Team:Groningen/Notebook/5 September 2009

From 2009.igem.org

(Difference between revisions)
m (New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== ===Transporters=== ===Metal Accumulation=== Plasmid isolation of [[Team:Groningen/Notebook/24_August_2009#Metal_Accumul...)
(GVP Cluster)
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===GVP Cluster===
===GVP Cluster===
 +
{| border="1"
 +
|+'''Michaels plates'''
 +
!<u><i>E. coli</i> TOP10 Colonies</u>
 +
!<u>Name</u>
 +
!<u>Plasmid</u>
 +
!<u>Date of plating</u>
 +
!<u>Antibiotics</u><BR>
 +
|-
 +
|<center>92</center>
 +
|pCueO-GVP conc.
 +
|<partinfo>pSB2K3</partinfo>
 +
|4september2009
 +
|Kanamycin/IPTG
 +
|-
 +
|<center>38</center>
 +
|pZntR-GVP conc.
 +
|<partinfo>pSB2K3</partinfo>
 +
|4september2009
 +
|Kanamycin/IPTG
 +
|-
 +
|<center>35</center>
 +
|pArsR-GVP conc.
 +
|<partinfo>pSB2K3</partinfo>
 +
|4september2009
 +
|Kanamycin/IPTG
 +
|-
 +
|<center>41</center>
 +
|pLac-GVP conc.
 +
|<partinfo>pSB2K3</partinfo>
 +
|4september2009
 +
|Kanamycin/IPTG
 +
|-
 +
|<center>0</center>
 +
|(7K)
 +
|<partinfo>pSB2K3</partinfo>
 +
|4september2009
 +
|Kanamycin/IPTG
 +
|-
 +
|<center>0</center>
 +
|(7K) conc.
 +
|<partinfo>pSB2K3</partinfo>
 +
|4september2009
 +
|Kanamycin/IPTG
 +
|-
 +
|<center>{{todo|1}}</center>
 +
|(7C)
 +
|<partinfo>pSB2K3</partinfo>
 +
|4september2009
 +
|Kanamycin/IPTG
 +
|-
 +
|<center>1</center>
 +
|(7C) conc.
 +
|<partinfo>pSB2K3</partinfo>
 +
|4september2009
 +
|Kanamycin/IPTG
 +
|-
 +
|}
 +
Plates with colonies were stored in fridge, the {{todo|red}} indicates a red colony. Plates without colonies were left on the bench.
===Transporters===
===Transporters===

Revision as of 10:27, 5 September 2009

Igemhomelogo.png

Wet

GVP Cluster

Michaels plates
E. coli TOP10 Colonies Name Plasmid Date of plating Antibiotics
92
pCueO-GVP conc. pSB2K3 4september2009 Kanamycin/IPTG
38
pZntR-GVP conc. pSB2K3 4september2009 Kanamycin/IPTG
35
pArsR-GVP conc. pSB2K3 4september2009 Kanamycin/IPTG
41
pLac-GVP conc. pSB2K3 4september2009 Kanamycin/IPTG
0
(7K) pSB2K3 4september2009 Kanamycin/IPTG
0
(7K) conc. pSB2K3 4september2009 Kanamycin/IPTG
1
(7C) pSB2K3 4september2009 Kanamycin/IPTG
1
(7C) conc. pSB2K3 4september2009 Kanamycin/IPTG

Plates with colonies were stored in fridge, the red indicates a red colony. Plates without colonies were left on the bench.

Transporters

Metal Accumulation

Plasmid isolation of SmtA-1 ON culture

Vectors

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30