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Team:Groningen/Notebook/30 August 2009
From 2009.igem.org
(Difference between revisions)
m (New page: {{Team:Groningen/Notebook/Day/Header}} =Weekend stuff= ==Transformation== ==Liquid cultures== {{Team:Groningen/Notebook/Day/Footer}}) |
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=Weekend stuff= | =Weekend stuff= | ||
| + | ==Metal accumulation== | ||
==Transformation== | ==Transformation== | ||
| - | = | + | {| border="1" |
| + | |+'''SmtA+promotor''' | ||
| + | !<u>Colonies</u> | ||
| + | !<u>Name</u> | ||
| + | !<u>Plasmid</u> | ||
| + | !<u>Date of plating</u> | ||
| + | !<u>Antibiotics</u><BR> | ||
| + | |- | ||
| + | |<center>0</center> | ||
| + | |Negative control (50 μL) | ||
| + | |1 μL MilliQ | ||
| + | |29august2009 | ||
| + | |LB-Amp<sub>100</sub> | ||
| + | |- | ||
| + | |<center>0</center> | ||
| + | |Negative control (200 μL) | ||
| + | |1 μL MilliQ | ||
| + | |29august2009 | ||
| + | |LB-Amp<sub>100</sub> | ||
| + | |- | ||
| + | |<center>0</center> | ||
| + | |Positive control (50 μL) | ||
| + | |1 μL {{todo|pSB1AC3}}+high constitutive promotor | ||
| + | |29august2009 | ||
| + | |LB-Amp<sub>100</sub> | ||
| + | |- | ||
| + | |<center>0</center> | ||
| + | |Positive control (200 μL) | ||
| + | |1 μL {{todo|pSB1AC3}}+high constitutive promotor | ||
| + | |29august2009 | ||
| + | |LB-Amp<sub>100</sub> | ||
| + | |- | ||
| + | |<center>4</center> | ||
| + | |SmtA+pLac (50 μL) | ||
| + | |pSB1AC3 | ||
| + | |29august2009 | ||
| + | |LB-Amp<sub>100</sub> | ||
| + | |- | ||
| + | |<center>22</center> | ||
| + | |SmtA+pLac (200 μL) | ||
| + | |pSB1AC3 | ||
| + | |29august2009 | ||
| + | |LB-Amp<sub>100</sub> | ||
| + | |- | ||
| + | |<center>37</center> | ||
| + | |SmtA+pLow (50 μL) | ||
| + | |pSB1AC3 | ||
| + | |29august2009 | ||
| + | |LB-Amp<sub>100</sub> | ||
| + | |- | ||
| + | |<center>~72</center> | ||
| + | |SmtA+pLow (200 μL) | ||
| + | |pSB1AC3 | ||
| + | |29august2009 | ||
| + | |LB-Amp<sub>100</sub> | ||
| + | |- | ||
| + | |} | ||
| + | {{todo|pSB1AC3}} was in fact pSB3K3! Therefore no colonies could grow.<BR> 5 colonies of each promotor were inoculated for an ON culture, colony PCR was postponed to monday. | ||
| + | |||
{{Team:Groningen/Notebook/Day/Footer}} | {{Team:Groningen/Notebook/Day/Footer}} | ||
Revision as of 07:50, 30 August 2009
 |
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Weekend stuff
Metal accumulation
Transformation
| Colonies | Name | Plasmid | Date of plating | Antibiotics |
|---|---|---|---|---|
| Negative control (50 μL) | 1 μL MilliQ | 29august2009 | LB-Amp100 | |
| Negative control (200 μL) | 1 μL MilliQ | 29august2009 | LB-Amp100 | |
| Positive control (50 μL) | 1 μL pSB1AC3+high constitutive promotor | 29august2009 | LB-Amp100 | |
| Positive control (200 μL) | 1 μL pSB1AC3+high constitutive promotor | 29august2009 | LB-Amp100 | |
| SmtA+pLac (50 μL) | pSB1AC3 | 29august2009 | LB-Amp100 | |
| SmtA+pLac (200 μL) | pSB1AC3 | 29august2009 | LB-Amp100 | |
| SmtA+pLow (50 μL) | pSB1AC3 | 29august2009 | LB-Amp100 | |
| SmtA+pLow (200 μL) | pSB1AC3 | 29august2009 | LB-Amp100 |
pSB1AC3 was in fact pSB3K3! Therefore no colonies could grow.
5 colonies of each promotor were inoculated for an ON culture, colony PCR was postponed to monday.
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