Team:IPN-UNAM-Mexico/Notebook

From 2009.igem.org

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Revision as of 07:21, 21 October 2009


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Contents

As summer proyect our experimental work began at july the 7th 2009 using 2008 bioparts catalog, unfortunately it didn't work properly, so we had a lot of trouble to take out DNA and transform into E. coli. For this reason we had to delay lab work and request the 2009 catalog.

Month-icon.pngProtocols

Month-icon.pngJuly

Month-icon.pngAugust

Month-icon.pngSeptember

Month-icon.pngOctober

Our synthetic construction consists of 29 bioparts, which needed 12 ligations and different strategies to make it functional as is described below:

Ligation Nickname Biobrick
4-23 Ciencias I BBa_K226005
13-15 Ciencias II BBa_K266002
Ciencias I-7 Ciencias III BBa_K266006
24-17 Ciencias IV BBa_K266001
2-3 Ciencias V BBa_K266000
J231000-11 Ciencias VI BBa_K266008
Amplicon - Ciencias II Ciencias VII BBa_K266003
Ciencias VI - 12 Ciencias VIII BBa_K266009
Ciencias III - 19 Ciencias IX BBa_K266006
J23100 – Ciencias VII Ciencias X BBa_K266004
Ciencias III - Ciencias V Ciencias XI BBa_K266007
Ciencias X - Ciencias IV Ciencias XII BBa_K266010

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