Team:Groningen/Parts

From 2009.igem.org

(Difference between revisions)
(Part List -80)
Line 26: Line 26:
|GVP
|GVP
|Gas Vesicle Proteins
|Gas Vesicle Proteins
-
|[http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
+
|<partinfo>BBa_I750016</partinfo>
|6064
|6064
-
|[http://partsregistry.org/Part:BBa_J61035 BBa_J61035]
+
|<partinfo>BBa_J61035</partinfo>
|Ampicillin/Gentamycin
|Ampicillin/Gentamycin
|plate 3, pos.1
|plate 3, pos.1
Line 35: Line 35:
|GVP (2nd batch)
|GVP (2nd batch)
|Gas Vesicle Proteins
|Gas Vesicle Proteins
-
|[http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
+
|<partinfo>BBa_I750016</partinfo>
|6064
|6064
-
|[http://partsregistry.org/Part:BBa_J61035 BBa_J61035]
+
|<partinfo>BBa_J61035</partinfo>
|Ampicillin/Gentamycin
|Ampicillin/Gentamycin
|plate 3, pos.2
|plate 3, pos.2
Line 98: Line 98:
|Promotor (low expression)
|Promotor (low expression)
|Constitutive promotor
|Constitutive promotor
-
|[http://partsregistry.org/Part:BBa_J23109 BBa_J23109]
+
|<partinfo>BBa_J23109</partinfo>
|35
|35
-
|[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
+
|<partinfo>BBa_J61002</partinfo>
|Ampicillin
|Ampicillin
|plate 3, pos.10
|plate 3, pos.10
Line 107: Line 107:
|Promotor (medium expression)
|Promotor (medium expression)
|Constitutive promotor
|Constitutive promotor
-
|[http://partsregistry.org/Part:BBa_J23106 BBa_J23106]
+
|<partinfo>BBa_J23106</partinfo>
|35
|35
-
|[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
+
|<partinfo>BBa_J61002</partinfo>
|Ampicillin
|Ampicillin
|plate 3, pos.11
|plate 3, pos.11
Line 116: Line 116:
|Promotor (high expression)
|Promotor (high expression)
|Constitutive promotor
|Constitutive promotor
-
|[http://partsregistry.org/Part:BBa_J23100 BBa_J23100]
+
|<partinfo>BBa_J23100</partinfo>
|35
|35
-
|[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
+
|<partinfo>BBa_J61002</partinfo>
|Ampicillin
|Ampicillin
|plate 3, pos.12
|plate 3, pos.12
Line 143: Line 143:
|RBS
|RBS
|Ribosomal Binding Site
|Ribosomal Binding Site
-
|[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]
+
|<partinfo>BBa_B0034</partinfo>
|12
|12
-
|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|<partinfo>pSB1A2</partinfo>
|Ampicillin
|Ampicillin
|plate 3, pos.19
|plate 3, pos.19
Line 151: Line 151:
|-  
|-  
|Terminator
|Terminator
-
|Double terminator consisting of [http://partsregistry.org/Part:BBa_B0012 BBa_B0012] and [http://partsregistry.org/Part:BBa_B0011 BBa_B0011]
+
|Double terminator consisting of <partinfo>BBa_B0012</partinfo> and <partinfo>BBa_B0011</partinfo>]
-
|[http://partsregistry.org/Part:BBa_B0014 BBa_B0014]
+
|<partinfo>BBa_B0014</partinfo>
|~90
|~90
-
|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
+
|<partinfo>pSB1AK3</partinfo>
|Ampicillin/Kanamycin
|Ampicillin/Kanamycin
|plate 3, pos.20
|plate 3, pos.20
Line 161: Line 161:
|Const. Pormoter + GVP
|Const. Pormoter + GVP
|Low constitutive promoter with GVP cluster
|Low constitutive promoter with GVP cluster
-
|[http://partsregistry.org/Part:BBa_J23109 BBa_J23109] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
+
|<partinfo>BBa_J23109</partinfo> + <partinfo>BBa_I750016</partinfo>
|~6100
|~6100
-
|[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
|plate 3, pos.21
|plate 3, pos.21
-
|EcoRI/PstI + {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}}
|-  
|-  
|Const. Pormoter + GVP (no.2)
|Const. Pormoter + GVP (no.2)
|Low constitutive promoter with GVP cluster
|Low constitutive promoter with GVP cluster
-
|[http://partsregistry.org/Part:BBa_J23109 BBa_J23109] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
+
|<partinfo>BBa_J23109</partinfo> + <partinfo>BBa_I750016</partinfo>
|~6100
|~6100
-
|[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
|plate 3, pos.22
|plate 3, pos.22
-
|EcoRI/PstI + {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}}
|-  
|-  
|Const. Pormoter + GVP
|Const. Pormoter + GVP
|Medium constitutive promoter with GVP cluster
|Medium constitutive promoter with GVP cluster
-
|[http://partsregistry.org/Part:BBa_J23106 BBa_J23106] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
+
|<partinfo>BBa_J23106</partinfo> + <partinfo>BBa_I750016</partinfo>
|~6100
|~6100
-
|[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
|plate 3, pos.23
|plate 3, pos.23
-
|EcoRI/PstI + {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}}
|-  
|-  
|Const. Pormoter + GVP (no.2)
|Const. Pormoter + GVP (no.2)
|Medium constitutive promoter with GVP cluster
|Medium constitutive promoter with GVP cluster
-
|[http://partsregistry.org/Part:BBa_J23106 BBa_J23106] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
+
|<partinfo>BBa_J23106</partinfo> + <partinfo>BBa_I750016</partinfo>
|~6100
|~6100
-
|[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
|plate 3, pos.24
|plate 3, pos.24
-
|EcoRI/PstI + {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}}
|-  
|-  
|HmtA (1.1)
|HmtA (1.1)
Line 217: Line 217:
|None
|None
|6149
|6149
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI)
+
|<partinfo>BBa_J61035</partinfo> (without the part between <i>Eco</i>RI and <i>Xba</i>I)
|Ampicillin
|Ampicillin
|plate 3, pos.37
|plate 3, pos.37
Line 226: Line 226:
|None
|None
|~6150
|~6150
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI)
+
|<partinfo>BBa_J61035</partinfo> (without the part between <i>Eco</i>RI and <i>Xba</i>I)
|Ampicillin
|Ampicillin
|plate 3, pos.38
|plate 3, pos.38
Line 235: Line 235:
|None
|None
|~6150
|~6150
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI)
+
|<partinfo>BBa_J61035</partinfo> (without the part between <i>Eco</i>RI and <i>Xba</i>I)
|Ampicillin
|Ampicillin
|plate 3, pos.39
|plate 3, pos.39
Line 242: Line 242:
|ArsR- in J61002
|ArsR- in J61002
|pArsR (promoter without RBS)
|pArsR (promoter without RBS)
-
|BBa_K190015
+
|<partinfo>BBa_K190015</partinfo>
|71
|71
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
+
|<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
|Ampicillin
|Ampicillin
|plate 3, pos.40
|plate 3, pos.40
-
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
|-  
|-  
|ZntR- in J61002
|ZntR- in J61002
|pZntR (promoter without RBS)
|pZntR (promoter without RBS)
-
|BBa_K190016
+
|<partinfo>BBa_K190016</partinfo>
|65
|65
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
+
|<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
|Ampicillin
|Ampicillin
|plate 3, pos.41
|plate 3, pos.41
-
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced (3 mutations)}}
+
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced (3 mutations)}}
|-  
|-  
|CueO- in J61002
|CueO- in J61002
|pCueO (promoter without RBS)
|pCueO (promoter without RBS)
-
|BBa_K190017
+
|<partinfo>BBa_K190017</partinfo>
|43
|43
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
+
|<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
|Ampicillin
|Ampicillin
|plate 3, pos.42
|plate 3, pos.42
-
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
|-  
|-  
|ZntR+RBS in J61002
|ZntR+RBS in J61002
|pZntR (promoter with own RBS)
|pZntR (promoter with own RBS)
-
|BBa_K190022
+
|<partinfo>BBa_K190022</partinfo>
|91
|91
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
+
|<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
|Ampicillin
|Ampicillin
|plate 3, pos.43
|plate 3, pos.43
-
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
|-  
|-  
|ArsR+RBS in J61002
|ArsR+RBS in J61002
|pArsR (promoter with own RBS)
|pArsR (promoter with own RBS)
-
|BBa_K190023
+
|<partinfo>BBa_K190023</partinfo>
|81
|81
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
+
|<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
|Ampicillin
|Ampicillin
|plate 3, pos.44
|plate 3, pos.44
-
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
|-  
|-  
|CueO+RBS in J61002
|CueO+RBS in J61002
|pCueO (promoter with own RBS)
|pCueO (promoter with own RBS)
-
|BBa_K190024
+
|<partinfo>BBa_K190024</partinfo>
|71
|71
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
|Ampicillin
|Ampicillin
|plate 3, pos.45
|plate 3, pos.45
-
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
|-
|-
|SmtA-GST in pGEX-3x
|SmtA-GST in pGEX-3x
Line 323: Line 323:
|GlpF and fMT in pMAL-MT
|GlpF and fMT in pMAL-MT
|GlpF and fMT on a synthetic operon in the vector
|GlpF and fMT on a synthetic operon in the vector
-
|<partinfo>BBa_K190019</partinfo> and BBa_K1900?? (GlpF has no BioBrick nr yet)
+
|<partinfo>BBa_K190019</partinfo> and <partinfo>BBa_K190028</partinfo>
|fMT = 200bp, GlpF = 900bp?
|fMT = 200bp, GlpF = 900bp?
|Ampicillin
|Ampicillin
Line 332: Line 332:
|MBP-ArsR
|MBP-ArsR
|MBP-ArsR Fusion protein
|MBP-ArsR Fusion protein
-
|{{todo|}}
+
|<partinfo>BBa_K190027</partinfo>
-
|1500 bp
+
|15180 bp
-
|pSB1AC3
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 53
|plate 3, pos. 53
Line 350: Line 350:
|pLac-fMT
|pLac-fMT
|Metallothionein with inducible promotor
|Metallothionein with inducible promotor
-
|{{todo|Part or Accession #}}
+
|<partinfo>BBa_K190032</partinfo>
-
|{{todo|Base Pairs (bp)}}
+
|430 bp
-
|pSB1AC3
+
|<partinfo>pSB1A2</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 55
|plate 3, pos. 55
Line 359: Line 359:
|pLow-fMT #4
|pLow-fMT #4
|Metallothionein with low constitutive promotor
|Metallothionein with low constitutive promotor
-
|{{todo|Part or Accession #}}
+
|<partinfo>BBa_K190031</partinfo>
-
|{{todo|Base Pairs (bp)}}
+
|265 bp
-
|pSB1A2
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 56
|plate 3, pos. 56
Line 368: Line 368:
|pLow-fMT #3
|pLow-fMT #3
|Metallothionein with low constitutive promotor
|Metallothionein with low constitutive promotor
-
|{{todo|Part or Accession #}}
+
|<partinfo>BBa_K190031</partinfo>
-
|{{todo|Base Pairs (bp)}}
+
|265 bp
-
|pSB1AC3
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 57
|plate 3, pos. 57
Line 377: Line 377:
|SmtA
|SmtA
|Metallothionein
|Metallothionein
-
|{{todo|Part or Accession #}}
+
|<partinfo>BBa_K190021</partinfo>
-
|{{todo|Base Pairs (bp)}}
+
|678 bp
-
|pSB1AC3
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 58
|plate 3, pos. 58
Line 386: Line 386:
|fMT #6
|fMT #6
|Metallothionein for As<sup>3+</sup>
|Metallothionein for As<sup>3+</sup>
-
|{{todo|Part or Accession #}}
+
|<partinfo>BBa_K190019</partinfo>
-
|{{todo|Base Pairs (bp)}}
+
|222 bp
-
|pSB1AC3
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 59
|plate 3, pos. 59
-
|{{done|Sequence 2 deletions between EcoRI and XbaI sites}}
+
|{{todo|Sequence 2 deletions between <i>Eco</i>RI and <i>Xba</i>I sites}}
 +
|-
 +
|fMT #4
 +
|Metallothionein for As<sup>3+</sup>
 +
|<partinfo>BBa_K190019</partinfo>
 +
| 222 bp
 +
|<partinfo>pSB1AC3</partinfo>
 +
|Ampicillin/chloramphenicol
 +
|{{todo|plate 3, pos. 60}}
 +
|{{done|Sequence Okay!}}
|-
|-
|High consitutive promoter(with RFP)
|High consitutive promoter(with RFP)
|Constitutive promoter
|Constitutive promoter
-
|Bba_J23100
+
|<partinfo>Bba_J23100</partinfo>
|{{todo|Base Pairs (bp)}}
|{{todo|Base Pairs (bp)}}
-
|pSB1AC3
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 75
|plate 3, pos. 75
Line 404: Line 413:
|Medium consitutive promoter(with RFP)
|Medium consitutive promoter(with RFP)
|Constitutive promoter
|Constitutive promoter
-
|Bba_J23106
+
|<partinfo>Bba_J23106</partinfo>
|{{todo|Base Pairs (bp)}}
|{{todo|Base Pairs (bp)}}
-
|pSB1AC3
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 76
|plate 3, pos. 76
Line 413: Line 422:
|Low consitutive promoter(with RFP)
|Low consitutive promoter(with RFP)
|Constitutive promoter
|Constitutive promoter
-
|Bba_J23109
+
|<partinfo>Bba_J23109</partinfo>
|{{todo|Base Pairs (bp)}}
|{{todo|Base Pairs (bp)}}
-
|pSB1AC3
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 77
|plate 3, pos. 77
Line 422: Line 431:
|High consitutive promoter(with RFP)
|High consitutive promoter(with RFP)
|Constitutive promoter
|Constitutive promoter
-
|Bba_J23100
+
|<partinfo>Bba_J23100</partinfo>
|{{todo|Base Pairs (bp)}}
|{{todo|Base Pairs (bp)}}
-
|pSB3K3
+
|<partinfo>pSB3K3</partinfo>
|Kanamycin
|Kanamycin
|plate 3, pos. 78
|plate 3, pos. 78
Line 431: Line 440:
|Medium consitutive promoter(with RFP)
|Medium consitutive promoter(with RFP)
|Constitutive promoter
|Constitutive promoter
-
|Bba_J23106
+
|<partinfo>Bba_J23106</partinfo>
|{{todo|Base Pairs (bp)}}
|{{todo|Base Pairs (bp)}}
-
|pSB3K3
+
|<partinfo>pSB3K3</partinfo>
|Kanamycin
|Kanamycin
|plate 3, pos. 79
|plate 3, pos. 79
Line 440: Line 449:
|Low consitutive promoter(with RFP)
|Low consitutive promoter(with RFP)
|Constitutive promoter
|Constitutive promoter
-
|Bba_J23109
+
|<partinfo>Bba_J23109</partinfo>
|{{todo|Base Pairs (bp)}}
|{{todo|Base Pairs (bp)}}
-
|pSB3K3
+
|<partinfo>pSB3K3</partinfo>
|Kanamycin
|Kanamycin
|plate 3, pos. 80
|plate 3, pos. 80

Revision as of 08:10, 4 September 2009

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Part List -80

Component Description Part or Accession # Base Pairs (bp) Plasmid (backbone) Resistance Well Quality control
GVP Gas Vesicle Proteins BBa_I750016 6064 BBa_J61035 Ampicillin/Gentamycin plate 3, pos.1 ?
GVP (2nd batch) Gas Vesicle Proteins BBa_I750016 6064 BBa_J61035 Ampicillin/Gentamycin plate 3, pos.2 ?
pHigh in low copy nr vector High consitutive promoter BBa_J23100 35bp pSB3K3 Kanamycin Plate 3, pos. 4 PCR(?), restriction(+)
pMed in low copy nr vector Medium consitutive promoter BBa_J23106 35bp pSB3K3 Kanamycin Plate 3, pos. 5 PCR(?), restriction(+)
pLow in low copy nr vector Low consitutive promoter BBa_J23109 35bp pSB3K3 Kanamycin Plate 3, pos. 6 PCR(?), restriction(+)
pHigh in high copy nr vector High consitutive promoter BBa_J23100 35bp pSB1AC3 Ampicillin/Chloramphenicol Plate 3, pos. 7 PCR(?), restriction(+)
pMed in high copy nr vector Medium consitutive promoter BBa_J23106 35bp pSB1AC3 Ampicillin/Chloramphenicol Plate 3, pos. 8 PCR(?), restriction(+)
pLow in high copy nr vector High consitutive promoter BBa_J23109 35bp pSB1AC3 Ampicillin/Chloramphenicol Plate 3, pos. 9 PCR(?), restriction(+)
Promotor (low expression) Constitutive promotor BBa_J23109 35 BBa_J61002 Ampicillin plate 3, pos.10 No
Promotor (medium expression) Constitutive promotor BBa_J23106 35 BBa_J61002 Ampicillin plate 3, pos.11 No
Promotor (high expression) Constitutive promotor BBa_J23100 35 BBa_J61002 Ampicillin plate 3, pos.12 No
Inducible promoter Lactose inducible promoter nr1 BBa_R0010 200bp pSB1A2 Ampicillin Plate 3, pos. 14 PCR(?), restriction(+)
Inducible promoter Lactose inducible promoter nr2 BBa_R0010 200bp pSB1A2 Ampicillin Plate 3, pos. 15 PCR(?), restriction(+)
RBS Ribosomal Binding Site BBa_B0034 12 pSB1A2 Ampicillin plate 3, pos.19 No
Terminator Double terminator consisting of BBa_B0012 and BBa_B0011] BBa_B0014 ~90 pSB1AK3 Ampicillin/Kanamycin plate 3, pos.20 No
Const. Pormoter + GVP Low constitutive promoter with GVP cluster BBa_J23109 + BBa_I750016 ~6100 pSB1AC3 Ampicillin/Chloramphenicol plate 3, pos.21 EcoRI/PstI + Sequenced Okay
Const. Pormoter + GVP (no.2) Low constitutive promoter with GVP cluster BBa_J23109 + BBa_I750016 ~6100 pSB1AC3 Ampicillin/Chloramphenicol plate 3, pos.22 EcoRI/PstI + Sequenced Okay
Const. Pormoter + GVP Medium constitutive promoter with GVP cluster BBa_J23106 + BBa_I750016 ~6100 pSB1AC3 Ampicillin/Chloramphenicol plate 3, pos.23 EcoRI/PstI + Sequenced Okay
Const. Pormoter + GVP (no.2) Medium constitutive promoter with GVP cluster BBa_J23106 + BBa_I750016 ~6100 pSB1AC3 Ampicillin/Chloramphenicol plate 3, pos.24 EcoRI/PstI + Sequenced Okay
HmtA (1.1) HmtA-His in pBAD(a) None ? None Ampicillin plate 3, pos.28 Restriction(+)
HmtA (2.1) HmtA-His in pBAD(a) None ? None Ampicillin plate 3, pos.29 Restriction(+)
CueO- + GVP in J61035 CueO promoter without RBS in front of the GVP cluster None 6149 BBa_J61035 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.37 Restriction(+) + Sequenced Okay
ZntR- + GVP in J61035 ZntR promoter without RBS in front of the GVP cluster None ~6150 BBa_J61035 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.38 Restriction(+) + Sequenced (3 mutations)
ArsR- + GVP in J61035 ArsR promoter without RBS in front of the GVP cluster None ~6150 BBa_J61035 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.39 Restriction(+) + Sequenced Okay
ArsR- in J61002 pArsR (promoter without RBS) BBa_K190015 71 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.40 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ZntR- in J61002 pZntR (promoter without RBS) BBa_K190016 65 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.41 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced (3 mutations)
CueO- in J61002 pCueO (promoter without RBS) BBa_K190017 43 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.42 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ZntR+RBS in J61002 pZntR (promoter with own RBS) BBa_K190022 91 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.43 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ArsR+RBS in J61002 pArsR (promoter with own RBS) BBa_K190023 81 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.44 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
CueO+RBS in J61002 pCueO (promoter with own RBS) BBa_K190024 71 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.45 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
SmtA-GST in pGEX-3x SmtA-GST fusion no biobrick primers available 1400bp pGEX-3x (NEB) Ampicillin plate 3, pos. 46 PCR, restriction
SmtA in pET29a SmtA BBa_K190021 900bp pET29a (NEB) Kanamycin plate 3, pos. 47 PCR, restriction
MymT in pGB68 MymT BBa_K190020 215bp Ampicillin pTXB1 (NEB) plate 3, pos. 48 PCR, restriction
GlpF and fMT in pMAL-MT GlpF and fMT on a synthetic operon in the vector BBa_K190019 and BBa_K190028 fMT = 200bp, GlpF = 900bp? Ampicillin pMALc2x (NEB) plate 3, pos. 49 PCR, restriction
MBP-ArsR MBP-ArsR Fusion protein BBa_K190027 15180 bp pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 53 Restriction analysis] & PCR
pNL29 GVP Gene cluster 6000 bp pBluescriptIIKS Ampicillin plate 3, pos. 54 Buoyancy phenotype
pLac-fMT Metallothionein with inducible promotor BBa_K190032 430 bp pSB1A2 Ampicillin/chloramphenicol plate 3, pos. 55 Quality control
pLow-fMT #4 Metallothionein with low constitutive promotor BBa_K190031 265 bp pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 56 Quality control
pLow-fMT #3 Metallothionein with low constitutive promotor BBa_K190031 265 bp pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 57 Quality control
SmtA Metallothionein BBa_K190021 678 bp pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 58 Quality control
fMT #6 Metallothionein for As3+ BBa_K190019 222 bp pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 59 Sequence 2 deletions between EcoRI and XbaI sites
fMT #4 Metallothionein for As3+ BBa_K190019 222 bp pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 60 Sequence Okay!
High consitutive promoter(with RFP) Constitutive promoter BBa_J23100 Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 75 Quality control
Medium consitutive promoter(with RFP) Constitutive promoter BBa_J23106 Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 76 Quality control
Low consitutive promoter(with RFP) Constitutive promoter BBa_J23109 Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 77 Quality control
High consitutive promoter(with RFP) Constitutive promoter BBa_J23100 Base Pairs (bp) pSB3K3 Kanamycin plate 3, pos. 78 Quality control
Medium consitutive promoter(with RFP) Constitutive promoter BBa_J23106 Base Pairs (bp) pSB3K3 Kanamycin plate 3, pos. 79 Quality control
Low consitutive promoter(with RFP) Constitutive promoter BBa_J23109 Base Pairs (bp) pSB3K3 Kanamycin plate 3, pos. 80 Quality control
Reference constitutive promoter) Constitutive promoter ? Base Pairs (bp) ? ? plate 3, pos. 81 Quality control