Team:Groningen/Parts

From 2009.igem.org

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{{Team:Groningen/Header}}
{{Team:Groningen/Header}}
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<!--- The Mission, Experiments --->
 
-
===Note===
+
<div style="float:left" >{{linkedImage|GroningenPrevious.png|Team:Groningen/Ethics/Survey}}</div>
 +
<div title="Arsie Says UP TO ACCUMULATION" style="float:right">{{linkedImage|Next.JPG|Team:Groningen/Parts/Submitted_Parts}}</div>
-
If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here.  This is not necessary but it may be a nice list to keep track of.
 
-
:::::::::::::::http://partsregistry.org/wiki/images/d/db/Buttonaddpart.gif
 
-
<b><h2>[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen Our sandbox]</h2></b>
 
 +
*For a comprehensive list of all the parts we used, have a look at our [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen '''parts registry'''].
 +
*Here you can find an informative overview of our [[Team:Groningen/Modelling/Submitted Parts|'''submitted parts''']].
 +
*Experience we had with parts from the registry can be found under [[Team:Groningen/Modelling/Used Parts|'''used parts''']].
 +
*Need anything specific? Take a look in our [[Team:Groningen/Parts/Freezer|freezer]]
-
===Part List -80===
 
-
{|cellpadding="10" cellspacing="1" border="10" style="font-size: large;"
+
 
-
|'''Component'''
+
{{Team:Groningen/Footer}}
-
|'''Description'''
+
-
|'''Part or Accession #'''
+
-
|'''Base Pairs (bp)'''
+
-
|'''Plasmid (backbone)'''
+
-
|'''Resistance'''
+
-
|'''Well'''
+
-
|'''Quality control'''
+
-
|-
+
-
|GVP
+
-
|Gas Vesicle Proteins
+
-
|<partinfo>BBa_I750016</partinfo>
+
-
|6064
+
-
|<partinfo>BBa_J61035</partinfo>
+
-
|Ampicillin/Gentamycin
+
-
|plate 3, pos.1
+
-
|?
+
-
|-
+
-
|GVP (2nd batch)
+
-
|Gas Vesicle Proteins
+
-
|<partinfo>BBa_I750016</partinfo>
+
-
|6064
+
-
|<partinfo>BBa_J61035</partinfo>
+
-
|Ampicillin/Gentamycin
+
-
|plate 3, pos.2
+
-
|?
+
-
|-
+
-
|pHigh in low copy nr vector
+
-
|High consitutive promoter
+
-
|<partinfo>J23100</partinfo>
+
-
|35
+
-
|<partinfo>pSB3K3</partinfo>
+
-
|Kanamycin
+
-
|Plate 3, pos. 4
+
-
|PCR(?), restriction(+)
+
-
|-
+
-
|pMed in low copy nr vector
+
-
|Medium consitutive promoter
+
-
|<partinfo>J23106</partinfo>
+
-
|35
+
-
|<partinfo>pSB3K3</partinfo>
+
-
|Kanamycin
+
-
|Plate 3, pos. 5
+
-
|PCR(?), restriction(+)
+
-
|-
+
-
|pLow in low copy nr vector
+
-
|Low consitutive promoter
+
-
|<partinfo>J23109</partinfo>
+
-
|35
+
-
|<partinfo>pSB3K3</partinfo>
+
-
|Kanamycin
+
-
|Plate 3, pos. 6
+
-
|PCR(?), restriction(+)
+
-
|-
+
-
|pHigh in high copy nr vector
+
-
|High consitutive promoter
+
-
|<partinfo>J23100</partinfo>
+
-
|35
+
-
|<partinfo>pSB1AC3</partinfo>
+
-
|Ampicillin/Chloramphenicol
+
-
|Plate 3, pos. 7
+
-
|PCR(?), restriction(+)
+
-
|-
+
-
|pMed in high copy nr vector
+
-
|Medium consitutive promoter
+
-
|<partinfo>J23106</partinfo>
+
-
|35
+
-
|<partinfo>pSB1AC3</partinfo>
+
-
|Ampicillin/Chloramphenicol
+
-
|Plate 3, pos. 8
+
-
|PCR(?), restriction(+)
+
-
|-
+
-
|pLow in high copy nr vector
+
-
|High consitutive promoter
+
-
|<partinfo>J23109</partinfo>
+
-
|35
+
-
|<partinfo>pSB1AC3</partinfo>
+
-
|Ampicillin/Chloramphenicol
+
-
|Plate 3, pos. 9
+
-
|PCR(?), restriction(+)
+
-
|-
+
-
|Promotor (low expression)
+
-
|Constitutive promotor
+
-
|<partinfo>BBa_J23109</partinfo>
+
-
|35
+
-
|<partinfo>BBa_J61002</partinfo>
+
-
|Ampicillin
+
-
|plate 3, pos.10
+
-
|No
+
-
|-
+
-
|Promotor (medium expression)
+
-
|Constitutive promotor
+
-
|<partinfo>BBa_J23106</partinfo>
+
-
|35
+
-
|<partinfo>BBa_J61002</partinfo>
+
-
|Ampicillin
+
-
|plate 3, pos.11
+
-
|No
+
-
|-
+
-
|Promotor (high expression)
+
-
|Constitutive promotor
+
-
|<partinfo>BBa_J23100</partinfo>
+
-
|35
+
-
|<partinfo>BBa_J61002</partinfo>
+
-
|Ampicillin
+
-
|plate 3, pos.12
+
-
|No
+
-
|-
+
-
|Inducible promoter
+
-
|Lactose inducible promoter nr1
+
-
|<partinfo>R0010</partinfo>
+
-
|200
+
-
|<partinfo>pSB1A2</partinfo>
+
-
|Ampicillin
+
-
|Plate 3, pos. 14
+
-
|PCR(?), restriction(+)
+
-
|-
+
-
|Inducible promoter
+
-
|Lactose inducible promoter nr2
+
-
|<partinfo>R0010</partinfo>
+
-
|200
+
-
|<partinfo>pSB1A2</partinfo>
+
-
|Ampicillin
+
-
|Plate 3, pos. 15
+
-
|PCR(?), restriction(+)
+
-
|-
+
-
|RBS
+
-
|Ribosomal Binding Site
+
-
|<partinfo>BBa_B0034</partinfo>
+
-
|12
+
-
|<partinfo>pSB1A2</partinfo>
+
-
|Ampicillin
+
-
|plate 3, pos.19
+
-
|No
+
-
|-
+
-
|Terminator
+
-
|Double terminator consisting of <partinfo>BBa_B0012</partinfo> and <partinfo>BBa_B0011</partinfo>
+
-
|<partinfo>BBa_B0014</partinfo>
+
-
|~90
+
-
|<partinfo>pSB1AK3</partinfo>
+
-
|Ampicillin/Kanamycin
+
-
|plate 3, pos.20
+
-
|No
+
-
|-
+
-
|Const. Pormoter + GVP
+
-
|Low constitutive promoter with GVP cluster
+
-
|<partinfo>BBa_J23109</partinfo> + <partinfo>BBa_I750016</partinfo>
+
-
|~6100
+
-
|<partinfo>pSB1AC3</partinfo>
+
-
|Ampicillin/Chloramphenicol
+
-
|plate 3, pos.21
+
-
|<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}}
+
-
|-
+
-
|Const. Pormoter + GVP (no.2)
+
-
|Low constitutive promoter with GVP cluster
+
-
|<partinfo>BBa_J23109</partinfo> + <partinfo>BBa_I750016</partinfo>
+
-
|~6100
+
-
|<partinfo>pSB1AC3</partinfo>
+
-
|Ampicillin/Chloramphenicol
+
-
|plate 3, pos.22
+
-
|<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}}
+
-
|-
+
-
|Const. Pormoter + GVP
+
-
|Medium constitutive promoter with GVP cluster
+
-
|<partinfo>BBa_J23106</partinfo> + <partinfo>BBa_I750016</partinfo>
+
-
|~6100
+
-
|<partinfo>pSB1AC3</partinfo>
+
-
|Ampicillin/Chloramphenicol
+
-
|plate 3, pos.23
+
-
|<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}}
+
-
|-
+
-
|Const. Pormoter + GVP (no.2)
+
-
|Medium constitutive promoter with GVP cluster
+
-
|<partinfo>BBa_J23106</partinfo> + <partinfo>BBa_I750016</partinfo>
+
-
|~6100
+
-
|<partinfo>pSB1AC3</partinfo>
+
-
|Ampicillin/Chloramphenicol
+
-
|plate 3, pos.24
+
-
|<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}}
+
-
|-
+
-
|HmtA (1.1)
+
-
|HmtA-His in pBAD(a)
+
-
|<partinfo>BBa_K190018</partinfo>
+
-
|2004
+
-
|[http://ecoliwiki.net/colipedia/index.php/pBAD/myc-His_A pBAD]
+
-
|Ampicillin
+
-
|plate 3, pos.28
+
-
|Restriction(+)
+
-
|-
+
-
|HmtA (2.1)
+
-
|HmtA-His in pBAD(a)
+
-
|<partinfo>BBa_K190018</partinfo>
+
-
|2004
+
-
|[http://ecoliwiki.net/colipedia/index.php/pBAD/myc-His_A pBAD]
+
-
|Ampicillin
+
-
|plate 3, pos.29
+
-
|Restriction(+)
+
-
|-
+
-
|CueO- + GVP in J61035
+
-
|CueO promoter without RBS in front of the GVP cluster
+
-
|None
+
-
|6149
+
-
|<partinfo>BBa_J61035</partinfo> (without the part between <i>Eco</i>RI and <i>Xba</i>I)
+
-
|Ampicillin
+
-
|plate 3, pos.37
+
-
|Restriction(+) + {{done|Sequenced Okay}}
+
-
|-
+
-
|ZntR- + GVP in J61035
+
-
|ZntR promoter without RBS in front of the GVP cluster
+
-
|None
+
-
|~6150
+
-
|<partinfo>BBa_J61035</partinfo> (without the part between <i>Eco</i>RI and <i>Xba</i>I)
+
-
|Ampicillin
+
-
|plate 3, pos.38
+
-
|Restriction(+) + {{done|Sequenced (3 mutations)}}
+
-
|-
+
-
|ArsR- + GVP in J61035
+
-
|ArsR promoter without RBS in front of the GVP cluster
+
-
|None
+
-
|~6150
+
-
|<partinfo>BBa_J61035</partinfo> (without the part between <i>Eco</i>RI and <i>Xba</i>I)
+
-
|Ampicillin
+
-
|plate 3, pos.39
+
-
|Restriction(+) + {{done|Sequenced Okay}}
+
-
|-
+
-
|ArsR- in J61002
+
-
|pArsR (promoter without RBS)
+
-
|<partinfo>BBa_K190015</partinfo>
+
-
|71
+
-
|<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
+
-
|Ampicillin
+
-
|plate 3, pos.40
+
-
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
+
-
|-
+
-
|ZntR- in J61002
+
-
|pZntR (promoter without RBS)
+
-
|<partinfo>BBa_K190016</partinfo>
+
-
|65
+
-
|<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
+
-
|Ampicillin
+
-
|plate 3, pos.41
+
-
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced (3 mutations)}}
+
-
|-
+
-
|CueO- in J61002
+
-
|pCueO (promoter without RBS)
+
-
|<partinfo>BBa_K190017</partinfo>
+
-
|43
+
-
|<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
+
-
|Ampicillin
+
-
|plate 3, pos.42
+
-
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
+
-
|-
+
-
|ZntR+RBS in J61002
+
-
|pZntR (promoter with own RBS)
+
-
|<partinfo>BBa_K190022</partinfo>
+
-
|91
+
-
|<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
+
-
|Ampicillin
+
-
|plate 3, pos.43
+
-
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
+
-
|-
+
-
|ArsR+RBS in J61002
+
-
|pArsR (promoter with own RBS)
+
-
|<partinfo>BBa_K190023</partinfo>
+
-
|81
+
-
|<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
+
-
|Ampicillin
+
-
|plate 3, pos.44
+
-
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
+
-
|-
+
-
|CueO+RBS in J61002
+
-
|pCueO (promoter with own RBS)
+
-
|<partinfo>BBa_K190024</partinfo>
+
-
|71
+
-
|<partinfo>BBa_J61002</partinfo> (without the part between EcoRI and SpeI)
+
-
|Ampicillin
+
-
|plate 3, pos.45
+
-
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
+
-
|-
+
-
|SmtA-GST in pGEX-3x
+
-
|SmtA-GST fusion
+
-
|no biobrick primers available
+
-
|1400
+
-
|[http://www.ecoliwiki.net/colipedia/index.php/pGEX-3X pGEX-3x]
+
-
|Ampicillin
+
-
|plate 3, pos. 46
+
-
|PCR, restriction
+
-
|-
+
-
|SmtA in pET29a
+
-
|SmtA
+
-
|<partinfo>BBa_K190021</partinfo>
+
-
|900
+
-
|[http://www.merckbiosciences.co.uk/product/69871 pET29a]
+
-
|Kanamycin
+
-
|plate 3, pos. 47
+
-
|PCR, restriction
+
-
|-
+
-
|MymT in pGB68
+
-
|MymT
+
-
|<partinfo>BBa_K190020</partinfo>
+
-
|215
+
-
|[http://www.neb.com/nebecomm/products/productN6707.asp pTXB1] (NEB)
+
-
|Ampicillin
+
-
|plate 3, pos. 48
+
-
|PCR, restriction
+
-
|-
+
-
|GlpF and fMT in pMAL-MT
+
-
|GlpF and fMT on a synthetic operon in the vector
+
-
|<partinfo>BBa_K190019</partinfo> and <partinfo>BBa_K190028</partinfo>
+
-
|fMT = 200, GlpF = 900?
+
-
|[http://www.neb.com/nebecomm/products/productN8076.asp pMALc2x] (NEB)
+
-
|Ampicillin
+
-
|plate 3, pos. 49
+
-
|PCR, restriction
+
-
|-
+
-
|MBP-ArsR
+
-
|MBP-ArsR Fusion protein
+
-
|<partinfo>BBa_K190027</partinfo>
+
-
|15180
+
-
|<partinfo>pSB1AC3</partinfo>
+
-
|Ampicillin/chloramphenicol
+
-
|plate 3, pos. 53
+
-
|[http://2009.igem.org/Team:Groningen/Notebook/18_August_2009#Metal_accumulation| Restriction analysis] & [[Team:Groningen/Notebook/25_August_2009#Metal_Accumulation| PCR]]
+
-
|-
+
-
|pNL29
+
-
|GVP Gene cluster
+
-
|{{todo|}}
+
-
|6000
+
-
|[http://www.fermentas.com/techinfo/nucleicacids/mappbluescriptiiskks.htm pBluescriptIIKS]
+
-
|{{todo| Ampicillin}}
+
-
|plate 3, pos. 54
+
-
|{{todo|Buoyancy phenotype}}
+
-
|-
+
-
|pLac-fMT
+
-
|Metallothionein with inducible promotor
+
-
|<partinfo>BBa_K190032</partinfo>
+
-
|430
+
-
|<partinfo>pSB1A2</partinfo>
+
-
|Ampicillin/chloramphenicol
+
-
|plate 3, pos. 55
+
-
|{{todo|Quality control}}
+
-
|-
+
-
|pLow-fMT #4
+
-
|Metallothionein with low constitutive promotor
+
-
|<partinfo>BBa_K190031</partinfo>
+
-
|265
+
-
|<partinfo>pSB1AC3</partinfo>
+
-
|Ampicillin/chloramphenicol
+
-
|plate 3, pos. 56
+
-
|{{todo|Quality control}}
+
-
|-
+
-
|pLow-fMT #3
+
-
|Metallothionein with low constitutive promotor
+
-
|<partinfo>BBa_K190031</partinfo>
+
-
|265
+
-
|<partinfo>pSB1AC3</partinfo>
+
-
|Ampicillin/chloramphenicol
+
-
|plate 3, pos. 57
+
-
|{{todo|Quality control}}
+
-
|-
+
-
|SmtA
+
-
|Metallothionein
+
-
|<partinfo>BBa_K190021</partinfo>
+
-
|678
+
-
|<partinfo>pSB1AC3</partinfo>
+
-
|Ampicillin/chloramphenicol
+
-
|plate 3, pos. 58
+
-
|{{todo|Quality control}}
+
-
|-
+
-
|fMT #6
+
-
|Metallothionein for As<sup>3+</sup>
+
-
|<partinfo>BBa_K190019</partinfo>
+
-
|222
+
-
|<partinfo>pSB1AC3</partinfo>
+
-
|Ampicillin/chloramphenicol
+
-
|plate 3, pos. 59
+
-
|{{todo|Sequence 2 deletions between <i>Eco</i>RI and <i>Xba</i>I sites}}
+
-
|-
+
-
|fMT #4
+
-
|Metallothionein for As<sup>3+</sup>
+
-
|<partinfo>BBa_K190019</partinfo>
+
-
| 222
+
-
|<partinfo>pSB1AC3</partinfo>
+
-
|Ampicillin/chloramphenicol
+
-
|{{todo|plate 3, pos. 60}}
+
-
|{{done|Sequence Okay!}}
+
-
|-
+
-
|High consitutive promoter(with RFP)
+
-
|Constitutive promoter
+
-
|<partinfo>Bba_J23100</partinfo>
+
-
|{{todo|Base Pairs (bp)}}
+
-
|<partinfo>pSB1AC3</partinfo>
+
-
|Ampicillin/chloramphenicol
+
-
|plate 3, pos. 75
+
-
|{{todo|Quality control}}
+
-
|-
+
-
|Medium consitutive promoter(with RFP)
+
-
|Constitutive promoter
+
-
|<partinfo>Bba_J23106</partinfo>
+
-
|{{todo|Base Pairs (bp)}}
+
-
|<partinfo>pSB1AC3</partinfo>
+
-
|Ampicillin/chloramphenicol
+
-
|plate 3, pos. 76
+
-
|{{todo|Quality control}}
+
-
|-
+
-
|Low consitutive promoter(with RFP)
+
-
|Constitutive promoter
+
-
|<partinfo>Bba_J23109</partinfo>
+
-
|{{todo|Base Pairs (bp)}}
+
-
|<partinfo>pSB1AC3</partinfo>
+
-
|Ampicillin/chloramphenicol
+
-
|plate 3, pos. 77
+
-
|{{todo|Quality control}}
+
-
|-
+
-
|High consitutive promoter(with RFP)
+
-
|Constitutive promoter
+
-
|<partinfo>Bba_J23100</partinfo>
+
-
|{{todo|Base Pairs (bp)}}
+
-
|<partinfo>pSB3K3</partinfo>
+
-
|Kanamycin
+
-
|plate 3, pos. 78
+
-
|{{todo|Quality control}}
+
-
|-
+
-
|Medium consitutive promoter(with RFP)
+
-
|Constitutive promoter
+
-
|<partinfo>Bba_J23106</partinfo>
+
-
|{{todo|Base Pairs (bp)}}
+
-
|<partinfo>pSB3K3</partinfo>
+
-
|Kanamycin
+
-
|plate 3, pos. 79
+
-
|{{todo|Quality control}}
+
-
|-
+
-
|Low consitutive promoter(with RFP)
+
-
|Constitutive promoter
+
-
|<partinfo>Bba_J23109</partinfo>
+
-
|{{todo|Base Pairs (bp)}}
+
-
|<partinfo>pSB3K3</partinfo>
+
-
|Kanamycin
+
-
|plate 3, pos. 80
+
-
|{{todo|Quality control}}
+
-
|-
+
-
|Reference constitutive promoter)
+
-
|Constitutive promoter
+
-
|?
+
-
|{{todo|Base Pairs (bp)}}
+
-
|?
+
-
|?
+
-
|plate 3, pos. 81
+
-
|{{todo|Quality control}}
+
-
|}
+

Latest revision as of 09:41, 22 January 2010

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Biobricks


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  • For a comprehensive list of all the parts we used, have a look at our parts registry.
  • Here you can find an informative overview of our submitted parts.
  • Experience we had with parts from the registry can be found under used parts.
  • Need anything specific? Take a look in our freezer