Team:Groningen/Notebook/30 July 2009

From 2009.igem.org

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===Vectors===
===Vectors===
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[http://openwetware.org/wiki/User:Wilfred_J._Poppinga/Notebook/Wilfreds_Project/2009/07/30 Results] were disappointing, no colonies on plate. Hope the fresh ligase and ligase buffer (10x) will help the new ligation that was started.
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[http://openwetware.org/wiki/User:Wilfred_J._Poppinga/Notebook/Wilfreds_Project/2009/07/30 Results] were disappointing, no colonies on plate. Hope the fresh ligase and ligase buffer (10x) will help the new ligation that was started. New vectors were cut and purified from gel. <BR><center>[[Image:PSB1AC3_%26_pSB3K3_cut_with_SpeI_%26_EcoRI.jpg|500xpx]]</center>
==Dry==
==Dry==

Revision as of 19:58, 30 July 2009

Igemhomelogo.png

Wet

GVP Cluster

Transporters

GlpF

Redone PCR3

25uL Phusion
2 uL GlpF Fw
2 uL GlpF Rev
0.5 uL GlpF PCR3 (28/07/09) DNA
21uL MQ

The resulting PCR product was purified using a PCR purification kit of Roche. concentration was measured using Nanodrop;

16,0 ng/ul GlpFPCR3 30/07
36.4 ng/ul psB1AC3-M digested
32.0 ng/uL GlpF digested

Restiction was done on the GlpF PCR3 (30/07) with XbaI and PstI as noted on the notebook of 28/07/09 The resticted GlpF PCR3 (30/07) was ligated with psB1AC3 resticted with EcoRI and SpeI.

Ligation mix:

2.0 uL T4 Ligase buffer
0.5 uL T4 Ligase
11.25 uL GlpF PCR3

Metal Accumulation

Vectors

Results were disappointing, no colonies on plate. Hope the fresh ligase and ligase buffer (10x) will help the new ligation that was started. New vectors were cut and purified from gel.
PSB1AC3 & pSB3K3 cut with SpeI & EcoRI.jpg

Dry

We worked on migrating graphs from Google spreadsheets to Wiki tables, making sure that at least the raw data used to generate the graphs is "safe".


April
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May
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June
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29 30
July
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August
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31
September
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October
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November
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