Team:IIT Madras/Results

From 2009.igem.org

(Difference between revisions)
Line 106: Line 106:
<html>
<html>
-
For updated results, keep checking <a href="https://2009.igem.org/User:Abdul">here.</a>
+
For updated results, keep checking <a href="http://openwetware.org/wiki/IGEM:Indian_Institue_of_Technology_Madras/2009/Notebook/PLASMID_-_Plasmid_Locking_Assembly_for_Sustaining_Multiple_Insert_DNA/2009/10/31">here.</a>
</html>
</html>
===Fluorescent Imaging===
===Fluorescent Imaging===
Line 112: Line 112:
<html>
<html>
-
For updated results, keep checking <a href="https://2009.igem.org/User:Abdul">here.</a>
+
For updated results, keep checking <a href="http://openwetware.org/wiki/IGEM:Indian_Institue_of_Technology_Madras/2009/Notebook/PLASMID_-_Plasmid_Locking_Assembly_for_Sustaining_Multiple_Insert_DNA/2009/10/31">here.</a>
</html>
</html>
{{:Team:IITM/footer}}
{{:Team:IITM/footer}}

Revision as of 17:34, 21 October 2009

Indian Institute of Technology,Madras

IIT Madras













Contents

Results

Comparing the differences in the growth rates of cells with and without plasmids in various media



Growth curves


DH5aall4cases.jpg
Fig 8.1: DH5a grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.


Rfpall4cases.jpg
Fig 8.2: RFP (pSB1C3) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.


Cfpall4cases.jpg
Fig 8.3: CFP (pSB1A2) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.



RC4cases.jpg
Fig 8.4: RFP (pSB1C3) - CFP (pSB1A2) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.



Noaball4.jpg
Fig 8.5: All 4 strains in media without antibiotics.



Corraball4.jpg
Fig 8.6: All 4 strains in their corresponding antibiotic media - DH5a in Lb without antibiotics, RFP (pSB1C3) in LB with Chloramphenicol, CFP (pSB1A2) in LB with Ampicillin, RFP (pSB1C3) - CFP (pSB1A2) cells in LB with Chloramphenicol and Ampicillin.



Logall4noab.jpg
Fig 8.7: log (OD600) vs Time plot for all 4 strains in media without antibiotics.



Logcorrab.jpg
Fig 8.8: log (OD600) vs Time plot for all 4 strains in their corresponding antibiotic media - DH5a in Lb without antibiotics, RFP (pSB1C3) in LB with Chloramphenicol, CFP (pSB1A2) in LB with Ampicillin, RFP (pSB1C3) - CFP (pSB1A2) cells in LB with Chloramphenicol and Ampicillin.

Modeling

For updated results, keep checking here.

Fluorescent Imaging

For updated results, keep checking here.