Team:IIT Madras/Notebook/Sandbox

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Revision as of 12:27, 19 October 2009

Contents

Intermediates and proposed constructs for the the 2 plasmid locking system

Original design

In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).


2plasmidoriginaldesignplasmid1.jpg


Final design

We decided to remove the extra antibiotic markers from the inserts. Then it looks like this


File:2plasmidfinaldesign.jpg


We had more than one strategy to build the whole construct.


strategy 1

File:2plasmidstrategy1.jpg



strategy 2

File:2plasmidstrategy2.jpg


Intermediates and proposed constructs for the the 3 plasmid locking system

We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too.

intermediates planned through PCR protocol

Plasmids for a self locking assembly