Team:Groningen/Parts

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Biobricks


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If you choose to include a Parts Submitted to the Registry page, please list your parts here. This is not necessary but it may be a nice list to keep track of.

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Part List -80

Component Description Part or Accession # Base Pairs (bp) Plasmid (backbone) Resistance Well Quality control
GVP Gas Vesicle Proteins BBa_I750016 6064 BBa_J61035 Ampicillin/Gentamycin plate 3, pos.1 ?
GVP (2nd batch) Gas Vesicle Proteins BBa_I750016 6064 BBa_J61035 Ampicillin/Gentamycin plate 3, pos.2 ?
pHigh in low copy nr vector High consitutive promoter BBa_J23100 35bp pSB3K3 Kanamycin Plate 3, pos. 4 PCR(?), restriction(+)
pMed in low copy nr vector Medium consitutive promoter BBa_J23106 35bp pSB3K3 Kanamycin Plate 3, pos. 5 PCR(?), restriction(+)
pLow in low copy nr vector Low consitutive promoter BBa_J23109 35bp pSB3K3 Kanamycin Plate 3, pos. 6 PCR(?), restriction(+)
pHigh in high copy nr vector High consitutive promoter BBa_J23100 35bp pSB1AC3 Ampicillin/Chloramphenicol Plate 3, pos. 7 PCR(?), restriction(+)
pMed in high copy nr vector Medium consitutive promoter BBa_J23106 35bp pSB1AC3 Ampicillin/Chloramphenicol Plate 3, pos. 8 PCR(?), restriction(+)
pLow in high copy nr vector High consitutive promoter BBa_J23109 35bp pSB1AC3 Ampicillin/Chloramphenicol Plate 3, pos. 9 PCR(?), restriction(+)
Promotor (low expression) Constitutive promotor BBa_J23109 35 BBa_J61002 Ampicillin plate 3, pos.10 No
Promotor (medium expression) Constitutive promotor BBa_J23106 35 BBa_J61002 Ampicillin plate 3, pos.11 No
Promotor (high expression) Constitutive promotor BBa_J23100 35 BBa_J61002 Ampicillin plate 3, pos.12 No
Inducible promoter Lactose inducible promoter nr1 BBa_R0010 200bp pSB1A2 Ampicillin Plate 3, pos. 14 PCR(?), restriction(+)
Inducible promoter Lactose inducible promoter nr2 BBa_R0010 200bp pSB1A2 Ampicillin Plate 3, pos. 15 PCR(?), restriction(+)
RBS Ribosomal Binding Site BBa_B0034 12 pSB1A2 Ampicillin plate 3, pos.19 No
Terminator Double terminator consisting of BBa_B0012 and BBa_B0011 BBa_B0014 ~90 pSB1AK3 Ampicillin/Kanamycin plate 3, pos.20 No
Const. Pormoter + GVP Low constitutive promoter with GVP cluster BBa_J23109 + BBa_I750016 ~6100 pSB1AC3 Ampicillin/Chloramphenicol plate 3, pos.21 EcoRI/PstI + Sequenced
Const. Pormoter + GVP (no.2) Low constitutive promoter with GVP cluster BBa_J23109 + BBa_I750016 ~6100 pSB1AC3 Ampicillin/Chloramphenicol plate 3, pos.22 EcoRI/PstI + Sequenced
Const. Pormoter + GVP Medium constitutive promoter with GVP cluster BBa_J23106 + BBa_I750016 ~6100 pSB1AC3 Ampicillin/Chloramphenicol plate 3, pos.23 EcoRI/PstI + Sequenced
Const. Pormoter + GVP (no.2) Medium constitutive promoter with GVP cluster BBa_J23106 + BBa_I750016 ~6100 pSB1AC3 Ampicillin/Chloramphenicol plate 3, pos.24 EcoRI/PstI + Sequenced
HmtA (1.1) HmtA-His in pBAD(a) None ? None Ampicillin plate 3, pos.28 Restriction(+)
HmtA (2.1) HmtA-His in pBAD(a) None ? None Ampicillin plate 3, pos.29 Restriction(+)
CueO- + GVP in J61035 CueO promoter without RBS in front of the GVP cluster None 6149 BBa_J61035 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.37 Restriction(+) + Sequenced
ZntR- + GVP in J61035 ZntR promoter without RBS in front of the GVP cluster None ~6150 BBa_J61035 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.38 Restriction(+) + Sequenced (3 mutations)
ArsR- + GVP in J61035 ArsR promoter without RBS in front of the GVP cluster None ~6150 BBa_J61035 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.39 Restriction(+) + Sequenced
ArsR- in J61002 pArsR (promoter without RBS) BBa_K190015 71 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.40 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced
ZntR- in J61002 pZntR (promoter without RBS) BBa_K190016 65 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.41 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced (3 mutations)
CueO- in J61002 pCueO (promoter without RBS) BBa_K190017 43 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.42 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced
ZntR+RBS in J61002 pZntR (promoter with own RBS) BBa_K190022 91 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.43 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced
ArsR+RBS in J61002 pArsR (promoter with own RBS) BBa_K190023 81 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.44 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced
CueO+RBS in J61002 pCueO (promoter with own RBS) BBa_K190024 71 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.45 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced
SmtA-GST in pGEX-3x SmtA-GST fusion no biobrick primers available 1400bp pGEX-3x (NEB) Ampicillin plate 3, pos. 46 PCR, restriction
SmtA in pET29a SmtA BBa_K190021 900bp pET29a (NEB) Kanamycin plate 3, pos. 47 PCR, restriction
MymT in pGB68 MymT BBa_K190020 215bp Ampicillin pTXB1 (NEB) plate 3, pos. 48 PCR, restriction
GlpF and fMT in pMAL-MT GlpF and fMT on a synthetic operon in the vector BBa_K190019 and BBa_K1900?? (GlpF has no BioBrick nr yet) fMT = 200bp, GlpF = 900bp? Ampicillin pMALc2x (NEB) plate 3, pos. 49 PCR, restriction
MBP-ArsR MBP-ArsR Fusion protein 1500 bp pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 53 Restriction analysis] & PCR
pNL29 GVP Gene cluster 6000 bp pBluescriptIIKS Ampicillin plate 3, pos. 54 Buoyancy phenotype
pLac-fMT Metallothionein with inducible promotor Part or Accession # Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 55 Quality control
pLow-fMT #4 Metallothionein with low constitutive promotor Part or Accession # Base Pairs (bp) pSB1A2 Ampicillin/chloramphenicol plate 3, pos. 56 Quality control
pLow-fMT #3 Metallothionein with low constitutive promotor Part or Accession # Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 57 Quality control
SmtA Metallothionein Part or Accession # Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 58 Quality control
fMT #6 Metallothionein for As3+ Part or Accession # Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 59 Sequence Okay
High consitutive promoter(with RFP) Constitutive promoter Bba_J23100 Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 75 Quality control
Medium consitutive promoter(with RFP) Constitutive promoter Bba_J23106 Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 76 Quality control
Low consitutive promoter(with RFP) Constitutive promoter Bba_J23109 Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 77 Quality control
High consitutive promoter(with RFP) Constitutive promoter Bba_J23100 Base Pairs (bp) pSB3K3 Kanamycin plate 3, pos. 78 Quality control
Medium consitutive promoter(with RFP) Constitutive promoter Bba_J23106 Base Pairs (bp) pSB3K3 Kanamycin plate 3, pos. 79 Quality control
Low consitutive promoter(with RFP) Constitutive promoter Bba_J23109 Base Pairs (bp) pSB3K3 Kanamycin plate 3, pos. 80 Quality control
Reference constitutive promoter) Constitutive promoter ? Base Pairs (bp) ? ? plate 3, pos. 81 Quality control