"
Team:Groningen/Protocols
From 2009.igem.org
(Difference between revisions)
m (New page: {{Team:Groningen/Header}} Category:Team:Groningen <h1>Protocols</h1> <h2>Cloning</h2> <h3>[http://openwetware.org/wiki/PCR PCR]</h3> <h3>Plasmid isolation</h3> <h3>Restriction</h3> <h3...) |
m |
||
| Line 23: | Line 23: | ||
* Store -20 °C | * Store -20 °C | ||
<h3>[http://openwetware.org/wiki/Chloramphenicol Chloramphenicol]</h3> | <h3>[http://openwetware.org/wiki/Chloramphenicol Chloramphenicol]</h3> | ||
| - | Chloramphenicol (1000x) Stock | + | 35 mg/ml Chloramphenicol (1000x) Stock |
* 0.35 g in 10 mL 100% EtOH | * 0.35 g in 10 mL 100% EtOH | ||
* Filter sterilize 0.2 μm filter and aliquot | * Filter sterilize 0.2 μm filter and aliquot | ||
* Store -20 °C | * Store -20 °C | ||
<h3>[http://openwetware.org/wiki/Kanamycin Kanamycin]</h3> | <h3>[http://openwetware.org/wiki/Kanamycin Kanamycin]</h3> | ||
| - | + | 50 mg/ml Kanamycin(1000x) Stock | |
| + | * {{todo| g in 10 mL }} | ||
| + | * Filter sterilize 0.2 μm filter and aliquot | ||
| + | * Store -20 °C | ||
{{Team:Groningen/Footer}} | {{Team:Groningen/Footer}} | ||
Revision as of 11:27, 17 October 2009
 |
Protocols
|
|---|
Protocols
Cloning
PCR
Plasmid isolation
Restriction
Gel isolation
Annealing synthetic oligo’s<h3> <h3>Ligation
Making competent cells
Transformation
Quality control
Colony PCR
Restriction analysis
List of solutions
Antibiotics
Ampicillin
100 mg/ml Ampicillin (1000x) Stock
- 1 g of Ampicillin sodium salt in 10 mL of demiwater (or 50% EtOH)
- Add NaOH or KOH to allow the Ampicillin to dissolve
- Filter sterilize 0.2 μm filter and aliquot
- Store -20 °C
Chloramphenicol
35 mg/ml Chloramphenicol (1000x) Stock
- 0.35 g in 10 mL 100% EtOH
- Filter sterilize 0.2 μm filter and aliquot
- Store -20 °C
Kanamycin
50 mg/ml Kanamycin(1000x) Stock
- g in 10 mL
- Filter sterilize 0.2 μm filter and aliquot
- Store -20 °C
