From 2009.igem.org
Wet
GVP Cluster
- → TODO Restriction of GVP and pSB1AC3-Lac/pBAD for assembly
- → TODO Gel purification of wanted fragments and nanodrop
- → TODO Clone GVP cluster behind Lac and Arabinose promoter for expression tests in pSB1AC3 plasmid
- → DONE Send plasmids with MEtal promoters in BBa_J61002 vector for sequencing
- → DONE Check MEtal + RBS promoters on gel with restriction (also XbaI/PstI in Zinc promoter)
- → TODO Test promoter strenght compared to BBa_J23101 promoter (Sven)
- → DONE Enter sequences of constructs to Sandbox
Transporters
Metal Accumulation
PCR check of MPB-ArsR fusion protein, two combinations of primers were used; Forward MBP with Reverse ArsR & Forward ArsR with Reverse MBP, giving the indication where proteins are in comparison of each other. Only the first gave the expected product of 1500 bp, indicating that the fusion was as expected MBP-ArsR and nog ArsR-MBP. Sample was send for sequencing.
Vectors
Metal promotors
Cloning into pSB1AC3 and pSB3K3 has stopped
Dry
Jasper finally finished simplifying the differential equations and they are now presented in neat tables on our modelling page. It turned out that it is easiest to work with differential equations for the totals of substances in combination with relative abundances of the different forms of the substances.
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