From 2009.igem.org
Weekend stuff
Metal accumulation
Transformation
SmtA+promotor
Colonies
| Name
| Plasmid
| Date of plating
| Antibiotics
|
0
| Negative control (50 μL)
| 1 μL MilliQ
| 29august2009
| LB-Amp100
|
0
| Negative control (200 μL)
| 1 μL MilliQ
| 29august2009
| LB-Amp100
|
0
| Positive control (50 μL)
| 1 μL pSB1AC3+high constitutive promotor
| 29august2009
| LB-Amp100
|
0
| Positive control (200 μL)
| 1 μL pSB1AC3+high constitutive promotor
| 29august2009
| LB-Amp100
|
4
| SmtA+pLac (50 μL)
| pSB1AC3
| 29august2009
| LB-Amp100
|
22
| SmtA+pLac (200 μL)
| pSB1AC3
| 29august2009
| LB-Amp100
|
37
| SmtA+pLow (50 μL)
| pSB1AC3
| 29august2009
| LB-Amp100
|
~72
| SmtA+pLow (200 μL)
| pSB1AC3
| 29august2009
| LB-Amp100
|
pSB1AC3 was in fact pSB3K3! Therefore no colonies could grow.
5 colonies of each promotor were inoculated for an ON culture, colony PCR was postponed to monday.