Team:Groningen/Notebook/31 July 2009
From 2009.igem.org
(Difference between revisions)
(New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== ===Transporters=== ===Metal Accumulation=== '''ArsR-MBP fusion''' ::colony PCR MBP ::: ::Overnight culture MBP ===V...) |
(→Metal Accumulation) |
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'''ArsR-MBP fusion''' | '''ArsR-MBP fusion''' | ||
- | ::colony PCR MBP | + | ::*'''colony PCR MBP:''' |
- | + | {| | |
- | + | | | |
+ | {| border="1" | ||
+ | |+ '''colony PCR MBP''' | ||
+ | ! Component !! amount | ||
+ | |- | ||
+ | ! 2x Phusion MM | ||
+ | | 12.5 uL | ||
+ | |- | ||
+ | ! MQ | ||
+ | | 9.5 uL | ||
+ | |- | ||
+ | ! MBP Fw | ||
+ | | 1 uL | ||
+ | |- | ||
+ | ! MBP Rev | ||
+ | | 1 uL | ||
+ | |- | ||
+ | ! Colony MBP | ||
+ | | 1 uL | ||
+ | |} | ||
+ | |width="15"| | ||
+ | | | ||
+ | <!--Tabel 2 hier--> | ||
+ | {| | ||
+ | ! Touchdown MBP program | ||
+ | ! Temperature | ||
+ | ! Time | ||
+ | |- | ||
+ | |Denaturing | ||
+ | |98° | ||
+ | |2.00 min | ||
+ | |- | ||
+ | | | ||
+ | |Touchdown 10X 60->50 | ||
+ | |- | ||
+ | |Denaturing | ||
+ | |98° | ||
+ | |30 sec | ||
+ | |- | ||
+ | |Annealing | ||
+ | |60°->50° | ||
+ | |30 sec | ||
+ | |- | ||
+ | |Elongation | ||
+ | |72° | ||
+ | |30 sec | ||
+ | |- | ||
+ | | | ||
+ | |End cycles | ||
+ | |-| | ||
+ | |Start Cycles 25X | ||
+ | |- | ||
+ | |Denaturing | ||
+ | |98° | ||
+ | |30sec | ||
+ | |- | ||
+ | |Annealing | ||
+ | |60° | ||
+ | |30 sec | ||
+ | |- | ||
+ | |Elongation | ||
+ | |72° | ||
+ | |30 sec | ||
+ | |- | ||
+ | | | ||
+ | |End cycles | ||
+ | |- | ||
+ | |Final elongation | ||
+ | |72° | ||
+ | |7 min | ||
+ | |- | ||
+ | |Hold | ||
+ | |4° | ||
+ | |Forever | ||
+ | |} | ||
+ | |width="10"| | ||
+ | |} | ||
+ | ::*'''Overnight culture MBP''' | ||
===Vectors=== | ===Vectors=== |
Revision as of 09:54, 31 July 2009
Wet
GVP Cluster
Transporters
Metal Accumulation
ArsR-MBP fusion
- colony PCR MBP:
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- Overnight culture MBP
Vectors
Dry
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