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Team:IBB Pune/protein-based-signalling
From 2009.igem.org
Contents |
The Complete Turing Machine
The above image is a representation of the complete turing machine including inter state regulation. This model is true to Alan Turing's vision of multi-mutually exclusive states and the states being regulated by one another through outputs.
Working of the Construct
In the above construct, there are three independent modules that function
- A constitutively working module which produces LuxR protein and LacI and Cassette 1 (encodes ogr and activates state 'B')
- A State-'B' which is activated by State' A' and which in turn represses the working of the default state
- An AND gate regulated activation of the turing machine function (it could be lysis, expression of a fluorescent protein or the synthesis of vanillin or methyl salicylate)
The default state of the turing machine is 'A'. In this state it encounters a '0' (represented by Lactose) first.However there are no Lactose responsive elements in either the first or the second module. The third Module which has such a module however also requires the presence of the phage protein 'ogr ' . Hence the first 0 is unable to produce any effect and the machine moves on to the next cell in the tape while remaining in state A.
The machine now encounters a 1 in this default state 'A' (represented by AHL). This results in the activation of the second module which in turn produces more of ogr. This acts like a positive feedback loop and hence state 'B' is switched on and can remain active without intervention from cassette A.
