Team:Groningen/Notebook/27 August 2009
From 2009.igem.org
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===Transporters=== | ===Transporters=== |
Revision as of 07:46, 27 August 2009
Wet
GVP Cluster
- → TODO Check o.n. plates with transformed E.coli TOP10 cells
- → TODO Use colonies to grow o.n. precultures for plasmid isolation
- → TODO Test promoter strenght compared to BBa_J23101 promoter (Sven)
- → TODO Enter sequences of constructs to Sandbox
Colonies on Plates
Name | Plasmid Used | Antibiotics on Plasmid | No. of Colonies | Date |
LAC-GVP (new) | pSB1AC3 | Ampicillin | ~80 | 27/8 |
LAC-GVP (new, concentrated) | pSB1AC3 | Ampicillin | ~200 | 27/8 |
LAC-GVP (old) | pSB1AC3 | Ampicillin | 3 | 27/8 |
LAC-GVP (old, concentrated) | pSB1AC3 | Ampicillin | 11 | 27/8 |
pBAD-GVP (new) | pSB1AC3 | Ampicillin | 18 | 27/8 |
pBAD-GVP (new, concentrated) | pSB1AC3 | Ampicillin | ~80 | 27/8 |
pBAD-GVP (old) | pSB1AC3 | Ampicillin | 4 | 27/8 |
pBAD-GVP (old, concentrated) | pSB1AC3 | Ampicillin | 28 | 27/8 |
pLow (constitutive)-GVP (old) | pSB3K3 | Kanamycin | ~80 | 27/8 |
pLow (constitutive)-GVP (old, concentrated) | pSB3K3 | Kanamycin | ~80 | 27/8 |
MQ (neg. control) | X | X | 0 | 27/8 |
pHigh (constitutive)-RFP (pos. control) | J61002 | Ampicillin | ~2000 | 27/8 |
Transporters
Metal Accumulation
Vectors
Dry
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