Team:Groningen/Notebook/25 August 2009
From 2009.igem.org
(Difference between revisions)
m (→Metal promotors) |
(→GVP Cluster) |
||
Line 4: | Line 4: | ||
===GVP Cluster=== | ===GVP Cluster=== | ||
+ | |||
+ | :→ {{todo}} Restriction of GVP and pSB1AC3-Lac/pBAD for assembly | ||
+ | :→ {{todo}} Gel purification of wanted fragments and nanodrop | ||
+ | :→ {{todo}} Clone GVP cluster behind Lac and Arabinose promoter for expression tests in pSB1AC3 plasmid | ||
+ | :→ {{todo}} Send plasmids with ME<sup>tal</sup> promoters in BBa_J61002 vector for sequencing | ||
+ | :→ {{todo}} Check ME<sup>tal</sup> + RBS promoters on gel with restriction (also XbaI/PstI in Zinc promoter) | ||
+ | |||
+ | :→ {{todo}} Test promoter strenght compared to BBa_J23101 promoter (Sven) | ||
+ | :→ {{todo}} Enter sequences of constructs to Sandbox | ||
===Transporters=== | ===Transporters=== |
Revision as of 14:46, 24 August 2009
Wet
GVP Cluster
- → TODO Restriction of GVP and pSB1AC3-Lac/pBAD for assembly
- → TODO Gel purification of wanted fragments and nanodrop
- → TODO Clone GVP cluster behind Lac and Arabinose promoter for expression tests in pSB1AC3 plasmid
- → TODO Send plasmids with MEtal promoters in BBa_J61002 vector for sequencing
- → TODO Check MEtal + RBS promoters on gel with restriction (also XbaI/PstI in Zinc promoter)
- → TODO Test promoter strenght compared to BBa_J23101 promoter (Sven)
- → TODO Enter sequences of constructs to Sandbox
Transporters
Metal Accumulation
Vectors
Metal promotors
Cloning into pSB1AC3 and pSB3K3 has stopped
Dry
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
|