Team:Groningen/Notebook/8 August 2009

From 2009.igem.org

Revision as of 13:25, 8 August 2009 by Wilfred (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Igemhomelogo.png

Wet

Miscellaneous

Frans' plates
Colonies Name Plasmid Date of plating Antibiotics
0
K3 pBad 2:3 pSB3K3 7august2009 50 μg/mL kan
0
K3 pBad 2:3 pSB3K3 7august2009 50 μg/mL kan
0
K3 pBad 2:4 pSB3K3 7august2009 50 μg/mL kan
3
K3 pBad 2:4 pSB3K3 7august2009 50 μg/mL kan
~150
AC3 p4od 1:3 pSB1AC3 7august2009 LB AMP
~30
AC3 pBad 1:3 pSB1AC3 7august2009 LB AMP
1
AC3 Lac 1:4 pSB1AC3 7august2009 LB AMP
3
AC3 Lac 1:4 pSB1AC3 7august2009 LB AMP

After 5 h at 37 °C (and ON on the bench) Codes are written as they are read, not as they are interpreted. Plates with no growth were put back on the bench.

GVP Cluster

Transporters

Metal Accumulation

Nienke's plates MymT
Colonies Name Plasmid Date of plating Antibiotics
226
MymT Conc pSB68(MymT) 7august2009 LB-Amp
∞/10
pSB1AC3-lowP 7august2009 LB-Cam150
12
MymT p4B68(MymT) 7august2009 LB-Amp
0
–-
pSB1AC3 7august2009 LB-Cam150
(+)
pSB1AC3-lowProm 7august2009 LB-Cam150
>400
(MymT Conc) pGB68(MymT) conc. 7august2009 LB-Amp100
>400
(MymT) pGB68(MymT) 7august2009 LB-Amp
4
–-
pSB1AC3 7august2009 LB-Cam150

Everything was written as I read it, no guarantees.


Vectors

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30