Team:Groningen/Notebook/18 September 2009

From 2009.igem.org

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Wet

GVP Cluster

Planning

TODO work out the wiki page for GVP
  • made a layout
  • still have to read all articles
  • modeling will stay as it is, has been done by modeling people
DONE make a doodle for presentation planning (1-19 oct.)
DONE media attention
  • mail to UK, Ing., St. Gen.
  • facebook account with link to twitter
  • ethics survey link on facebook and twitter
DONE place an ethics survey link on twitter


DONE clone pArsR-GVP into pSB2K3
  • TODO still need to make glycerol stock
TODO clone repeat out of GVP cluster
TODO make glycerol stocks of constructs
TODO enter info on part registry


Plates

Name Plasmid Used Antibiotics on Plasmid No. of Colonies Date
Positive (high) J61002 (with J23101) Ampicillin ~1500 (red) 17 sept.
Negative (high) None None 8 (white/yellow) 17 sept.
pMA-gvpL (low) pMA Ampicillin ~150 17 sept.
pMA-gvpL (high) pMA Ampicillin ~650 17 sept.
pMA-GlpF (low) pMA Ampicillin ~125 17 sept.
pMA-gvpL (high) pMA Ampicillin ~550 17 sept.
pNL29 (SJ)(low) pBlueScript_II_KS_(+) Ampicillin ~100 17 sept.
pNL29 (high) pBlueScript_II_KS_(+) Ampicillin ~250 17 sept.
pArsR-GVP (on Ampicillin) pSB2K3 Kanamycin 0 17 sept.
pArsR-GVP (Glycerol Stock) pSB2K3 Kanamycin Single Colonies 17 sept.
pArsR-GVP (Saline Test) pSB2K3 Kanamycin Plate Full-Grown 17 sept.
pZntR-GVP (Saline Test) pSB2K3 Kanamycin Plate Full-Grown 17 sept.
pCueO-GVP (Saline Test) pSB2K3 Kanamycin Plate Full-Grown 17 sept.


→ The plates showed the expected amount of colonies, and also red in the case of the positive plates. The eight colonies on the negative plate can be taken into acount when plating transformed cells.


→ The plate with stripes of pArsR-GVP (pSB2K3) preculture showed growth and some single colonies.


→ All plates were stored in the fridge for further use on monday.


Cultures

The overnight cultures with LB-amp100 medium of colonies E.coli TOP10 with pNL29 in pBlueScript_II_KS_(+) showed no growth.


→ The plate used for culture inoculation was very (more than a month) old, and was probably no good anymore. The new transformed E.coli TOP10 with original pNL29 plasmid will replace all excisting stocks/plates.

Transporters

Metal Accumulation

Vectors

Dry

April
MTWTFSS
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6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
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4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
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6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
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7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30