Team:Groningen/Notebook/12 August 2009
From 2009.igem.org
Wet
GVP Cluster
TODO isolate plasmids from o.n. cultures
TODO run gel to check plasmid size (should be ~9000bp)
Transporters
Metal Accumulation
MBP-ArsR fusion protein ligation was transformed for a second try, plated out on LB-amp100 (50 μL and concentrated after centrifugation) Positive control (pSB1AC3-high constitutive promotor) ∞ Negative control (pSB1AC3-ccdb)1 colony on the concentrated plate Ligations had no colonies.
Vectors
Dry
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