Team:IIT Madras/Notebook/Sandbox

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== Intermediates and final constructs planned for the the 2 plasmid locking system ==
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== Intermediates and final constructs planned for the the 3 plasmid locking system ==
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== intermediates planned through PCR protocol ==
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== Plasmids for a self locking assembly ==
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<div id="aContent">
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</html>
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==Intermediates and proposed constructs for the the 2 plasmid locking system==
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==Original design==
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In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).
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<html><br></html>
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<b><font color="#000">Plasmid 1</font></b>
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<html><br></html>
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[[Image:2plasmidoriginaldesignplasmid1.jpg]]
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<html><br></html>
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<b><font color="#000">Plasmid 2</font></b>
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<html><br></html>
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[[Image:2plasmidoriginaldesignplasmid2.jpg]]
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<html><br></html>
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==Final design==
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We decided to remove the extra antibiotic markers from the inserts. Then it looks like this
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<html><br></html>
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<b><font color="#000">Plasmid 1</font></b>
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<html><br></html>
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We had more than one way to build plasmid 1
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<html><br></html>
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<b><font color="#000">Path 1</font></b>
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[[Image:2plasmidfinaldesignplasmid1path1.jpg]]
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<html><br></html>
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<b><font color="#000">Path 1</font></b>
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[[Image:2plasmidfinaldesignplasmid1path2.jpg]]
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<html><br></html>
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<b><font color="#000">Plasmid 2</font></b>
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<html><br></html>
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[[Image:2plasmidfinaldesignplasmid2.jpg]]
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<html><br></html>
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==Intermediates and proposed constructs for the the 3 plasmid locking system==
 +
 
 +
We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too.
 +
 
 +
== Intermediates planned through PCR protocol ==
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<html><br></html>
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These parts are essentially same as the those that occur in the construction strategies that are shown above.
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{{:Team:IITM/footer}}

Latest revision as of 01:30, 22 October 2009

Indian Institute of Technology,Madras

IIT Madras














Contents

Intermediates and proposed constructs for the the 2 plasmid locking system

Original design

In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).


Plasmid 1


2plasmidoriginaldesignplasmid1.jpg


Plasmid 2


2plasmidoriginaldesignplasmid2.jpg


Final design

We decided to remove the extra antibiotic markers from the inserts. Then it looks like this


Plasmid 1


We had more than one way to build plasmid 1


Path 1

2plasmidfinaldesignplasmid1path1.jpg


Path 1

2plasmidfinaldesignplasmid1path2.jpg


Plasmid 2


2plasmidfinaldesignplasmid2.jpg


Intermediates and proposed constructs for the the 3 plasmid locking system

We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too.

Intermediates planned through PCR protocol


These parts are essentially same as the those that occur in the construction strategies that are shown above.