Team:Groningen/Alignment

From 2009.igem.org

(Difference between revisions)

Revision as of 12:34, 3 August 2009

The theoretical modeling data is used from papers is based on plasmids R46 and R773. To see the different between the chromosomal and the plasmids genes, they are alignment with clustalW. The functional binding site from arsenic is on residues Cys32, Cys34, and the nonessential Cys37. Plasmids R46, R773, strain K12 and DH10B are alignment, and all have the same functional binding site on the same position. The distance between the genes is not significant. We assumption that there no difference in binding in arsenic and function between chromosomal and plasmids genes.

Alignment with CLUSTALW

Phylogram with CLUSTALW

@@ Line 1: / Line 1: @@
-The theoretical  modeling data is used from papers is based on plasmids R46 and R773. To see the different between the chromosomal and the plasmids genes, they are alignment with clustalW. The functional binding site from arsenic is on residues Cys32, Cys34, and the nonessential Cys37 (REF). Plasmids R46, R773, strain K12 and DH10B have all the same functional binding site on the same position.
+The theoretical  modeling data is used from papers is based on plasmids R46 and R773. To see the different between the chromosomal and the plasmids genes, they are alignment with clustalW. The functional binding site from arsenic is on residues Cys32, Cys34, and the nonessential Cys37. Plasmids R46, R773, strain K12 and DH10B are alignment, and all have the same functional binding site on the same position. The distance between the genes is not significant. We assumption that there no difference in binding in arsenic and function between chromosomal and plasmids genes.
 [[Image:Alignment.PNG|center|900px|]]
+Alignment with CLUSTALW
+[[Image:Phylogram.PNG|center|900px|]]
+Phylogram with CLUSTALW