Team:Groningen/Notebook/31 July 2009
From 2009.igem.org
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===Transporters=== | ===Transporters=== | ||
+ | '''GlpF''' | ||
+ | *Restiction/ligase and transformation of GlpF in psB1AC3 | ||
+ | Restiction | ||
+ | {| | ||
+ | |10ul | ||
+ | |GlpF PCR | ||
+ | |- | ||
+ | |2 ul | ||
+ | |Buffer | ||
+ | |- | ||
+ | |05.ul | ||
+ | |EcoRI | ||
+ | |- | ||
+ | |0.5 ul | ||
+ | |SpeI | ||
+ | |- | ||
+ | |7 uL | ||
+ | |MQ | ||
+ | |- | ||
+ | |} | ||
===Metal Accumulation=== | ===Metal Accumulation=== |
Revision as of 10:00, 31 July 2009
Wet
GVP Cluster
Transporters
GlpF
- Restiction/ligase and transformation of GlpF in psB1AC3
Restiction
10ul | GlpF PCR |
2 ul | Buffer |
05.ul | EcoRI |
0.5 ul | SpeI |
7 uL | MQ |
Metal Accumulation
ArsR-MBP fusion
- colony PCR MBP:
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- Overnight culture MBP
Vectors
Dry
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