Team:IIT Madras/Notebook/Sandbox

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Contents

Intermediates and proposed constructs for the the 2 plasmid locking system

Original design

In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).


Plasmid 1


2plasmidoriginaldesignplasmid1.jpg


Final design

We decided to remove the extra antibiotic markers from the inserts. Then it looks like this


File:2plasmidfinaldesign.jpg


We had more than one strategy to build the whole construct.


strategy 1

File:2plasmidstrategy1.jpg



strategy 2

File:2plasmidstrategy2.jpg


Intermediates and proposed constructs for the the 3 plasmid locking system

We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too.

intermediates planned through PCR protocol

Plasmids for a self locking assembly