Team:IPN-UNAM-Mexico/Notebook/October

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Contents

05-October-2009

We going to digest L2-3 (BBa_K266000) as insert XBAI and PSTI 17 hours digest in 37°C camera.



06-October-2009

The digest L2-3(BBa_K266000) doesn´t work, we have to repeat it. Left 17 hours digest. Ligacion 2- 3.jpg



07-October-2009

We run out the digest and it is successful. Next step is attach to (BBa_K266006) as backbone. Left 17 hours ligation overnight.



08-October-2009

Transformation by head shock, plate and left overnight in 37ºC.



09-October-2009

The transformation was successful we get five colonies.



12-October-2009

Left two colonies in LB medium inoculums overnight to prepare midi tomorrow.



13-October-2009

We have culture and prepare midi prep.



15-October-2009

Biobricks sent to the registry and final ligation in progress.



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