Team:IPN-UNAM-Mexico/Notebook/October

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==='''''05-October-2009'''''===
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We going to digest L2-3 (BBa_K266000) as insert XBAI and PSTI 17 hours digest in 37°C camera.
 +
 +
----
 +
----
 +
 +
==='''''06-October-2009'''''===
 +
 +
The digest L2-3(BBa_K266000) dosen´t work, we have to repeat it.
 +
Left 17 hours gigest.
 +
----
 +
----
 +
 +
==='''''07-October-2009'''''===
 +
 +
We run out the digest and it is successful. Next step is attach to (BBa_K266006) as backbone.
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Left  17 hours ligation overnight.
 +
----
 +
----
 +
 +
==='''''08-October-2009'''''===
 +
 +
Transformation  by head shock, plate and left overnight in 37°C camera.
 +
----
 +
----
 +
 +
==='''''09-October-2009'''''===
 +
 +
The transformation was successful we get five colonies.
 +
----
 +
----
 +
 +
==='''''12-October-2009'''''===
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 +
Left two colonies in LB medium inoculums overnight to prepare midi tomorrow.
 +
----
 +
----
 +
 +
==='''''13-October-2009'''''===
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 +
We have culture and prepare midi prep, with this we complete.
 +
----
 +
----
 +
 +
==='''''15-October-2009'''''===
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 +
Biobricks sent to the registry and final ligation in progress
 +
----
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----
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Revision as of 23:33, 21 October 2009


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Contents

05-October-2009

We going to digest L2-3 (BBa_K266000) as insert XBAI and PSTI 17 hours digest in 37°C camera.



06-October-2009

The digest L2-3(BBa_K266000) dosen´t work, we have to repeat it. Left 17 hours gigest.



07-October-2009

We run out the digest and it is successful. Next step is attach to (BBa_K266006) as backbone. Left 17 hours ligation overnight.



08-October-2009

Transformation by head shock, plate and left overnight in 37°C camera.



09-October-2009

The transformation was successful we get five colonies.



12-October-2009

Left two colonies in LB medium inoculums overnight to prepare midi tomorrow.



13-October-2009

We have culture and prepare midi prep, with this we complete.



15-October-2009

Biobricks sent to the registry and final ligation in progress



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