Team:Groningen/Parts

From 2009.igem.org

(Difference between revisions)
(Part List -80)
Line 26: Line 26:
|GVP
|GVP
|Gas Vesicle Proteins
|Gas Vesicle Proteins
-
|[http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
+
|<partinfo>BBa_I750016</partinfo>
|6064
|6064
-
|[http://partsregistry.org/Part:BBa_J61035 BBa_J61035]
+
|<partinfo>BBa_J61035</partinfo>
|Ampicillin/Gentamycin
|Ampicillin/Gentamycin
|plate 3, pos.1
|plate 3, pos.1
Line 35: Line 35:
|GVP (2nd batch)
|GVP (2nd batch)
|Gas Vesicle Proteins
|Gas Vesicle Proteins
-
|[http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
+
|<partinfo>BBa_I750016</partinfo>
|6064
|6064
-
|[http://partsregistry.org/Part:BBa_J61035 BBa_J61035]
+
|<partinfo>BBa_J61035</partinfo>
|Ampicillin/Gentamycin
|Ampicillin/Gentamycin
|plate 3, pos.2
|plate 3, pos.2
Line 98: Line 98:
|Promotor (low expression)
|Promotor (low expression)
|Constitutive promotor
|Constitutive promotor
-
|[http://partsregistry.org/Part:BBa_J23109 BBa_J23109]
+
|<partinfo>BBa_J23109</partinfo>
|35
|35
-
|[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
+
|<partinfo>BBa_J61002</partinfo>
|Ampicillin
|Ampicillin
|plate 3, pos.10
|plate 3, pos.10
Line 107: Line 107:
|Promotor (medium expression)
|Promotor (medium expression)
|Constitutive promotor
|Constitutive promotor
-
|[http://partsregistry.org/Part:BBa_J23106 BBa_J23106]
+
|<partinfo>BBa_J23106</partinfo>
|35
|35
-
|[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
+
|<partinfo>BBa_J61002</partinfo>
|Ampicillin
|Ampicillin
|plate 3, pos.11
|plate 3, pos.11
Line 116: Line 116:
|Promotor (high expression)
|Promotor (high expression)
|Constitutive promotor
|Constitutive promotor
-
|[http://partsregistry.org/Part:BBa_J23100 BBa_J23100]
+
|<partinfo>BBa_J23100</partinfo>
|35
|35
-
|[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
+
|<partinfo>BBa_J61002</partinfo>
|Ampicillin
|Ampicillin
|plate 3, pos.12
|plate 3, pos.12
Line 143: Line 143:
|RBS
|RBS
|Ribosomal Binding Site
|Ribosomal Binding Site
-
|[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]
+
|<partinfo>BBa_B0034</partinfo>
|12
|12
-
|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
+
|<partinfo>pSB1A2</partinfo>
|Ampicillin
|Ampicillin
|plate 3, pos.19
|plate 3, pos.19
Line 151: Line 151:
|-  
|-  
|Terminator
|Terminator
-
|Double terminator consisting of [http://partsregistry.org/Part:BBa_B0012 BBa_B0012] and [http://partsregistry.org/Part:BBa_B0011 BBa_B0011]
+
|Double terminator consisting of <partinfo>BBa_B0012</partinfo> and <partinfo>BBa_B0011</partinfo>]
-
|[http://partsregistry.org/Part:BBa_B0014 BBa_B0014]
+
|<partinfo>BBa_B0014</partinfo>
|~90
|~90
-
|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
+
|<partinfo>pSB1AK3</partinfo>
|Ampicillin/Kanamycin
|Ampicillin/Kanamycin
|plate 3, pos.20
|plate 3, pos.20
Line 161: Line 161:
|Const. Pormoter + GVP
|Const. Pormoter + GVP
|Low constitutive promoter with GVP cluster
|Low constitutive promoter with GVP cluster
-
|[http://partsregistry.org/Part:BBa_J23109 BBa_J23109] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
+
|<partinfo>BBa_J23109</partinfo> + <partinfo>BBa_I750016</partinfo>
|~6100
|~6100
-
|[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
|plate 3, pos.21
|plate 3, pos.21
-
|EcoRI/PstI + {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}}
|-  
|-  
|Const. Pormoter + GVP (no.2)
|Const. Pormoter + GVP (no.2)
|Low constitutive promoter with GVP cluster
|Low constitutive promoter with GVP cluster
-
|[http://partsregistry.org/Part:BBa_J23109 BBa_J23109] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
+
|<partinfo>BBa_J23109</partinfo> + <partinfo>BBa_I750016</partinfo>
|~6100
|~6100
-
|[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
|plate 3, pos.22
|plate 3, pos.22
-
|EcoRI/PstI + {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}}
|-  
|-  
|Const. Pormoter + GVP
|Const. Pormoter + GVP
|Medium constitutive promoter with GVP cluster
|Medium constitutive promoter with GVP cluster
-
|[http://partsregistry.org/Part:BBa_J23106 BBa_J23106] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
+
|<partinfo>BBa_J23106</partinfo> + <partinfo>BBa_I750016</partinfo>
|~6100
|~6100
-
|[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
|plate 3, pos.23
|plate 3, pos.23
-
|EcoRI/PstI + {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}}
|-  
|-  
|Const. Pormoter + GVP (no.2)
|Const. Pormoter + GVP (no.2)
|Medium constitutive promoter with GVP cluster
|Medium constitutive promoter with GVP cluster
-
|[http://partsregistry.org/Part:BBa_J23106 BBa_J23106] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
+
|<partinfo>BBa_J23106</partinfo> + <partinfo>BBa_I750016</partinfo>
|~6100
|~6100
-
|[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
|plate 3, pos.24
|plate 3, pos.24
-
|EcoRI/PstI + {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}}
|-  
|-  
|HmtA (1.1)
|HmtA (1.1)
Line 217: Line 217:
|None
|None
|6149
|6149
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI)
+
|<partinfo>BBa_J61035</partinfo> (without the part between <i>Eco</i>RI and <i>Xba</i>I)
|Ampicillin
|Ampicillin
|plate 3, pos.37
|plate 3, pos.37
Line 226: Line 226:
|None
|None
|~6150
|~6150
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI)
+
|<partinfo>BBa_J61035</partinfo> (without the part between <i>Eco</i>RI and <i>Xba</i>I)
|Ampicillin
|Ampicillin
|plate 3, pos.38
|plate 3, pos.38
Line 235: Line 235:
|None
|None
|~6150
|~6150
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI)
+
|<partinfo>BBa_J61035</partinfo> (without the part between <i>Eco</i>RI and <i>Xba</i>I)
|Ampicillin
|Ampicillin
|plate 3, pos.39
|plate 3, pos.39
Line 242: Line 242:
|ArsR- in J61002
|ArsR- in J61002
|pArsR (promoter without RBS)
|pArsR (promoter without RBS)
-
|BBa_K190015
+
|<partinfo>BBa_K190015</partinfo>
|71
|71
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
+
|<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
|Ampicillin
|Ampicillin
|plate 3, pos.40
|plate 3, pos.40
-
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
|-  
|-  
|ZntR- in J61002
|ZntR- in J61002
|pZntR (promoter without RBS)
|pZntR (promoter without RBS)
-
|BBa_K190016
+
|<partinfo>BBa_K190016</partinfo>
|65
|65
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
+
|<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
|Ampicillin
|Ampicillin
|plate 3, pos.41
|plate 3, pos.41
-
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced (3 mutations)}}
+
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced (3 mutations)}}
|-  
|-  
|CueO- in J61002
|CueO- in J61002
|pCueO (promoter without RBS)
|pCueO (promoter without RBS)
-
|BBa_K190017
+
|<partinfo>BBa_K190017</partinfo>
|43
|43
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
+
|<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
|Ampicillin
|Ampicillin
|plate 3, pos.42
|plate 3, pos.42
-
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
|-  
|-  
|ZntR+RBS in J61002
|ZntR+RBS in J61002
|pZntR (promoter with own RBS)
|pZntR (promoter with own RBS)
-
|BBa_K190022
+
|<partinfo>BBa_K190022</partinfo>
|91
|91
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
+
|<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
|Ampicillin
|Ampicillin
|plate 3, pos.43
|plate 3, pos.43
-
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
|-  
|-  
|ArsR+RBS in J61002
|ArsR+RBS in J61002
|pArsR (promoter with own RBS)
|pArsR (promoter with own RBS)
-
|BBa_K190023
+
|<partinfo>BBa_K190023</partinfo>
|81
|81
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
+
|<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
|Ampicillin
|Ampicillin
|plate 3, pos.44
|plate 3, pos.44
-
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
|-  
|-  
|CueO+RBS in J61002
|CueO+RBS in J61002
|pCueO (promoter with own RBS)
|pCueO (promoter with own RBS)
-
|BBa_K190024
+
|<partinfo>BBa_K190024</partinfo>
|71
|71
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
|Ampicillin
|Ampicillin
|plate 3, pos.45
|plate 3, pos.45
-
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}}
+
|<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
|-
|-
|SmtA-GST in pGEX-3x
|SmtA-GST in pGEX-3x
Line 323: Line 323:
|GlpF and fMT in pMAL-MT
|GlpF and fMT in pMAL-MT
|GlpF and fMT on a synthetic operon in the vector
|GlpF and fMT on a synthetic operon in the vector
-
|<partinfo>BBa_K190019</partinfo> and BBa_K1900?? (GlpF has no BioBrick nr yet)
+
|<partinfo>BBa_K190019</partinfo> and <partinfo>BBa_K190028</partinfo>
|fMT = 200bp, GlpF = 900bp?
|fMT = 200bp, GlpF = 900bp?
|Ampicillin
|Ampicillin
Line 332: Line 332:
|MBP-ArsR
|MBP-ArsR
|MBP-ArsR Fusion protein
|MBP-ArsR Fusion protein
-
|{{todo|}}
+
|<partinfo>BBa_K190027</partinfo>
-
|1500 bp
+
|15180 bp
-
|pSB1AC3
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 53
|plate 3, pos. 53
Line 350: Line 350:
|pLac-fMT
|pLac-fMT
|Metallothionein with inducible promotor
|Metallothionein with inducible promotor
-
|{{todo|Part or Accession #}}
+
|<partinfo>BBa_K190032</partinfo>
-
|{{todo|Base Pairs (bp)}}
+
|430 bp
-
|pSB1AC3
+
|<partinfo>pSB1A2</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 55
|plate 3, pos. 55
Line 359: Line 359:
|pLow-fMT #4
|pLow-fMT #4
|Metallothionein with low constitutive promotor
|Metallothionein with low constitutive promotor
-
|{{todo|Part or Accession #}}
+
|<partinfo>BBa_K190031</partinfo>
-
|{{todo|Base Pairs (bp)}}
+
|265 bp
-
|pSB1A2
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 56
|plate 3, pos. 56
Line 368: Line 368:
|pLow-fMT #3
|pLow-fMT #3
|Metallothionein with low constitutive promotor
|Metallothionein with low constitutive promotor
-
|{{todo|Part or Accession #}}
+
|<partinfo>BBa_K190031</partinfo>
-
|{{todo|Base Pairs (bp)}}
+
|265 bp
-
|pSB1AC3
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 57
|plate 3, pos. 57
Line 377: Line 377:
|SmtA
|SmtA
|Metallothionein
|Metallothionein
-
|{{todo|Part or Accession #}}
+
|<partinfo>BBa_K190021</partinfo>
-
|{{todo|Base Pairs (bp)}}
+
|678 bp
-
|pSB1AC3
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 58
|plate 3, pos. 58
Line 386: Line 386:
|fMT #6
|fMT #6
|Metallothionein for As<sup>3+</sup>
|Metallothionein for As<sup>3+</sup>
-
|{{todo|Part or Accession #}}
+
|<partinfo>BBa_K190019</partinfo>
-
|{{todo|Base Pairs (bp)}}
+
|222 bp
-
|pSB1AC3
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 59
|plate 3, pos. 59
-
|{{done|Sequence 2 deletions between EcoRI and XbaI sites}}
+
|{{todo|Sequence 2 deletions between <i>Eco</i>RI and <i>Xba</i>I sites}}
 +
|-
 +
|fMT #4
 +
|Metallothionein for As<sup>3+</sup>
 +
|<partinfo>BBa_K190019</partinfo>
 +
| 222 bp
 +
|<partinfo>pSB1AC3</partinfo>
 +
|Ampicillin/chloramphenicol
 +
|{{todo|plate 3, pos. 60}}
 +
|{{done|Sequence Okay!}}
|-
|-
|High consitutive promoter(with RFP)
|High consitutive promoter(with RFP)
|Constitutive promoter
|Constitutive promoter
-
|Bba_J23100
+
|<partinfo>Bba_J23100</partinfo>
|{{todo|Base Pairs (bp)}}
|{{todo|Base Pairs (bp)}}
-
|pSB1AC3
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 75
|plate 3, pos. 75
Line 404: Line 413:
|Medium consitutive promoter(with RFP)
|Medium consitutive promoter(with RFP)
|Constitutive promoter
|Constitutive promoter
-
|Bba_J23106
+
|<partinfo>Bba_J23106</partinfo>
|{{todo|Base Pairs (bp)}}
|{{todo|Base Pairs (bp)}}
-
|pSB1AC3
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 76
|plate 3, pos. 76
Line 413: Line 422:
|Low consitutive promoter(with RFP)
|Low consitutive promoter(with RFP)
|Constitutive promoter
|Constitutive promoter
-
|Bba_J23109
+
|<partinfo>Bba_J23109</partinfo>
|{{todo|Base Pairs (bp)}}
|{{todo|Base Pairs (bp)}}
-
|pSB1AC3
+
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 77
|plate 3, pos. 77
Line 422: Line 431:
|High consitutive promoter(with RFP)
|High consitutive promoter(with RFP)
|Constitutive promoter
|Constitutive promoter
-
|Bba_J23100
+
|<partinfo>Bba_J23100</partinfo>
|{{todo|Base Pairs (bp)}}
|{{todo|Base Pairs (bp)}}
-
|pSB3K3
+
|<partinfo>pSB3K3</partinfo>
|Kanamycin
|Kanamycin
|plate 3, pos. 78
|plate 3, pos. 78
Line 431: Line 440:
|Medium consitutive promoter(with RFP)
|Medium consitutive promoter(with RFP)
|Constitutive promoter
|Constitutive promoter
-
|Bba_J23106
+
|<partinfo>Bba_J23106</partinfo>
|{{todo|Base Pairs (bp)}}
|{{todo|Base Pairs (bp)}}
-
|pSB3K3
+
|<partinfo>pSB3K3</partinfo>
|Kanamycin
|Kanamycin
|plate 3, pos. 79
|plate 3, pos. 79
Line 440: Line 449:
|Low consitutive promoter(with RFP)
|Low consitutive promoter(with RFP)
|Constitutive promoter
|Constitutive promoter
-
|Bba_J23109
+
|<partinfo>Bba_J23109</partinfo>
|{{todo|Base Pairs (bp)}}
|{{todo|Base Pairs (bp)}}
-
|pSB3K3
+
|<partinfo>pSB3K3</partinfo>
|Kanamycin
|Kanamycin
|plate 3, pos. 80
|plate 3, pos. 80

Revision as of 08:10, 4 September 2009

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Part List -80

Component Description Part or Accession # Base Pairs (bp) Plasmid (backbone) Resistance Well Quality control
GVP Gas Vesicle Proteins 6064 Ampicillin/Gentamycin plate 3, pos.1 ?
GVP (2nd batch) Gas Vesicle Proteins 6064 Ampicillin/Gentamycin plate 3, pos.2 ?
pHigh in low copy nr vector High consitutive promoter 35bp Kanamycin Plate 3, pos. 4 PCR(?), restriction(+)
pMed in low copy nr vector Medium consitutive promoter 35bp Kanamycin Plate 3, pos. 5 PCR(?), restriction(+)
pLow in low copy nr vector Low consitutive promoter 35bp Kanamycin Plate 3, pos. 6 PCR(?), restriction(+)
pHigh in high copy nr vector High consitutive promoter 35bp Ampicillin/Chloramphenicol Plate 3, pos. 7 PCR(?), restriction(+)
pMed in high copy nr vector Medium consitutive promoter 35bp Ampicillin/Chloramphenicol Plate 3, pos. 8 PCR(?), restriction(+)
pLow in high copy nr vector High consitutive promoter 35bp Ampicillin/Chloramphenicol Plate 3, pos. 9 PCR(?), restriction(+)
Promotor (low expression) Constitutive promotor 35 Ampicillin plate 3, pos.10 No
Promotor (medium expression) Constitutive promotor 35 Ampicillin plate 3, pos.11 No
Promotor (high expression) Constitutive promotor 35 Ampicillin plate 3, pos.12 No
Inducible promoter Lactose inducible promoter nr1 200bp Ampicillin Plate 3, pos. 14 PCR(?), restriction(+)
Inducible promoter Lactose inducible promoter nr2 200bp Ampicillin Plate 3, pos. 15 PCR(?), restriction(+)
RBS Ribosomal Binding Site 12 Ampicillin plate 3, pos.19 No
Terminator Double terminator consisting of and ] ~90 Ampicillin/Kanamycin plate 3, pos.20 No
Const. Pormoter + GVP Low constitutive promoter with GVP cluster + ~6100 Ampicillin/Chloramphenicol plate 3, pos.21 EcoRI/PstI + Sequenced Okay
Const. Pormoter + GVP (no.2) Low constitutive promoter with GVP cluster + ~6100 Ampicillin/Chloramphenicol plate 3, pos.22 EcoRI/PstI + Sequenced Okay
Const. Pormoter + GVP Medium constitutive promoter with GVP cluster + ~6100 Ampicillin/Chloramphenicol plate 3, pos.23 EcoRI/PstI + Sequenced Okay
Const. Pormoter + GVP (no.2) Medium constitutive promoter with GVP cluster + ~6100 Ampicillin/Chloramphenicol plate 3, pos.24 EcoRI/PstI + Sequenced Okay
HmtA (1.1) HmtA-His in pBAD(a) None ? None Ampicillin plate 3, pos.28 Restriction(+)
HmtA (2.1) HmtA-His in pBAD(a) None ? None Ampicillin plate 3, pos.29 Restriction(+)
CueO- + GVP in J61035 CueO promoter without RBS in front of the GVP cluster None 6149 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.37 Restriction(+) + Sequenced Okay
ZntR- + GVP in J61035 ZntR promoter without RBS in front of the GVP cluster None ~6150 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.38 Restriction(+) + Sequenced (3 mutations)
ArsR- + GVP in J61035 ArsR promoter without RBS in front of the GVP cluster None ~6150 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.39 Restriction(+) + Sequenced Okay
ArsR- in J61002 pArsR (promoter without RBS) 71 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.40 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ZntR- in J61002 pZntR (promoter without RBS) 65 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.41 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced (3 mutations)
CueO- in J61002 pCueO (promoter without RBS) 43 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.42 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ZntR+RBS in J61002 pZntR (promoter with own RBS) 91 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.43 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ArsR+RBS in J61002 pArsR (promoter with own RBS) 81 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.44 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
CueO+RBS in J61002 pCueO (promoter with own RBS) 71 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.45 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
SmtA-GST in pGEX-3x SmtA-GST fusion no biobrick primers available 1400bp pGEX-3x (NEB) Ampicillin plate 3, pos. 46 PCR, restriction
SmtA in pET29a SmtA 900bp pET29a (NEB) Kanamycin plate 3, pos. 47 PCR, restriction
MymT in pGB68 MymT 215bp Ampicillin pTXB1 (NEB) plate 3, pos. 48 PCR, restriction
GlpF and fMT in pMAL-MT GlpF and fMT on a synthetic operon in the vector and fMT = 200bp, GlpF = 900bp? Ampicillin pMALc2x (NEB) plate 3, pos. 49 PCR, restriction
MBP-ArsR MBP-ArsR Fusion protein 15180 bp Ampicillin/chloramphenicol plate 3, pos. 53 Restriction analysis] & PCR
pNL29 GVP Gene cluster 6000 bp pBluescriptIIKS Ampicillin plate 3, pos. 54 Buoyancy phenotype
pLac-fMT Metallothionein with inducible promotor 430 bp Ampicillin/chloramphenicol plate 3, pos. 55 Quality control
pLow-fMT #4 Metallothionein with low constitutive promotor 265 bp Ampicillin/chloramphenicol plate 3, pos. 56 Quality control
pLow-fMT #3 Metallothionein with low constitutive promotor 265 bp Ampicillin/chloramphenicol plate 3, pos. 57 Quality control
SmtA Metallothionein 678 bp Ampicillin/chloramphenicol plate 3, pos. 58 Quality control
fMT #6 Metallothionein for As3+ 222 bp Ampicillin/chloramphenicol plate 3, pos. 59 Sequence 2 deletions between EcoRI and XbaI sites
fMT #4 Metallothionein for As3+ 222 bp Ampicillin/chloramphenicol plate 3, pos. 60 Sequence Okay!
High consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Ampicillin/chloramphenicol plate 3, pos. 75 Quality control
Medium consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Ampicillin/chloramphenicol plate 3, pos. 76 Quality control
Low consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Ampicillin/chloramphenicol plate 3, pos. 77 Quality control
High consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Kanamycin plate 3, pos. 78 Quality control
Medium consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Kanamycin plate 3, pos. 79 Quality control
Low consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Kanamycin plate 3, pos. 80 Quality control
Reference constitutive promoter) Constitutive promoter ? Base Pairs (bp) ? ? plate 3, pos. 81 Quality control