Team:Groningen/Notebook/31 July 2009

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(Difference between revisions)
(New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== ===Transporters=== ===Metal Accumulation=== '''ArsR-MBP fusion''' ::colony PCR MBP ::: ::Overnight culture MBP ===V...)
(Metal Accumulation)
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'''ArsR-MBP fusion'''
'''ArsR-MBP fusion'''
-
::colony PCR MBP
+
::*'''colony PCR MBP:'''
-
:::  
+
{|
-
::Overnight culture MBP
+
|
 +
{| border="1"
 +
|+ '''colony PCR MBP'''
 +
! Component !! amount
 +
|-
 +
! 2x Phusion MM
 +
| 12.5 uL
 +
|-
 +
! MQ
 +
| 9.5 uL
 +
|-
 +
! MBP Fw
 +
| 1 uL
 +
|-
 +
! MBP Rev
 +
| 1 uL
 +
|-
 +
! Colony MBP
 +
| 1 uL
 +
|}
 +
|width="15"|
 +
|
 +
<!--Tabel 2 hier-->
 +
{|
 +
! Touchdown MBP program
 +
! Temperature
 +
! Time
 +
|-
 +
|Denaturing
 +
|98°
 +
|2.00 min
 +
|-
 +
|
 +
|Touchdown 10X 60->50
 +
|-
 +
|Denaturing
 +
|98°
 +
|30 sec
 +
|-
 +
|Annealing
 +
|60°->50°
 +
|30 sec
 +
|-
 +
|Elongation
 +
|72°
 +
|30 sec
 +
|-
 +
|
 +
|End cycles
 +
|-|
 +
|Start Cycles 25X
 +
|-
 +
|Denaturing
 +
|98°
 +
|30sec
 +
|-
 +
|Annealing
 +
|60°
 +
|30 sec
 +
|-
 +
|Elongation
 +
|72°
 +
|30 sec
 +
|-
 +
|
 +
|End cycles
 +
|-
 +
|Final elongation
 +
|72°
 +
|7 min
 +
|-
 +
|Hold
 +
|4°
 +
|Forever
 +
|}
 +
|width="10"|
 +
|}
 +
::*'''Overnight culture MBP'''
===Vectors===
===Vectors===

Revision as of 09:54, 31 July 2009

Igemhomelogo.png

Wet

GVP Cluster

Transporters

Metal Accumulation

ArsR-MBP fusion

  • colony PCR MBP:
colony PCR MBP
Component amount
2x Phusion MM 12.5 uL
MQ 9.5 uL
MBP Fw 1 uL
MBP Rev 1 uL
Colony MBP 1 uL
Touchdown MBP program Temperature Time
Denaturing 98° 2.00 min
Touchdown 10X 60->50
Denaturing 98° 30 sec
Annealing 60°->50° 30 sec
Elongation 72° 30 sec
End cycles
Start Cycles 25X
Denaturing 98° 30sec
Annealing 60° 30 sec
Elongation 72° 30 sec
End cycles
Final elongation 72° 7 min
Hold Forever
  • Overnight culture MBP

Vectors

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30