Team:Groningen/Notebook/24 August 2009

From 2009.igem.org

(Difference between revisions)
(Vectors)
(Metal promotors)
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===Vectors===
===Vectors===
====Metal promotors====
====Metal promotors====
-
Protocol #3
+
[http://wolfson.huji.ac.il/expression/vector/origin.html#top origin of replication] determines:
-
* Phosphorylation and annealing synthetic oligo's
+
* Vector copy number
-
* Ligation in pSB1AC3
+
* Plasmid compatibility: its ability to replicate in conjuction with another plasmid.
 +
** Plasmids that utilize the same replication system cannot co-exist in the same bacterial cell.
 +
** pUC19 and ColE1 are in different [http://wolfson.huji.ac.il/expression/vector/origin.html#comp compatibility group]
==Dry==
==Dry==
{{Team:Groningen/Notebook/Day/Footer}}
{{Team:Groningen/Notebook/Day/Footer}}

Revision as of 13:26, 24 August 2009

Igemhomelogo.png

Wet

GVP Cluster

TODO Restriction of GVP and pSB1AC3-Lac/pBAD for assembly
TODO Gel purification of wanted fragments and nanodrop
TODO Clone GVP cluster behind Lac and Arabinose promoter for expression tests in pSB1AC3 plasmid
TODO Send plasmids with MEtal promoters in BBa_J61002 vector for sequencing
TODO Check MEtal + RBS promoters on gel with restriction (also XbaI/PstI in Zinc promoter)
TODO Test promoter strenght compared to BBa_J23101 promoter (Sven)
TODO Enter sequences of constructs to Sandbox

Transporters

Metal Accumulation

Vectors

Metal promotors

origin of replication determines:

  • Vector copy number
  • Plasmid compatibility: its ability to replicate in conjuction with another plasmid.
    • Plasmids that utilize the same replication system cannot co-exist in the same bacterial cell.
    • pUC19 and ColE1 are in different compatibility group

Dry

April
MTWTFSS
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6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
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4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
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6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
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3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
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7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30