"
Team:Groningen/Alignment
From 2009.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | The theoretical modeling data is used from papers is based on plasmids R46 and R773. To see the different between the chromosomal and the plasmids genes, they are alignment with clustalW. The functional binding site from arsenic is on residues Cys32, Cys34, and the nonessential Cys37 ([[Team:Groningen/Literature#Rensing2005|Rensing2005]]. Plasmids R46, R773, strain K12 and DH10B are alignment, and all have the same functional binding site on the same position. The distance between the genes is not significant. | + | The theoretical modeling data is used from papers is based on plasmids R46 and R773. To see the different between the chromosomal and the plasmids genes, they are alignment with [http://www.ebi.ac.uk/Tools/clustalw2/index.html clustalW]. The functional binding site from arsenic is on residues Cys32, Cys34, and the nonessential Cys37 ([[Team:Groningen/Literature#Rensing2005|Rensing2005]]). Plasmids R46, R773, strain K12 and DH10B are alignment, and all have the same functional binding site on the same position. The distance between the genes is not significant. The assumption is that there no difference in binding in arsenic and function between chromosomal and plasmids genes. |
Revision as of 12:51, 3 August 2009
The theoretical modeling data is used from papers is based on plasmids R46 and R773. To see the different between the chromosomal and the plasmids genes, they are alignment with clustalW. The functional binding site from arsenic is on residues Cys32, Cys34, and the nonessential Cys37 (Rensing2005). Plasmids R46, R773, strain K12 and DH10B are alignment, and all have the same functional binding site on the same position. The distance between the genes is not significant. The assumption is that there no difference in binding in arsenic and function between chromosomal and plasmids genes.
Alignment with CLUSTALW
Phylogram with CLUSTALW
