Team:Groningen/Parts

From 2009.igem.org

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(Part List -80)
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{{Team:Groningen/Header}}
{{Team:Groningen/Header}}
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<!--- The Mission, Experiments --->
 
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===Note===
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<div style="float:left" >{{linkedImage|GroningenPrevious.png|Team:Groningen/Ethics/Survey}}</div>
 +
<div title="Arsie Says UP TO ACCUMULATION" style="float:right">{{linkedImage|Next.JPG|Team:Groningen/Parts/Submitted_Parts}}</div>
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If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here.  This is not necessary but it may be a nice list to keep track of.
 
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:::::::::::::::http://partsregistry.org/wiki/images/d/db/Buttonaddpart.gif
 
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<b><h2>[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen Our sandbox]</h2></b>
 
 +
*For a comprehensive list of all the parts we used, have a look at our [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen '''parts registry'''].
 +
*Here you can find an informative overview of our [[Team:Groningen/Modelling/Submitted Parts|'''submitted parts''']].
 +
*Experience we had with parts from the registry can be found under [[Team:Groningen/Modelling/Used Parts|'''used parts''']].
 +
*Need anything specific? Take a look in our [[Team:Groningen/Parts/Freezer|freezer]]
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===Part List -80===
 
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{|cellpadding="10" cellspacing="1" border="10" style="font-size: large;"
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|'''Component'''
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{{Team:Groningen/Footer}}
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|'''Description'''
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|'''Part or Accession #'''
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|'''Base Pairs (bp)'''
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|'''Plasmid (backbone)'''
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|'''Resistance'''
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|'''Well'''
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|'''Quality control'''
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|-
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|GVP
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|Gas Vesicle Proteins
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|[http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
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|6064
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|[http://partsregistry.org/Part:BBa_J61035 BBa_J61035]
+
-
|Ampicillin/Gentamycin
+
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|plate 3, pos.1
+
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|?
+
-
|-
+
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|GVP (2nd batch)
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|Gas Vesicle Proteins
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|[http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
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|6064
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|[http://partsregistry.org/Part:BBa_J61035 BBa_J61035]
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|Ampicillin/Gentamycin
+
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|plate 3, pos.2
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|?
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|-
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|pHigh in low copy nr vector
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|High consitutive promoter
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|<partinfo>J23100</partinfo>
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|35bp
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|<partinfo>pSB3K3</partinfo>
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|Kanamycin
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|Plate 3, pos. 4
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|PCR(?), restriction(+)
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|-
+
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|pMed in low copy nr vector
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|Medium consitutive promoter
+
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|<partinfo>J23106</partinfo>
+
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|35bp
+
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|<partinfo>pSB3K3</partinfo>
+
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|Kanamycin
+
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|Plate 3, pos. 5
+
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|PCR(?), restriction(+)
+
-
|-
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|pLow in low copy nr vector
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|Low consitutive promoter
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|<partinfo>J23109</partinfo>
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|35bp
+
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|<partinfo>pSB3K3</partinfo>
+
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|Kanamycin
+
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|Plate 3, pos. 6
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|PCR(?), restriction(+)
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|-
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|pHigh in high copy nr vector
+
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|High consitutive promoter
+
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|<partinfo>J23100</partinfo>
+
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|35bp
+
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|<partinfo>pSB1AC3</partinfo>
+
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|Ampicillin/Chloramphenicol
+
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|Plate 3, pos. 7
+
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|PCR(?), restriction(+)
+
-
|-
+
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|pMed in high copy nr vector
+
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|Medium consitutive promoter
+
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|<partinfo>J23106</partinfo>
+
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|35bp
+
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|<partinfo>pSB1AC3</partinfo>
+
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|Ampicillin/Chloramphenicol
+
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|Plate 3, pos. 8
+
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|PCR(?), restriction(+)
+
-
|-
+
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|pLow in high copy nr vector
+
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|High consitutive promoter
+
-
|<partinfo>J23109</partinfo>
+
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|35bp
+
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|<partinfo>pSB1AC3</partinfo>
+
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|Ampicillin/Chloramphenicol
+
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|Plate 3, pos. 9
+
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|PCR(?), restriction(+)
+
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|-
+
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|Promotor (low expression)
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|Constitutive promotor
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|[http://partsregistry.org/Part:BBa_J23109 BBa_J23109]
+
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|35
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|[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
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|Ampicillin
+
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|plate 3, pos.10
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|No
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|-
+
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|Promotor (medium expression)
+
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|Constitutive promotor
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|[http://partsregistry.org/Part:BBa_J23106 BBa_J23106]
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|35
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|[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
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|Ampicillin
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|plate 3, pos.11
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|No
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|-
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|Promotor (high expression)
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|Constitutive promotor
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|[http://partsregistry.org/Part:BBa_J23100 BBa_J23100]
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|35
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|[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
+
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|Ampicillin
+
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|plate 3, pos.12
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|No
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|-
+
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|Inducible promoter
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|Lactose inducible promoter nr1
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|<partinfo>R0010</partinfo>
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|200bp
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|<partinfo>pSB1A2</partinfo>
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|Ampicillin
+
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|Plate 3, pos. 14
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|PCR(?), restriction(+)
+
-
|-
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|Inducible promoter
+
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|Lactose inducible promoter nr2
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|<partinfo>R0010</partinfo>
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|200bp
+
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|<partinfo>pSB1A2</partinfo>
+
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|Ampicillin
+
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|Plate 3, pos. 15
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|PCR(?), restriction(+)
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|-
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|RBS
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|Ribosomal Binding Site
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|[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]
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|12
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|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
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|Ampicillin
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|plate 3, pos.19
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|No
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|-
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|Terminator
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|Double terminator consisting of [http://partsregistry.org/Part:BBa_B0012 BBa_B0012] and [http://partsregistry.org/Part:BBa_B0011 BBa_B0011]
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|[http://partsregistry.org/Part:BBa_B0014 BBa_B0014]
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|~90
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|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
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|Ampicillin/Kanamycin
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|plate 3, pos.20
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|No
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|-
+
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|Const. Pormoter + GVP
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|Low constitutive promoter with GVP cluster
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|[http://partsregistry.org/Part:BBa_J23109 BBa_J23109] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
+
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|~6100
+
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|[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
+
-
|Ampicillin/Chloramphenicol
+
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|plate 3, pos.21
+
-
|EcoRI/PstI
+
-
|-
+
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|Const. Pormoter + GVP (no.2)
+
-
|Low constitutive promoter with GVP cluster
+
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|[http://partsregistry.org/Part:BBa_J23109 BBa_J23109] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
+
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|~6100
+
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|[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
+
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|Ampicillin/Chloramphenicol
+
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|plate 3, pos.22
+
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|EcoRI/PstI
+
-
|-
+
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|Const. Pormoter + GVP
+
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|Medium constitutive promoter with GVP cluster
+
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|[http://partsregistry.org/Part:BBa_J23106 BBa_J23106] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
+
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|~6100
+
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|[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
+
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|Ampicillin/Chloramphenicol
+
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|plate 3, pos.23
+
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|EcoRI/PstI
+
-
|-
+
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|Const. Pormoter + GVP (no.2)
+
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|Medium constitutive promoter with GVP cluster
+
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|[http://partsregistry.org/Part:BBa_J23106 BBa_J23106] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
+
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|~6100
+
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|[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
+
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|Ampicillin/Chloramphenicol
+
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|plate 3, pos.24
+
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|EcoRI/PstI
+
-
|-
+
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|HmtA (1.1)
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|HmtA-His in pBAD(a)
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|None
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|?
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|None
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|Ampicillin
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|plate 3, pos.28
+
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|Restriction(+)
+
-
|-
+
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|HmtA (2.1)
+
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|HmtA-His in pBAD(a)
+
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|None
+
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|?
+
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|None
+
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|Ampicillin
+
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|plate 3, pos.29
+
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|Restriction(+)
+
-
|-
+
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|CueO- + GVP in J61035
+
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|CueO promoter without RBS in front of the GVP cluster
+
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|None
+
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|6149
+
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI)
+
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|Ampicillin
+
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|plate 3, pos.37
+
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|Restriction(+)
+
-
|-
+
-
|ZntR- + GVP in J61035
+
-
|ZntR promoter without RBS in front of the GVP cluster
+
-
|None
+
-
|~6150
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI)
+
-
|Ampicillin
+
-
|plate 3, pos.38
+
-
|Restriction(+)
+
-
|-
+
-
|ArsR- + GVP in J61035
+
-
|ArsR promoter without RBS in front of the GVP cluster
+
-
|None
+
-
|~6150
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI)
+
-
|Ampicillin
+
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|plate 3, pos.39
+
-
|Restriction(+)
+
-
|-
+
-
|ArsR- in J61002
+
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|pArsR (promoter without RBS)
+
-
|BBa_K190015
+
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|71
+
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
+
-
|Ampicillin
+
-
|plate 3, pos.40
+
-
|EcoRI/SpeI(+), EcoRI/PstI(+)
+
-
|-
+
-
|ZntR- in J61002
+
-
|pZntR (promoter without RBS)
+
-
|BBa_K190016
+
-
|65
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
+
-
|Ampicillin
+
-
|plate 3, pos.41
+
-
|EcoRI/SpeI(+), EcoRI/PstI(+)
+
-
|-
+
-
|CueO- in J61002
+
-
|pCueO (promoter without RBS)
+
-
|BBa_K190017
+
-
|43
+
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
+
-
|Ampicillin
+
-
|plate 3, pos.42
+
-
|EcoRI/SpeI(+), EcoRI/PstI(+)
+
-
|-
+
-
|SmtA-GST in pGEX-3x
+
-
|SmtA-GST fusion
+
-
|no biobrick primers available
+
-
|1400bp
+
-
|pGEX-3x (NEB)
+
-
|Ampicillin
+
-
|plate 3, pos. 43
+
-
|PCR, restriction
+
-
|-
+
-
|SmtA in pET29a
+
-
|SmtA
+
-
|<partinfo>BBa_K190021</partinfo>
+
-
|900bp
+
-
|pET29a (NEB)
+
-
|Kanamycin
+
-
|plate 3, pos. 44
+
-
|PCR, restriction
+
-
|-
+
-
|MymT in pGB68
+
-
|MymT
+
-
|<partinfo>BBa_K190020</partinfo>
+
-
|215bp
+
-
|Ampicillin
+
-
|pTXB1 (NEB)
+
-
|plate 3, pos. 45
+
-
|PCR, restriction
+
-
|-
+
-
|GlpF and fMT in pMAL-MT
+
-
|GlpF and fMT on a synthetic operon in the vector
+
-
|<partinfo>BBa_K190019</partinfo> and BBa_K1900?? (GlpF has no BioBrick nr yet)
+
-
|fMT = 200bp, GlpF = 900bp?
+
-
|Ampicillin
+
-
|pMALc2x (NEB)
+
-
|plate 3, pos. 46
+
-
|PCR, restriction
+
-
|-
+
-
|MBP-ArsR
+
-
|MBP-ArsR Fusion protein
+
-
|{{todo|}}
+
-
|1500 bp
+
-
|pSB1AC3
+
-
|Ampicillin/chloramphenicol
+
-
|plate 3, pos. 53
+
-
|[https://2009.igem.org/Team:Groningen/Notebook/18_August_2009#Metal_accumulation| Restriction analysis] & [[Team:Groningen/Notebook/25_August_2009#Metal_Accumulation| PCR]]
+
-
|-
+
-
|pNL29
+
-
|GVP Gene cluster
+
-
|{{todo|}}
+
-
|6000 bp
+
-
|pBluescriptIIKS
+
-
|{{todo| Ampicillin}}
+
-
|plate 3, pos. 54
+
-
|{{todo|Buoyancy phenotype}}
+
-
|-
+
-
|pLac-fMT
+
-
|Metallothionein with inducible promotor
+
-
|{{todo|Part or Accession #}}
+
-
|{{todo|Base Pairs (bp)}}
+
-
|pSB1AC3
+
-
|Ampicillin/chloramphenicol
+
-
|{{todo|Well}}
+
-
|{{todo|Quality control}}
+
-
|-
+
-
|pLow-fMT #4
+
-
|Metallothionein with low constitutive promotor
+
-
|{{todo|Part or Accession #}}
+
-
|{{todo|Base Pairs (bp)}}
+
-
|pSB1AC3
+
-
|Ampicillin/chloramphenicol
+
-
|{{todo|Well}}
+
-
|{{todo|Quality control}}
+
-
|-
+
-
|pLow-fMT #3
+
-
|Metallothionein with low constitutive promotor
+
-
|{{todo|Part or Accession #}}
+
-
|{{todo|Base Pairs (bp)}}
+
-
|pSB1AC3
+
-
|Ampicillin/chloramphenicol
+
-
|{{todo|Well}}
+
-
|{{todo|Quality control}}
+
-
|-
+
-
|SmtA
+
-
|Metallothionein
+
-
|{{todo|Part or Accession #}}
+
-
|{{todo|Base Pairs (bp)}}
+
-
|pSB1AC3
+
-
|Ampicillin/chloramphenicol
+
-
|{{todo|Well}}
+
-
|{{todo|Quality control}}
+
-
|-
+
-
|fMT #6
+
-
|Metallothionein
+
-
|{{todo|Part or Accession #}}
+
-
|{{todo|Base Pairs (bp)}}
+
-
|pSB1AC3
+
-
|Ampicillin/chloramphenicol
+
-
|{{todo|Well}}
+
-
|{{todo|Quality control}}
+
-
|}
+

Latest revision as of 09:41, 22 January 2010

Igemhomelogo.png
Biobricks


GroningenPrevious.png
Next.JPG



  • For a comprehensive list of all the parts we used, have a look at our parts registry.
  • Here you can find an informative overview of our submitted parts.
  • Experience we had with parts from the registry can be found under used parts.
  • Need anything specific? Take a look in our freezer