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Team:IIT Madras/Notebook/Sandbox
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| - | == | + | ==Intermediates and proposed constructs for the the 2 plasmid locking system== |
| - | == Original design == | + | ==Original design== |
In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker). | In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker). | ||
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| - | + | <b><font color="#000">Plasmid 1</font></b> | |
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| - | + | <b><font color="#000">Plasmid 2</font></b> | |
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| - | == Final design == | + | ==Final design== |
We decided to remove the extra antibiotic markers from the inserts. Then it looks like this | We decided to remove the extra antibiotic markers from the inserts. Then it looks like this | ||
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| - | + | <b><font color="#000">Plasmid 1</font></b> | |
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| - | + | <b><font color="#000">Path 1</font></b> | |
[[Image:2plasmidfinaldesignplasmid1path1.jpg]] | [[Image:2plasmidfinaldesignplasmid1path1.jpg]] | ||
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| - | + | <b><font color="#000">Path 1</font></b> | |
[[Image:2plasmidfinaldesignplasmid1path2.jpg]] | [[Image:2plasmidfinaldesignplasmid1path2.jpg]] | ||
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| - | + | <b><font color="#000">Plasmid 2</font></b> | |
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| - | == Intermediates and proposed constructs for the the 3 plasmid locking system == | + | ==Intermediates and proposed constructs for the the 3 plasmid locking system== |
We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too. | We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too. | ||
Latest revision as of 01:30, 22 October 2009
IIT Madras
Contents |
Intermediates and proposed constructs for the the 2 plasmid locking system
Original design
In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).
Plasmid 1
Plasmid 2
Final design
We decided to remove the extra antibiotic markers from the inserts. Then it looks like this
Plasmid 1
We had more than one way to build plasmid 1
Path 1
Path 1
Plasmid 2
Intermediates and proposed constructs for the the 3 plasmid locking system
We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too.
Intermediates planned through PCR protocol
These parts are essentially same as the those that occur in the construction strategies that are shown above.
