Team:Groningen/Notebook/28 July 2009

From 2009.igem.org

(Difference between revisions)
(Transporters)
(Transporters)
Line 6: Line 6:
===Transporters===
===Transporters===
 +
Result of GlpF PCR3 done yesterday.
 +
[[Image:GlpFPCR3_noted.JPG]]
[[Image:GlpFPCR3_noted.JPG]]
 +
 +
a band of 903 was seen as expected. The bands were cut out of the gel and  gel extraction was done using ..kit.
===Metal Accumulation===
===Metal Accumulation===

Revision as of 13:56, 28 July 2009

Igemhomelogo.png

Wet

GVP Cluster

Transporters

Result of GlpF PCR3 done yesterday.

GlpFPCR3 noted.JPG

a band of 903 was seen as expected. The bands were cut out of the gel and gel extraction was done using ..kit.

Metal Accumulation

Vectors

Dry

Jasper started reading about SmtA, but so far no luck in finding any quantitative results that can be used for modelling. At least, it seems that way. The main things that can be found that might be useful are the amount of metal bound to SmtA in Shi1992, a comparison of zinc accumulation with and without smt(A) in Turner1995 (not in E. coli) and PMPS-titration/H+-competition curves in Shi1992 and Blindauer2002. However, it is not yet clear if (and how) this data can be used to derive useful parameters for modelling.


April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30