Team:Freiburg bioware/cloning1

From 2009.igem.org

(Difference between revisions)

Latest revision as of 03:38, 22 October 2009

FREiGEM

Index

*FokA_pMA
*Foki_pMA
*Dig_pMA
*FluA_pMA
*DsbA_pMA
*StrepTag_pMA
*HisTag_pMA
*shortlinker26_pMA
*Middellinker38_pMA
*LongLinkerA50_pMA
*GSAT_linker_pMA
*SEG_Linker_pMA
*Split_Linker_pMA
*YFP(Venus)_pGA14
*Fos_pMA
*pJS418 Vector
*pJS419 Vector
*pBAD Vector
*pEX Vector

list of oligonucleotides

Parts

FokA_pMA

back to index

FokA in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGA TGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTG ATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGAT GAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAAT TCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACC CGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGA GCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAG AAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

AA sequence of ORF-1:

MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL GGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNE WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTL TLEEVRRKFNNGEINFTG

Commented GenBank file

FokA_pMA.txt

Foki_pMA

back to index

Foki in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

AA sequence of ORF-1:

MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL GGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNE WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTL TLEEVRRKFNNGEINFTG

Commented GenBank file

Foki_pMA.txt

Dig_pMA

back to index

Dig in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGCAGGTGGCCG CTTATCCCGACCACATCACCAAGTACGGCAAGTGCGGCTGGGCCGAGTAC ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTACAGCGTGATCCACGG CAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTGTGGGCGACAGCAAGA TCGGCAAGATCTACCACAGCTACACCATCGGCGGCGTGACCCAGGTGGGC GTGAGCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACTT TTGCAGATACGACGAGGACAAGAAGGGCCACTGGGACGCCGTGTGGGTGC TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA CTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCAACTGGTCCCACCCCC AGTTCGAAAAGACCGGTTAA

AA sequence of ORF-1:

MAGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGKSVKVS RYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIGYFCRY DEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNSNW SHPQFEKTG

Commented GenBank file

Dig_pMA.txt

FluA_pMA

back to index

FluA in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCA AGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTAC ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGG CAAAGAATACTTCATGGAAGGCACCGCCTACCCCGTGGGCGACAGCAAGA TCGGCAAGATCTACCACAGCCGGACCGTGGGCGGCTACACCAGAAAGACC GTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACAG CTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGC TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA CTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCGGTTAA

AA sequence of ORF-1:

MAGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGKSVKVS RYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIGYSCRY DEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNTG

Commented GenBank file

FluA_pMA.txt

DsbA_pMA

back to index

DsbA in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC ATCGGCGACCGGTTA

AA sequence of ORF-1:

MKKIWLALAGLVLAFSASATG

Commented GenBank file

DsbA_pMA.txt

StrepTag_pMA (iGEM team Freiburg 08)

back to index

StrepTag in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGTT

AA sequence of ORF-1:

MAGWSHPQFEKTG

Commented GenBank file

StrepTag_pMA.txt

HisTag_pMA (iGEM team Freiburg 08)

back to index

HisTag in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
GCCGGCCATCATCATCATCATCATACCGGT

AA sequence of ORF-1:

AGHHHHHHTG

Commented GenBank file

HisTag_pMA.txt

shortlinker26_pMA

back to index

shortlinker26 in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
ATGGCCGGCGGTGGTTCTGGTACCGGT

AA sequence of ORF-1:

MAGGGSGTG

Commented GenBank file

shortlinker26_pMA.txt

Middellinker38_pMA

back to index

Middellinker38 in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGT

AA sequence of ORF-1:

MAGGGSGGGSGTG

Commented GenBank file

Middellinker38_pMA.txt

LongLinkerA50_pMA

back to index

LongLinkerA50 in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGT

AA sequence of ORF-1:

MAGGGSGGGSGTG

Commented GenBank file

LongLinkerA50_pMA.txt

GSAT_linker_pMA

back to index

GSAT_linker in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
GAGCTCGAATTCGCGGCCGCTTCTAGATGGCCGGCGGTGGTTCTGCCGGT GGCTCCGGTTCTGGCTCCAGCGGTGGCAGCTCTGGTGCGTCCGGCACGGG TACTGCGGGTGGCACTGGCAGCGGTTCCGGTACTGGCTCTGGCACCGGTA ATACTAGTAGCGGCCGCTGCAGGGTACC

AA sequence of ORF-1:

ELEFAAASRWPAVVLPVAPVLAPAVAALVRPARVLRVALAAVPVLALAPVILVAAAAGY

Commented GenBank file

GSAT_linker_pMA.txt

SEG_Linker_pMA

back to index

SEG_Linker in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
GAGCTCGAATTCGCGGCCGCTTCTAGATGGCCGGCGGTGGTTCTGGCGGC GGTTCTGAAGGTGGCGGCTCCGAAGGCGGCGGCAGCGAGGGCGGTGGTAG CGAAGGTGGTGGCTCCGAGGGTGGCGGTTCCGGCGGCGGTAGCACCGGTA ATACTAGTAGCGGCCGCTGCAGGGTACC

AA sequence of ORF-1:

ELEFAAASRWPAVVLAAVLKVAAPKAAAARAVVAKVVAPRVAVPAAVAPVILVAAAAGY

Commented GenBank file

SEG_Linker_pMA.txt

Split_Linker_pMA (iGEM team Freiburg 08)

back to index

Split_Linker in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
ATGGCCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGT TATGAATCACACCGGT

AA sequence of ORF-1:

MAGRPACKIPNDLKQKVMNHTG

Commented GenBank file

Split_Linker_pMA.txt

YFP(Venus)_pGA14 (iGEM team Freiburg 08)

back to index

YFP(Venus) in pGA14 Vector

link to part registry

nucleotid sequence of ORF-1:
ATGGCCGGCATGAGCAAAGGCGAAGAACTGTTTACCGGCGTGGTGCCGAT TCTGGTGGAACTGGATGGTGATGTGAACGGCCATAAATTTAGCGTGAGCG GCGAAGGCGAAGGTGATGCGACCTATGGCAAACTGACCCTGAAACTGATT TGCACCACCGGCAAACTGCCGGTTCCGTGGCCGACCCTGGTTACCACCCT GGGCTATGGCCTGCAATGCTTTGCGCGTTATCCGGATCATATGAAACAGC ACGATTTCTTTAAAAGCGCCATGCCGGAAGGCTATGTGCAGGAACGCACC ATCTTTTTTAAAGATGATGGCAACTATAAAACCCGTGCGGAAGTGAAATT TGAAGGCGATACCCTGGTGAACCGTATTGAACTGAAAGGCATCGATTTCA AAGAAGATGGCAACATTCTGGGCCATAAACTGGAATATAACTACAACAGC CATAACGTGTATATCACCGCGGATAAACAGAAAAACGGCATCAAAGCGAA CTTTAAAATCCGCCACAACATTGAAGATGGCGGCGTGCAGCTGGCCGATC ATTATCAGCAGAACACCCCGATTGGTGATGGCCCGGTGCTGCTGCCGGAT AACCATTATCTGAGCTACCAGAGCAAACTGAGCAAAGATCCGAACGAAAA ACGTGACCATATGGTGCTGCTGGAATTTGTGACCGCGGCCGGTATTACCC ATGGCATGGATGAACTGTATAAAACCGGTTAA

AA sequence of ORF-1:

MAGMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPW PTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGD TLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQ LADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITHGMDELY KTG

Commented GenBank file

YFP(Venus)_pGA14.txt

Fos_pMA (iGEM team Freiburg 08)

back to index

Fos) in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
ATGGCCGGCCACCATCACCACCACCATGGATCCGGTGAAGAAAAACGCCG TATCCGTCGTGAGCGTAACAAAATGGCAGCAGCCAAATCCCGTAATCGCC GTCGTGAGCTGACAGACACACTGCAAGCCGAAACCGATCAACTGGAGGAC GAGAAAAGTGCTCTGCAAACTGAGATTGCCAATCTGCTGAAAGAGAAAGA AAAACTGGAGTTCATCCTGGCAGCAACCGGTTAA

AA sequence of ORF-1:

MAGHHHHHHGSGEEKRRIRRERNKMAAAKSRNRRRELTDTLQAETDQLEDEKSALQTEIA NLLKEKEKLEFILAATG

Commented GenBank file

Fos_pMA.txt

pJS418_Phagemid-dummy

back to index

pJS418 Vector with strong SD sequence

link to part registry

Commented GenBank file

pJS418.txt

pJS419_Phagemid-dummy

back to index

pJS419 Vector with weak SD sequence

link to part registry

Commented GenBank file

pJS419.txt

pBAD_dummy

back to index

pBAD Vector

link to part registry

Commented GenBank file

pBAD_dummy.txt

pEX_CatInsert

back to index

pEX Vector

link to part registry

Commented GenBank file

pEX_CatInsert.txt

@@ Line 48: / Line 48: @@
   href="https://2009.igem.org/Team:Freiburg_bioware/Project#Summary">Summary</a>
        </li>
-       <li><a
- href="https://2009.igem.org/Team:Freiburg_bioware/Project#Subprojects">Subprojects</a></li>
        <li><a
   href="https://2009.igem.org/Team:Freiburg_bioware/Project#Highlights">Highlights</a></li>
@@ Line 58: / Line 57: @@
   class="t">Human
 Practice</span></a>
+        <ul>
+      <li><a
+ href="https://2009.igem.org/Team:Freiburg_bioware/Human_Practice/Ethics">Ethics</a>
+      </li>
+      <li><a
+ href="https://2009.igem.org/Team:Freiburg_bioware/Human_Practice/Safety">Safety</a></li>
+     </ul>
    </li>
    <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Notebook"><span class="l"></span><span
@@ Line 77: / Line 83: @@
   class="l"></span><span class="r"></span><span
   class="t">Collaboration</span></a></li>
+<li><a
+ href="https://2009.igem.org/Team:Freiburg_bioware/Modeling"><span
+ class="l"></span><span class="r"></span><span
+ class="t">Modeling</span></a></li>
 </ul>
 </div>
@@ Line 123: / Line 133: @@
 </a><br>
 *<a href="#11">LongLinkerA50_pMA
+</a><br>
+*<a href="#12">GSAT_linker_pMA
+</a><br>
+*<a href="#13">SEG_Linker_pMA
+</a><br>
+*<a href="#14">Split_Linker_pMA
+</a><br>
+*<a href="#15">YFP(Venus)_pGA14
+</a><br>
+*<a href="#19">Fos_pMA
+</a><br>
+*<a href="#16">pJS418 Vector
+</a><br>
+*<a href="#17">pJS419 Vector
+</a><br>
+*<a href="#18">pBAD Vector
+</a><br>
+*<a href="#20">pEX Vector
 </a><br>
@@ Line 128: / Line 156: @@
 </td></tr></table>
+<li style=" text-align:center;"><a href="https://2009.igem.org/Team:Freiburg_bioware/oligos">list of oligonucleotides</a></li>
@@ Line 640: / Line 668: @@
-<a name="12"></a>
+<a name="13"></a>
 <h3 class="art-PostHeaderIcon-wrapper"> &nbsp;SEG_Linker_pMA <span class="art-PostHeader"></span> </h3>
 <div class="art-PostContent">
@@ Line 678: / Line 706: @@
-<a name="12"></a>
+<a name="14"></a>
 <h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Split_Linker_pMA (iGEM team Freiburg 08)<span class="art-PostHeader"></span> </h3>
 <div class="art-PostContent">
@@ Line 713: / Line 741: @@
-<a name="12"></a>
+<a name="15"></a>
 <h3 class="art-PostHeaderIcon-wrapper"> &nbsp;YFP(Venus)_pGA14 (iGEM team Freiburg 08) <span class="art-PostHeader"></span> </h3>
 <div class="art-PostContent">
@@ Line 766: / Line 794: @@
+<a name="19"></a>
+<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Fos_pMA (iGEM team Freiburg 08) <span class="art-PostHeader"></span> </h3>
+<div class="art-PostContent">
+<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-<a name="12"></a>
+<table border="5" style= "width: 100%;">
+<tr>
+<td align="center">
+<img src="https://static.igem.org/mediawiki/2009/thumb/c/ca/Freiburg09_Fos_pMA.jpg/600px-Freiburg09_Fos_pMA.jpg" name="Fos in pMA Vector" width="480" height="480" />
+<br><p align="center">Fos) in pMA Vector</p>
+<li> <a href="http://partsregistry.org/Part:BBa_K243027">link to part registry</a>
+</td>
+<td align="left">
+nucleotid sequence of ORF-1:<br>
+ATGGCCGGCCACCATCACCACCACCATGGATCCGGTGAAGAAAAACGCCG
+TATCCGTCGTGAGCGTAACAAAATGGCAGCAGCCAAATCCCGTAATCGCC
+GTCGTGAGCTGACAGACACACTGCAAGCCGAAACCGATCAACTGGAGGAC
+GAGAAAAGTGCTCTGCAAACTGAGATTGCCAATCTGCTGAAAGAGAAAGA
+AAAACTGGAGTTCATCCTGGCAGCAACCGGTTAA
+</td>
+</tr>
+</table>
+<br>
+<h4>AA sequence of ORF-1:<br></h4>
+<table border="0">
+<p align="justify"><td align="left">
+MAGHHHHHHGSGEEKRRIRRERNKMAAAKSRNRRRELTDTLQAETDQLEDEKSALQTEIA
+NLLKEKEKLEFILAATG
+<br><br></p></td></table>
+<h4>Commented GenBank file</h4>
+<li> <a href="https://static.igem.org/mediawiki/2009/8/80/Freiburg09_Fos_pMA.txt">Fos_pMA.txt</a></li>
+<br>
+<br>
+<br>
+<a name="16"></a>
 <h3 class="art-PostHeaderIcon-wrapper"> &nbsp;pJS418_Phagemid-dummy <span class="art-PostHeader"></span> </h3>
 <div class="art-PostContent">
@@ Line 786: / Line 861: @@
 </tr>
 </table>
 <br>
 <br>
@@ Line 792: / Line 866: @@
-<a name="12"></a>
+<a name="17"></a>
 <h3 class="art-PostHeaderIcon-wrapper"> &nbsp;pJS419_Phagemid-dummy <span class="art-PostHeader"></span> </h3>
 <div class="art-PostContent">
@@ Line 802: / Line 876: @@
 <img src="https://static.igem.org/mediawiki/2009/thumb/b/b1/Freiburg09_PJS419_dummy.jpg/600px-Freiburg09_PJS419_dummy.jpg" name="pJS419 Vector with weak SD sequence" width="480" height="480" />
 <br><p align="center">pJS419 Vector with weak SD sequence</p>
-<li> <a href="http://partsregistry.org/Part:BBa_K243034">link to part registry</a>
+<li> <a href="http://partsregistry.org/Part:BBa_K243035">link to part registry</a>
 </td>
 <td align="center">
@@ Line 818: / Line 892: @@
+<a name="18"></a>
+<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;pBAD_dummy <span class="art-PostHeader"></span> </h3>
+<div class="art-PostContent">
+<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
+<table border="5" style= "width: 100%;">
+<tr>
+<td align="center">
+<img src="https://static.igem.org/mediawiki/2009/thumb/6/69/Freiburg09_PBAD_dummy.jpg/600px-Freiburg09_PBAD_dummy.jpg" name="pBAD Vector" width="480" height="480" />
+<br><p align="center">pBAD Vector</p>
+<li> <a href="http://partsregistry.org/Part:BBa_K243037">link to part registry</a>
+</td>
+<td align="center">
+<h4>Commented GenBank file</h4>
+<li> <a href="https://static.igem.org/mediawiki/2009/1/1c/Freiburg09_PBAD_dummy.txt">pBAD_dummy.txt</a></li>
+</td>
+</tr>
+</table>
+<br>
+<br>
+<br>
+<a name="20"></a>
+<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;pEX_CatInsert <span class="art-PostHeader"></span> </h3>
+<div class="art-PostContent">
+<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
+<table border="5" style= "width: 100%;">
+<tr>
+<td align="center">
+<img src="https://static.igem.org/mediawiki/2009/thumb/a/a6/Freiburg09_PEX_CATInsert.jpg/600px-Freiburg09_PEX_CATInsert.jpg" name="pEX Vector" width="480" height="480" />
+<br><p align="center">pEX Vector</p>
+<li> <a href="http://partsregistry.org/Part:BBa_K243033">link to part registry</a>
+</td>
+<td align="center">
+<h4>Commented GenBank file</h4>
+<li> <a href="https://static.igem.org/mediawiki/2009/7/7a/Freiburg09_PEX_CATInsert.txt">pEX_CatInsert.txt</a></li>
+</td>
+</tr>
+</table>
+<br>
+<br>
+<br>