http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_August_2009&feed=atom&action=history
Team:Groningen/Notebook/15 August 2009 - Revision history
2024-03-28T13:15:02Z
Revision history for this page on the wiki
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http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_August_2009&diff=47083&oldid=prev
Wilfred at 14:05, 15 August 2009
2009-08-15T14:05:43Z
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 14:05, 15 August 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Wet==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Wet==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Some plates were found in the incubator again, Frans'? AC3 plated out 13/8/9 had one colony, put to fridge.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===GVP Cluster===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===GVP Cluster===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center>[[Image:15august2009_Float_test_with_GVP_plasmid.jpg]]</center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center>[[Image:15august2009_Float_test_with_GVP_plasmid.jpg]]</center></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Metal Accumulation===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Metal Accumulation===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">MymT transformations</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">All below had colonies in the morning (plated out on LB<sub>Amp</sub> 14august2009)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*<i>E. coli</i> TOP10 with pSB1AC3-MymT 1(6x) [GNK conc.]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*<i>E. coli</i> TOP10 with pSB1AC3-MymT 2(6x) [GNK conc.]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*<i>E. coli</i> TOP10 with pSB1AC3-MymT 1(6x) [GNK conc.] (more colonies than the one above)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Below had colonies at the end of the day</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*<i>E. coli</i> TOP10 with pSB1AC3-MymT 1(3x) [GNK conc.]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*<i>E. coli</i> TOP10 with pSB1AC3-MymT 2(3x) [GNK conc.]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*<i>E. coli</i> TOP10 with pSB1AC3-MymT 1(6x) [GNK]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Codes were copied from plates and not interpreted. All plates with colonies were put to fridge, all without were put back to 37 °C.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Vectors===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Vectors===</div></td></tr>
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Wilfred
http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_August_2009&diff=47062&oldid=prev
Wilfred: Floating bacteria!
2009-08-15T10:30:51Z
<p>Floating bacteria!</p>
<p><b>New page</b></p><div>{{Team:Groningen/Notebook/Day/Header}}<br />
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==Wet==<br />
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===GVP Cluster===<br />
<center>[[Image:15august2009_Float_test_with_GVP_plasmid.jpg]]</center><br />
<i>E. coli</i> TOP10 cells grown on plate with IPTG and resuspended in saline solution (0.9% NaCl). <br />
Left is control, no Gas Vesicle Proteins (GVP), Right is with IPTG induced GVP. Picture is after one day.<br />
===Transporters===<br />
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===Metal Accumulation===<br />
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===Vectors===<br />
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==Dry==<br />
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{{Team:Groningen/Notebook/Day/Footer}}</div>
Wilfred