Team:Harvard/Protocols

From 2009.igem.org

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<h2> the way we did stuff, everytime </h2>
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        <h2> Protocols </h2>
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      <a href="https://2009.igem.org/Team:Harvard/Bacteria-Yeast Communication">Bacteria Yeast Communication</a>
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<a href="http://74.125.93.132/search?q=cache:nsdCILcgZcQJ:www.stratagene.com/manuals/200518.pdf+stratagene+site+directed+mutagensis&cd=1&hl=en&ct=clnk&gl=us&client=firefox-a">Site directed mutagenesis</a> <br>
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<a href="http://www.neb.com/nebecomm/products/protocol64.asp>PCR amplification</a><br>
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<a href="http://www.neb.com/nebecomm/products/protocol2.asp"> Quick ligation </a><br>
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      <a href="https://2009.igem.org/Team:Harvard/Restriction Digest">Restriction Digest</a>
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      <a href="https://2009.igem.org/Team:Harvard/Colony PCR">Colony PCR</a>
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<a href="http://74.125.93.132/search?q=cache:D60LJ_yepbYJ:kirschner.med.harvard.edu/files/protocols/QIAGEN_QIAprepMiniprepKit_EN.pdf+quiagen+miniprep&cd=2&hl=en&ct=clnk&gl=us&client=firefox-a"> Miniprep</a> <br><a href="http://docs.google.com/gview?a=v&q=cache:YBOZok5AVokJ:www.virology.wisc.edu/acp/Plasmids/PlasmidFiles/Protocols.pdf+quiagen+maxiprep&hl=en&gl=us&pid=bl&srcid=ADGEESj2Vc0xg_muu5BVON57ZJq_ZMX667-nK3iHjoL4JDKHUhBAniGHFMLMUtV5MjrlJb3rtdJ4AC8GAruJ-PeiawAUFupobPi8s1Fs4MjqpPknqKvj3OGrUH0Whadl4uYMGuymYpsu&sig=AFQjCNHvA6VFrdNYLWD9D11U-XJsDAiLQQ">Maxiprep</a>
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<br><a href="http://docs.google.com/gview?a=v&q=cache:GXfsHKGeGEoJ:francois.schweisguth.free.fr/protocols/QIAquick_PCR_Purification_Kit.pdf+&hl=en&gl=us&sig=AFQjCNFNQ6nlQRMs9MSkat8-zFzlNNe1pg">PCR purification </a><br>
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<a href="http://74.125.93.132/search?q=cache:duYtZlhJBzsJ:www.biochem.uiowa.edu/donelson/Database%2520items/QIAQUICK%2520GEL%2520PURIFICATION.doc+gel+purification&cd=3&hl=en&ct=clnk&gl=us&client=firefox-a">Gel purification</a><br>
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<a href="https://2009.igem.org/Team:Harvard/Xgal">X Gal/ Beta Galactosidase Assay</a>
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     <ul>
     <ul>
       <li><a href="https://2009.igem.org/Team:Harvard/Comm">Bacteria-to-Yeast Communication</a></li>
       <li><a href="https://2009.igem.org/Team:Harvard/Comm">Bacteria-to-Yeast Communication</a></li>
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       <li><a href="https://2009.igem.org/Team:Harvard/Laser">Laser-petter</a></li>
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       <li><a href="https://2009.igem.org/Team:Harvard/Laser">Characterization</a></li>
       <li><a href="https://2009.igem.org/Team:Harvard/PCB">PCB Biosynthesis in Yeast</a></li>
       <li><a href="https://2009.igem.org/Team:Harvard/PCB">PCB Biosynthesis in Yeast</a></li>
       <li><a href="https://2009.igem.org/Team:Harvard/Split">Split Luciferase</a></li>
       <li><a href="https://2009.igem.org/Team:Harvard/Split">Split Luciferase</a></li>
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       <li><a href="https://2009.igem.org/Team:Harvard/Blackboard">Yeast Cellular Blackboard</a></li>
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       <li><a href="https://2009.igem.org/Team:Harvard/Blackboard">Other Possible Reporters</a></li>
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      <li><a href="https://2009.igem.org/Team:Harvard/Mating">Yeast Red Light District</a></li>
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<h2><p><a href="https://2009.igem.org/Team:Harvard/Parts">Parts</a></p></h2>
<h2><p><a href="https://2009.igem.org/Team:Harvard/Parts">Parts</a></p></h2>
         <h2><p><a href="https://2009.igem.org/Team:Harvard/References">References</a></p></h2>
         <h2><p><a href="https://2009.igem.org/Team:Harvard/References">References</a></p></h2>
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<h2><p><a href="https://2009.igem.org/Team:Harvard/Ethics">Ethics</a></h2>
<h2><p><a href="https://2009.igem.org/Team:Harvard/Ethics">Ethics</a></h2>
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        <h2><p><a href="https://2009.igem.org/Team:Harvard/Safety">Safety</a></h2>
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        <h2><p><a href="https://2009.igem.org/Team:Harvard/Acknowledgements">Acknowledgements</a></h2>
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Latest revision as of 03:34, 22 October 2009

Hi Mom

Protocols

/***********************************************************************************************************************/


Bacteria Yeast Communication
Site directed mutagenesis
Quick ligation
Restriction Digest
Colony PCR
Miniprep
Maxiprep
PCR purification
Gel purification
X Gal/ Beta Galactosidase Assay

Team

Projects

Parts

References

Lab Notebook

Ethics

Safety

Acknowledgements

Retrieved from "http://2009.igem.org/Team:Harvard/Protocols"