Team:Groningen/Notebook/15 August 2009

From 2009.igem.org

(Difference between revisions)
(Floating bacteria!)
 
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==Wet==
==Wet==
-
 
+
Some plates were found in the incubator again, Frans'? AC3 plated out 13/8/9 had one colony, put to fridge.
===GVP Cluster===
===GVP Cluster===
<center>[[Image:15august2009_Float_test_with_GVP_plasmid.jpg]]</center>
<center>[[Image:15august2009_Float_test_with_GVP_plasmid.jpg]]</center>
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===Metal Accumulation===
===Metal Accumulation===
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+
MymT transformations
 +
All below had colonies in the morning (plated out on LB<sub>Amp</sub> 14august2009)
 +
*<i>E. coli</i> TOP10 with pSB1AC3-MymT 1(6x) [GNK conc.]
 +
*<i>E. coli</i> TOP10 with pSB1AC3-MymT 2(6x) [GNK conc.]
 +
*<i>E. coli</i> TOP10 with pSB1AC3-MymT 1(6x) [GNK conc.] (more colonies than the one above)
 +
Below had colonies at the end of the day
 +
*<i>E. coli</i> TOP10 with pSB1AC3-MymT 1(3x) [GNK conc.]
 +
*<i>E. coli</i> TOP10 with pSB1AC3-MymT 2(3x) [GNK conc.]
 +
*<i>E. coli</i> TOP10 with pSB1AC3-MymT 1(6x) [GNK]
 +
Codes were copied from plates and not interpreted. All plates with colonies were put to fridge, all without were put back to 37 °C.
===Vectors===
===Vectors===

Latest revision as of 14:05, 15 August 2009

Igemhomelogo.png

Wet

Some plates were found in the incubator again, Frans'? AC3 plated out 13/8/9 had one colony, put to fridge.

GVP Cluster

15august2009 Float test with GVP plasmid.jpg

E. coli TOP10 cells grown on plate with IPTG and resuspended in saline solution (0.9% NaCl). Left is control, no Gas Vesicle Proteins (GVP), Right is with IPTG induced GVP. Picture is after one day.

Transporters

Metal Accumulation

MymT transformations All below had colonies in the morning (plated out on LBAmp 14august2009)

  • E. coli TOP10 with pSB1AC3-MymT 1(6x) [GNK conc.]
  • E. coli TOP10 with pSB1AC3-MymT 2(6x) [GNK conc.]
  • E. coli TOP10 with pSB1AC3-MymT 1(6x) [GNK conc.] (more colonies than the one above)

Below had colonies at the end of the day

  • E. coli TOP10 with pSB1AC3-MymT 1(3x) [GNK conc.]
  • E. coli TOP10 with pSB1AC3-MymT 2(3x) [GNK conc.]
  • E. coli TOP10 with pSB1AC3-MymT 1(6x) [GNK]

Codes were copied from plates and not interpreted. All plates with colonies were put to fridge, all without were put back to 37 °C.

Vectors

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30