Team:Groningen/Brainstorm/Growth Control
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Introduction
Bacteria have a choice between using nutrients for growth or for the production of (commercially) valuable proteins. Being able to control the bacterial growth cycle and inducing a premature stationary phase will create the possibility to spend more time producing, using less nutrients for biomass and more for desired production. Stationary phase is nothing more than a stop in an increase of cell numbers by cell death being equal to cell growth. Normally this is induced by the limitation of available nutrients or by means of quorum sensing in response to high cell density. With this knowledge, a culture can be created that can respond by limited cell death in response to an added molecule that mimics the response to high cell density.
See also Chemostat
One of the most important features of chemostats is that micro-organisms can be grown in a physiological steady state. In steady state, all culture parameters remain constant (culture volume, dissolved oxygen concentration, nutrient and product concentrations, pH, cell density, etc.). Because obtaining a steady state requires at least 5 volume changes, chemostats require large nutrient and waste reservoirs. Creating biological "chemostat" would circumvent these drawbacks.
Previous contests
Quorum Sensing
Cell cycle
Parts in the Registry of Standard Parts:
Quorum sensing
- BBa_K104001: Sensor for small peptide Subtilin
- BBa_I13211: Biobricked version of the natural Lux quorum sensing system
Cell cycle
- BBa_M31201
- BBa_K105013
- BBa_K105015
- BBa_K101017: a cell-cycle dependent promoter that is repressed before initiation of replication and depressed shortly after
BBa_J22051: an adenylate cyclase promoter, expression is repressed during cell division- BBa_J22052: an adenylate cyclase promoter, expression is repressed during cell division
- BBa_J22095
- BBa_J22092
- BBa_K142040: Ribosome modulation factor (RMF)
- BBa_K142041: Arabinose controlled RMF generator
Cell death
- BBa_I745006
- BBa_I745007
- BBa_K145008: LuxR Generator
- BBa_K145009: ccdB cell death gene under control of an activating Lux PR
- BBa_K145109: ccdB cell death gene under the control of a hybrid LuxPR P22 C2 promotor
- BBa_K145110: Complete cell death mechanism. Combination of BBa_K145108 and Part:BBa_K145109
- BBa_K145151: Coding region for the ccdB (control of cell death) gene
- BBa_K145230: A hybrid promoter controls the production of LuxR and ccdB
- BBa_K145256: Cell death Part 1
- BBa_K145257: Cell death Part 2
- BBa_K124003: Induces lysis in E. Coli bacteria
- BBa_K124014: Induces lysis faster in E. Coli bacteria
- BBa_K124017: Complete casette containing BBa_K124014
Related Literature
Quorum sensing
- Quorum sensing control of lantibiotic production; nisin and subtilin autoregulate their own biosynthesis, Kleerebezem
- In this paper, the molecular mechanism underlying regulation of nisin and subtilin production is reviewed.
- Induction of entry into the stationary growth phase in Pseudomonas aeruginosa by N-acylhomoserine lactone, You et al.
- Addition of N-acylhomoserine lactone in the exponential growth phase, regardless of cell density, induces a repression of cell growth of P. aeruginosa
- Induction of natural competence in Streptococcus pneumoniae triggers lysis and DNA release from a subfraction of the cell population, Steinmoen et al.
- In this study Competence stimulating peptide is shown to initiates release of DNA from a subfraction of the bacterial population, probably by cell lysis.
- A synthetic Escherichia coli predator–prey ecosystem, Balagaddé et al.
- In this study they created a synthetic ecosystem with bi-directional communication through quorum sensing which regulate each other's gene expression and survival via engineered gene circuits.