Team:IIT Madras/Notebook/Sandbox

From 2009.igem.org

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(Intermediates and final constructs planned for the the 2 plasmid locking system)
(Intermediates planned through PCR protocol)
 
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== '''Intermediates and proposed constructs for the the 2 plasmid locking system''' ==
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<html>
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<br><br><br><br><br><br><br><br><br><br><br>
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<br><br>
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<head><style>
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== Original design ==
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body
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{
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background: #000;
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}
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#aContent
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{
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background-color:#fff;
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margin: 50px auto;
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padding: 10px 10px 60px 10px;
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width: 800px;
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color: #000;
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}
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</style>
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<STYLE TYPE="text/css">
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.justifyalign {text-align:justify}
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<body>
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<div id="aContent">
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</html>
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==Intermediates and proposed constructs for the the 2 plasmid locking system==
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==Original design==
In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).
In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).
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[[Image:2plasmidoriginaldesign.jpg]]
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<b><font color="#000">Plasmid 1</font></b>
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<html><br></html>
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[[Image:2plasmidoriginaldesignplasmid1.jpg]]
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<b><font color="#000">Plasmid 2</font></b>
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== Final design ==
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<html><br></html>
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[[Image:2plasmidoriginaldesignplasmid2.jpg]]
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<html><br></html>
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==Final design==
We decided to remove the extra antibiotic markers from the inserts. Then it looks like this
We decided to remove the extra antibiotic markers from the inserts. Then it looks like this
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[[Image:2plasmidfinaldesign.jpg]]
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<b><font color="#000">Plasmid 1</font></b>
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<html><br></html>
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We had more than one strategy to build the whole construct.
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We had more than one way to build plasmid 1
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<html><br></html>
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== ''strategy 1'' ==
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<b><font color="#000">Path 1</font></b>
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[[Image:2plasmidstrategy1.jpg]]
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[[Image:2plasmidfinaldesignplasmid1path1.jpg]]
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<html><br></html>
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<b><font color="#000">Path 1</font></b>
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== ''strategy 2'' ==
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[[Image:2plasmidfinaldesignplasmid1path2.jpg]]
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[[Image:2plasmidstrategy2.jpg]]
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<html><br></html>
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<b><font color="#000">Plasmid 2</font></b>
<html><br></html>
<html><br></html>
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== Intermediates and final constructs planned for the the 3 plasmid locking system ==
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[[Image:2plasmidfinaldesignplasmid2.jpg]]
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<html><br></html>
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==Intermediates and proposed constructs for the the 3 plasmid locking system==
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We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too.
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== Intermediates planned through PCR protocol ==
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<html><br></html>
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== intermediates planned through PCR protocol ==
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These parts are essentially same as the those that occur in the construction strategies that are shown above.
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== Plasmids for a self locking assembly ==
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{{:Team:IITM/footer}}

Latest revision as of 01:30, 22 October 2009

Indian Institute of Technology,Madras

IIT Madras














Contents

Intermediates and proposed constructs for the the 2 plasmid locking system

Original design

In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).


Plasmid 1


2plasmidoriginaldesignplasmid1.jpg


Plasmid 2


2plasmidoriginaldesignplasmid2.jpg


Final design

We decided to remove the extra antibiotic markers from the inserts. Then it looks like this


Plasmid 1


We had more than one way to build plasmid 1


Path 1

2plasmidfinaldesignplasmid1path1.jpg


Path 1

2plasmidfinaldesignplasmid1path2.jpg


Plasmid 2


2plasmidfinaldesignplasmid2.jpg


Intermediates and proposed constructs for the the 3 plasmid locking system

We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too.

Intermediates planned through PCR protocol


These parts are essentially same as the those that occur in the construction strategies that are shown above.