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Team:Groningen/Protocols
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(New page: {{Team:Groningen/Header}} Category:Team:Groningen <h1>Protocols</h1> <h2>Cloning</h2> <h3>[http://openwetware.org/wiki/PCR PCR]</h3> <h3>Plasmid isolation</h3> <h3>Restriction</h3> <h3...)
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(New page: {{Team:Groningen/Header}} Category:Team:Groningen <h1>Protocols</h1> <h2>Cloning</h2> <h3>[http://openwetware.org/wiki/PCR PCR]</h3> <h3>Plasmid isolation</h3> <h3>Restriction</h3> <h3...)
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Revision as of 10:46, 17 October 2009
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Protocols
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Protocols
Cloning
PCR
Plasmid isolation
Restriction
Gel isolation
Annealing synthetic oligo’s<h3> <h3>Ligation
Making competent cells
Transformation
Quality control
Colony PCR
Restriction analysis
List of solutions
Antibiotics
Ampicillin
100 mg/ml Ampicillin (1000x) Stock
- 1 g of Ampicillin sodium salt in 10 mL of demiwater (or 50% EtOH)
- Add NaOH or KOH to allow the Ampicillin to dissolve
- Filter sterilize 0.2 μm filter and aliquot
- Store -20 °C
Chloramphenicol
Chloramphenicol (1000x) Stock
- 0.35 g in 10 mL 100% EtOH
- Filter sterilize 0.2 μm filter and aliquot
- Store -20 °C
Kanamycin
