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Team:Groningen/Notebook/30 July 2009
From 2009.igem.org
(Difference between revisions)
(New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== ===Transporters=== GlpF Redone PCR3 {| |25uL |Phusion |- |2 uL |GlpF Fw |- |2 uL |GlpF Rev |- |0.5 uL | GlpF PCR3 (27/...) |
(→Transporters) |
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| Line 21: | Line 21: | ||
|- | |- | ||
|0.5 uL | |0.5 uL | ||
| - | | GlpF PCR3 ( | + | | GlpF PCR3 (28/07/09) DNA |
|- | |- | ||
|21uL | |21uL | ||
|MQ | |MQ | ||
| + | |- | ||
| + | |} | ||
| + | |||
| + | The resulting PCR product was purified using a PCR purification kit of Roche. | ||
| + | concentration was measured using Nanodrop; | ||
| + | {| | ||
| + | |16,0 ng/ul | ||
| + | |GlpFPCR3 30/07 | ||
| + | |- | ||
| + | |36.4 ng/ul | ||
| + | |psB1AC3-M digested | ||
| + | |- | ||
| + | |32.0 ng/uL | ||
| + | |GlpF digested | ||
|- | |- | ||
|} | |} | ||
Revision as of 14:06, 30 July 2009
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Wet
GVP Cluster
Transporters
GlpF
Redone PCR3
| 25uL | Phusion |
| 2 uL | GlpF Fw |
| 2 uL | GlpF Rev |
| 0.5 uL | GlpF PCR3 (28/07/09) DNA |
| 21uL | MQ |
The resulting PCR product was purified using a PCR purification kit of Roche. concentration was measured using Nanodrop;
| 16,0 ng/ul | GlpFPCR3 30/07 |
| 36.4 ng/ul | psB1AC3-M digested |
| 32.0 ng/uL | GlpF digested |
Metal Accumulation
Vectors
Dry
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