CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT
+
-
TTTACCGGTTAA
+
-
 
+
-
 
+
</td>
</td>
Line 1,916: Line 778:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
+
MAGMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPW
-
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
+
PTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGD
-
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
+
TLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQ
-
AACKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
+
LADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITHGMDELY
-
TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP
+
KTG
-
IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK
+
-
TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
Line 1,928: Line 788:
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/7/73/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.txt">Strep+Dig+middellinker+Foki_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/2/2e/Freiburg09_PGA14_YFP%28Venus%29.txt">YFP(Venus)_pGA14.txt</a></li>
<br>
<br>
<br>
<br>
Line 1,934: Line 794:
-
 
+
<a name="19"></a>
-
</div><a name="26"></a>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Fos_pMA (iGEM team Freiburg 08) <span class="art-PostHeader"></span> </h3>
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
+
-
</div>
+
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
 
+
<table border="5" style= "width: 100%;">
-
<table border="5">
+
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/9/97/Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg" name="Strep+FluA+Split+FokA in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/c/ca/Freiburg09_Fos_pMA.jpg/600px-Freiburg09_Fos_pMA.jpg" name="Fos in pMA Vector" width="480" height="480" />
-
<br><p align="center">Strep+FluA+Split+FokA in pMA Vector</p>
+
<br><p align="center">Fos) in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243027">link to part registry</a>
</td>
</td>
<td align="left">
<td align="left">
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
 
+
ATGGCCGGCCACCATCACCACCACCATGGATCCGGTGAAGAAAAACGCCG
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
+
TATCCGTCGTGAGCGTAACAAAATGGCAGCAGCCAAATCCCGTAATCGCC
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
+
GTCGTGAGCTGACAGACACACTGCAAGCCGAAACCGATCAACTGGAGGAC
-
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
+
GAGAAAAGTGCTCTGCAAACTGAGATTGCCAATCTGCTGAAAGAGAAAGA
-
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
+
AAAACTGGAGTTCATCCTGGCAGCAACCGGTTAA
-
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
+
-
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
+
-
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
+
-
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
+
-
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
+
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
+
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
+
-
AAGTGAACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAG
+
-
CAGAAAGTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATC
+
-
CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
+
-
TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
+
-
ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
+
-
TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
+
-
ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA
+
-
ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG
+
-
TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG
+
-
TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT
+
-
AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT
+
-
TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA
+
-
CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT
+
-
TTTACCGGTTAA
+
Line 1,985: Line 822:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
+
MAGHHHHHHGSGEEKRRIRRERNKMAAAKSRNRRRELTDTLQAETDQLEDEKSALQTEIA
-
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
+
NLLKEKEKLEFILAATG
-
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
+
-
AACKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
+
-
TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP
+
-
IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK
+
-
TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
+
Line 1,998: Line 830:
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/6/6a/Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.txt">Strep+FluA+Split+FokA_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/8/80/Freiburg09_Fos_pMA.txt">Fos_pMA.txt</a></li>
<br>
<br>
<br>
<br>
Line 2,004: Line 836:
-
</div><a name="27"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/f/fb/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bshortlinker%2BFokA_pMA.jpg" name="Strep+Dig+shortlinker+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+Dig+shortlinker+FokA in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 
-
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
 
-
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 
-
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
 
-
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 
-
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
 
-
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
 
-
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
 
-
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
 
-
GGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCG
 
-
CCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCG
 
-
CCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTC
 
-
TTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAA
 
-
ACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTA
 
-
TCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAG
 
-
GCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACA
 
-
CATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGT
 
-
TCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAG
 
-
CTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGT
 
-
AGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACAC
 
-
TGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGT
 
-
TAA
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 
-
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 
-
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQD
 
-
RILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQ
 
-
ADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNS
 
-
NGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
+
<a name="16"></a>
-
<br><br></p></td></table>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;pJS418_Phagemid-dummy <span class="art-PostHeader"></span> </h3>
-
 
+
-
<h4>Commented GenBank file</h4>
+
-
<li> <a href="https://static.igem.org/mediawiki/2009/4/45/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokA_pMA.txt">Strep+Dig+shortlinker+FokA_pMA.txt</a></li>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
 
+
-
 
+
-
</div><a name="28"></a>
+
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+shortlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
+
-
</div>
+
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
 
+
<table border="5" style= "width: 100%;">
-
<table border="5">
+
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/4/4a/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bshortlinker%2BFokI_pMA.jpg" name="Strep+Dig+shortlinker+Foki in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/8/84/Freiburg09_PJS418_dummy.jpg/600px-Freiburg09_PJS418_dummy.jpg" name="pJS418 Vector with strong SD sequence" width="480" height="480" />
-
<br><p align="center">Strep+Dig+shortlinker+Foki in pMA Vector</p>
+
<br><p align="center">pJS418 Vector with strong SD sequence</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243034">link to part registry</a>
</td>
</td>
-
<td align="left">
+
<td align="center">
-
nucleotid sequence of ORF-1:<br>
+
-
 
+
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
+
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
+
-
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
+
-
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
+
-
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
+
-
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
+
-
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
+
-
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
+
-
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
+
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
+
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
+
-
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
+
-
GGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCG
+
-
CCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCG
+
-
CCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTC
+
-
TTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAA
+
-
ACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTA
+
-
TCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAG
+
-
GCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACA
+
-
TCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGT
+
-
TCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAG
+
-
CTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGT
+
-
TGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTC
+
-
TGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGT
+
-
TAA
+
-
 
+
-
 
+
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/3/3a/Freiburg09_PJS418_dummy.txt">pJS418.txt</a></li>
</td>
</td>
</tr>
</tr>
</table>
</table>
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 
-
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 
-
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQD
 
-
RILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQ
 
-
ADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNS
 
-
NGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/0/0f/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokI_pMA.txt">Strep+Dig+shortlinker+Foki_pMA.txt</a></li>
 
<br>
<br>
<br>
<br>
Line 2,141: Line 866:
-
 
+
<a name="17"></a>
-
</div><a name="29"></a>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;pJS419_Phagemid-dummy <span class="art-PostHeader"></span> </h3>
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
+
-
</div>
+
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
 
+
<table border="5" style= "width: 100%;">
-
<table border="5">
+
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/f/ff/Freiburg09_Strep%2BDig%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2BSplit%2BFokA_pMA.jpg" name="Strep+Dig+Split+FokA in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/b/b1/Freiburg09_PJS419_dummy.jpg/600px-Freiburg09_PJS419_dummy.jpg" name="pJS419 Vector with weak SD sequence" width="480" height="480" />
-
<br><p align="center">Strep+Dig+Split+FokA in pMA Vector</p><br>
+
<br><p align="center">pJS419 Vector with weak SD sequence</p>
-
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243017">link to part registry</a>
+
<li> <a href="http://partsregistry.org/Part:BBa_K243035">link to part registry</a>
</td>
</td>
-
<td align="left">
+
<td align="center">
-
nucleotid sequence of ORF-1:<br>
+
-
 
+
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
+
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
+
-
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
+
-
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
+
-
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
+
-
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
+
-
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
+
-
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
+
-
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
+
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
+
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
+
-
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGA
+
-
CCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACAC
+
-
CGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGA
+
-
AATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGT
+
-
ACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGT
+
-
CTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTG
+
-
CCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACC
+
-
AAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGAT
+
-
GCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGA
+
-
ACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTG
+
-
TTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCT
+
-
GAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGC
+
-
TGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTT
+
-
CGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
+
-
 
+
-
 
+
-
 
+
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/d/d9/Freiburg09_PJS419_dummy.txt">pJS419.txt</a></li>
</td>
</td>
</tr>
</tr>
</table>
</table>
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 
-
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 
-
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEY
 
-
IELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDT
 
-
KAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNY
 
-
KAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/9/95/Freiburg09_Strep%2BDig%2BSplit%2BFokA_pMA.txt">Strep+Dig+Split+FokA_pMA.txt</a></li>
 
<br>
<br>
<br>
<br>
Line 2,215: Line 892:
-
</div><a name="30"></a>
+
<a name="18"></a>
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;pBAD_dummy <span class="art-PostHeader"></span> </h3>
-
</div>
+
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
 
+
<table border="5" style= "width: 100%;">
-
<table border="5">
+
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/f/fe/Freiburg09_Strep%2BDig%2BSplit%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2BSplit%2BFokI_pMA.jpg" name="Strep+Dig+Split+Foki in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/6/69/Freiburg09_PBAD_dummy.jpg/600px-Freiburg09_PBAD_dummy.jpg" name="pBAD Vector" width="480" height="480" />
-
<br><p align="center">Strep+Dig+Split+Foki in pMA Vector</p><br>
+
<br><p align="center">pBAD Vector</p>
-
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243015">link to part registry</a>
+
<li> <a href="http://partsregistry.org/Part:BBa_K243037">link to part registry</a>
</td>
</td>
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 
-
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
 
-
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 
-
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
 
-
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 
-
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
 
-
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
 
-
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
 
-
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGA
 
-
CCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACAC
 
-
CGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGA
 
-
AATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGT
 
-
ACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGT
 
-
CTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTG
 
-
CCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACA
 
-
AAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGAT
 
-
GGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGA
 
-
ACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTG
 
-
TTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCT
 
-
GAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGC
 
-
TGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTT
 
-
CGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 
-
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 
-
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEY
 
-
IELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVAT
 
-
KAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNY
 
-
KAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/6/63/Freiburg09_Strep%2BDig%2BSplit%2BFokI_pMA.txt">Strep+Dig+Split+Foki_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
</div><a name="31"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/2/22/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Blonglinker%2BFokA_pMA.jpg" name="Strep+FluA+longlinker+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+FluA+longlinker+FokA in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
 
-
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 
-
ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
 
-
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 
-
GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
 
-
CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
 
-
GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
 
-
CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
 
-
GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 
-
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 
-
CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
 
-
ACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACC
 
-
GGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAA
 
-
ATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTA
 
-
CCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTC
 
-
TATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGC
 
-
CATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCA
 
-
AAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATG
 
-
CAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAA
 
-
CGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGT
 
-
TCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTG
 
-
AACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCT
 
-
GATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTC
 
-
GCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
GWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
 
-
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
 
-
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
 
-
CKVNNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILE
 
-
MKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEM
 
-
QRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAV
 
-
LSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/d/d4/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokA_pMA.txt">Strep+FluA+longlinker+FokA_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/1/1c/Freiburg09_PBAD_dummy.txt">pBAD_dummy.txt</a></li>
-
<br>
+
-
<br>
+
-
<br>
+
-
 
+
-
 
+
-
</div><a name="32"></a>
+
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
+
-
</div>
+
-
<div class="art-PostContent">
+
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
+
-
 
+
-
 
+
-
<table border="5">
+
-
<tr>
+
-
<td align="center">
+
-
<img src="https://static.igem.org/mediawiki/2009/thumb/4/47/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.jpg" name="Strep+FluA+longlinker+Foki in pMA Vector" width="480" height="480" />
+
-
<br><p align="center">Strep+FluA+longlinker+Foki in pMA Vector</p>
+
-
</td>
+
-
<td align="left">
+
-
nucleotid sequence of ORF-1:<br>
+
-
 
+
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
+
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
+
-
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
+
-
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
+
-
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
+
-
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
+
-
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
+
-
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
+
-
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
+
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
+
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
+
-
AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCT
+
-
GGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA
+
-
ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA
+
-
ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG
+
-
AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC
+
-
AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG
+
-
CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT
+
-
GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA
+
-
CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT
+
-
TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT
+
-
CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA
+
-
ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG
+
-
AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
+
-
 
+
-
 
+
</td>
</td>
</tr>
</tr>
</table>
</table>
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 
-
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 
-
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRI
 
-
LEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQAD
 
-
EMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNG
 
-
AVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/c/ce/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.txt">Strep+FluA+longlinker+Foki_pMA.txt</a></li>
 
<br>
<br>
<br>
<br>
Line 2,421: Line 917:
-
</div><a name="33"></a>
+
<a name="20"></a>
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
+
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;pEX_CatInsert <span class="art-PostHeader"></span> </h3>
-
</div>
+
<div class="art-PostContent">
<div class="art-PostContent">
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
 
+
<table border="5" style= "width: 100%;">
-
<table border="5">
+
<tr>
<tr>
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/d/d8/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.jpg" name="Strep+FluA+middellinker+FokA in pMA Vector" width="480" height="480" />
+
<img src="https://static.igem.org/mediawiki/2009/thumb/a/a6/Freiburg09_PEX_CATInsert.jpg/600px-Freiburg09_PEX_CATInsert.jpg" name="pEX Vector" width="480" height="480" />
-
<br><p align="center">Strep+FluA+middellinker+FokA in pMA Vector</p>
+
<br><p align="center">pEX Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243033">link to part registry</a>
</td>
</td>
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 
-
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
 
-
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 
-
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
 
-
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 
-
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
 
-
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
 
-
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
 
-
AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA
 
-
TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT
 
-
GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG
 
-
ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC
 
-
TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTA
 
-
TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCT
 
-
ATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGT
 
-
TATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATG
 
-
GTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGA
 
-
GCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCAT
 
-
AAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGG
 
-
CGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTA
 
-
AATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 
-
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 
-
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK
 
-
VMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
 
-
YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLS
 
-
VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/4/48/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.txt">Strep+FluA+middellinker+FokA_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
</div><a name="34"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
<td align="center">
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2009/thumb/b/b9/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.jpg" name="Strep+FluA+middellinker+Foki in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+FluA+middellinker+Foki in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 
-
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
 
-
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 
-
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
 
-
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 
-
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
 
-
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
 
-
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
 
-
AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA
 
-
TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT
 
-
GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG
 
-
ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC
 
-
TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTA
 
-
TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCGT
 
-
ATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACGT
 
-
TATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAATG
 
-
GTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGA
 
-
GCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCAT
 
-
ATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGG
 
-
TGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTA
 
-
AATTCAACAATGGCGAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 
-
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 
-
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK
 
-
VMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMER
 
-
YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLS
 
-
VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/a/a3/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.txt">Strep+FluA+middellinker+Foki_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/7/7a/Freiburg09_PEX_CATInsert.txt">pEX_CatInsert.txt</a></li>
-
<br>
+
-
<br>
+
-
<br>
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
</div><a name="35"></a>
+
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
+
-
</div>
+
-
<div class="art-PostContent">
+
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<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
+
-
 
+
-
 
+
-
<table border="5">
+
-
<tr>
+
-
<td align="center">
+
-
<img src="https://static.igem.org/mediawiki/2009/thumb/d/db/Freiburg09_Strep%2BFluA%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bshortlinker%2BFokA_pMA.jpg" name="Strep+FluA+shortlinker+FokA in pMA Vector" width="480" height="480" />
+
-
<br><p align="center">Strep+FluA+shortlinker+FokA in pMA Vector</p>
+
-
</td>
+
-
<td align="left">
+
-
nucleotid sequence of ORF-1:<br>
+
-
 
+
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
+
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
+
-
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
+
-
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
+
-
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
+
-
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
+
-
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
+
-
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
+
-
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
+
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
+
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
+
-
AAGTGAACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAG
+
-
GAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTA
+
-
TATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGG
+
-
AAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAA
+
-
CATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTC
+
-
CCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTT
+
-
ATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAG
+
-
AACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTA
+
-
TCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTA
+
-
AAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGC
+
-
AATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGAT
+
-
CAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATG
+
-
GGGAGATCAATTTTACCGGTTAA
+
-
 
+
</td>
</td>
</tr>
</tr>
</table>
</table>
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 
-
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 
-
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEF
 
-
FMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKE
 
-
NQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEEL
 
-
LIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/4/49/Freiburg09_Strep%2BFluA%2Bshortlinker%2BFokA_pMA.txt">Strep+FluA+shortlinker+FokA_pMA.txt</a></li>
 
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<br>
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Latest revision as of 03:38, 22 October 2009

FREiGEM



 FokA_pMA

back to index

FokA in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGA TGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTG ATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGAT GAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAAT TCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACC CGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGA GCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAG AAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL GGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNE WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTL TLEEVRRKFNNGEINFTG

    Commented GenBank file

  • FokA_pMA.txt



  •  Foki_pMA

    back to index

    Foki in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL GGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNE WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTL TLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Foki_pMA.txt



  •  Dig_pMA

    back to index

    Dig in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGCAGGTGGCCG CTTATCCCGACCACATCACCAAGTACGGCAAGTGCGGCTGGGCCGAGTAC ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTACAGCGTGATCCACGG CAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTGTGGGCGACAGCAAGA TCGGCAAGATCTACCACAGCTACACCATCGGCGGCGTGACCCAGGTGGGC GTGAGCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACTT TTGCAGATACGACGAGGACAAGAAGGGCCACTGGGACGCCGTGTGGGTGC TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA CTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCAACTGGTCCCACCCCC AGTTCGAAAAGACCGGTTAA

    AA sequence of ORF-1:

    MAGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGKSVKVS RYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIGYFCRY DEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNSNW SHPQFEKTG

    Commented GenBank file

  • Dig_pMA.txt



  •  FluA_pMA

    back to index

    FluA in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCA AGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTAC ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGG CAAAGAATACTTCATGGAAGGCACCGCCTACCCCGTGGGCGACAGCAAGA TCGGCAAGATCTACCACAGCCGGACCGTGGGCGGCTACACCAGAAAGACC GTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACAG CTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGC TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA CTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCGGTTAA

    AA sequence of ORF-1:

    MAGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGKSVKVS RYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIGYSCRY DEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNTG

    Commented GenBank file

  • FluA_pMA.txt



  •  DsbA_pMA

    back to index

    DsbA in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC ATCGGCGACCGGTTA

    AA sequence of ORF-1:

    MKKIWLALAGLVLAFSASATG

    Commented GenBank file

  • DsbA_pMA.txt



  •  StrepTag_pMA (iGEM team Freiburg 08)

    back to index

    StrepTag in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGTT

    AA sequence of ORF-1:

    MAGWSHPQFEKTG

    Commented GenBank file

  • StrepTag_pMA.txt



  •  HisTag_pMA (iGEM team Freiburg 08)

    back to index

    HisTag in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    GCCGGCCATCATCATCATCATCATACCGGT

    AA sequence of ORF-1:

    AGHHHHHHTG

    Commented GenBank file

  • HisTag_pMA.txt



  •  shortlinker26_pMA

    back to index

    shortlinker26 in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTACCGGT

    AA sequence of ORF-1:

    MAGGGSGTG

    Commented GenBank file

  • shortlinker26_pMA.txt



  •  Middellinker38_pMA

    back to index

    Middellinker38 in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGT

    AA sequence of ORF-1:

    MAGGGSGGGSGTG

    Commented GenBank file

  • Middellinker38_pMA.txt



  •  LongLinkerA50_pMA

    back to index

    LongLinkerA50 in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGT

    AA sequence of ORF-1:

    MAGGGSGGGSGTG

    Commented GenBank file

  • LongLinkerA50_pMA.txt



  •  GSAT_linker_pMA

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    GSAT_linker in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    GAGCTCGAATTCGCGGCCGCTTCTAGATGGCCGGCGGTGGTTCTGCCGGT GGCTCCGGTTCTGGCTCCAGCGGTGGCAGCTCTGGTGCGTCCGGCACGGG TACTGCGGGTGGCACTGGCAGCGGTTCCGGTACTGGCTCTGGCACCGGTA ATACTAGTAGCGGCCGCTGCAGGGTACC

    AA sequence of ORF-1:

    ELEFAAASRWPAVVLPVAPVLAPAVAALVRPARVLRVALAAVPVLALAPVILVAAAAGY

    Commented GenBank file

  • GSAT_linker_pMA.txt



  •  SEG_Linker_pMA

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    SEG_Linker in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    GAGCTCGAATTCGCGGCCGCTTCTAGATGGCCGGCGGTGGTTCTGGCGGC GGTTCTGAAGGTGGCGGCTCCGAAGGCGGCGGCAGCGAGGGCGGTGGTAG CGAAGGTGGTGGCTCCGAGGGTGGCGGTTCCGGCGGCGGTAGCACCGGTA ATACTAGTAGCGGCCGCTGCAGGGTACC

    AA sequence of ORF-1:

    ELEFAAASRWPAVVLAAVLKVAAPKAAAARAVVAKVVAPRVAVPAAVAPVILVAAAAGY

    Commented GenBank file

  • SEG_Linker_pMA.txt



  •  Split_Linker_pMA (iGEM team Freiburg 08)

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    Split_Linker in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    ATGGCCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGT TATGAATCACACCGGT

    AA sequence of ORF-1:

    MAGRPACKIPNDLKQKVMNHTG

    Commented GenBank file

  • Split_Linker_pMA.txt



  •  YFP(Venus)_pGA14 (iGEM team Freiburg 08)

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    YFP(Venus) in pGA14 Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    ATGGCCGGCATGAGCAAAGGCGAAGAACTGTTTACCGGCGTGGTGCCGAT TCTGGTGGAACTGGATGGTGATGTGAACGGCCATAAATTTAGCGTGAGCG GCGAAGGCGAAGGTGATGCGACCTATGGCAAACTGACCCTGAAACTGATT TGCACCACCGGCAAACTGCCGGTTCCGTGGCCGACCCTGGTTACCACCCT GGGCTATGGCCTGCAATGCTTTGCGCGTTATCCGGATCATATGAAACAGC ACGATTTCTTTAAAAGCGCCATGCCGGAAGGCTATGTGCAGGAACGCACC ATCTTTTTTAAAGATGATGGCAACTATAAAACCCGTGCGGAAGTGAAATT TGAAGGCGATACCCTGGTGAACCGTATTGAACTGAAAGGCATCGATTTCA AAGAAGATGGCAACATTCTGGGCCATAAACTGGAATATAACTACAACAGC CATAACGTGTATATCACCGCGGATAAACAGAAAAACGGCATCAAAGCGAA CTTTAAAATCCGCCACAACATTGAAGATGGCGGCGTGCAGCTGGCCGATC ATTATCAGCAGAACACCCCGATTGGTGATGGCCCGGTGCTGCTGCCGGAT AACCATTATCTGAGCTACCAGAGCAAACTGAGCAAAGATCCGAACGAAAA ACGTGACCATATGGTGCTGCTGGAATTTGTGACCGCGGCCGGTATTACCC ATGGCATGGATGAACTGTATAAAACCGGTTAA

    AA sequence of ORF-1:

    MAGMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPW PTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGD TLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQ LADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITHGMDELY KTG

    Commented GenBank file

  • YFP(Venus)_pGA14.txt



  •  Fos_pMA (iGEM team Freiburg 08)

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    Fos) in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    ATGGCCGGCCACCATCACCACCACCATGGATCCGGTGAAGAAAAACGCCG TATCCGTCGTGAGCGTAACAAAATGGCAGCAGCCAAATCCCGTAATCGCC GTCGTGAGCTGACAGACACACTGCAAGCCGAAACCGATCAACTGGAGGAC GAGAAAAGTGCTCTGCAAACTGAGATTGCCAATCTGCTGAAAGAGAAAGA AAAACTGGAGTTCATCCTGGCAGCAACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHGSGEEKRRIRRERNKMAAAKSRNRRRELTDTLQAETDQLEDEKSALQTEIA NLLKEKEKLEFILAATG

    Commented GenBank file

  • Fos_pMA.txt



  •  pJS418_Phagemid-dummy

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    pJS418 Vector with strong SD sequence

  • link to part registry
  • Commented GenBank file

  • pJS418.txt



  •  pJS419_Phagemid-dummy

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    pJS419 Vector with weak SD sequence

  • link to part registry
  • Commented GenBank file

  • pJS419.txt



  •  pBAD_dummy

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    pBAD Vector

  • link to part registry
  • Commented GenBank file

  • pBAD_dummy.txt



  •  pEX_CatInsert

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    pEX Vector

  • link to part registry
  • Commented GenBank file

  • pEX_CatInsert.txt