http://2009.igem.org/wiki/index.php?title=Team:IPN-UNAM-Mexico/Protocols&feed=atom&action=historyTeam:IPN-UNAM-Mexico/Protocols - Revision history2024-03-19T12:07:52ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:IPN-UNAM-Mexico/Protocols&diff=168955&oldid=prevCjdg at 03:51, 22 October 20092009-10-22T03:51:56Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Wash with EtOH at 70% and centrifugate 2 min at 13000 rpm, let the pellet dry and suspend on 500 ml of esterile deionized water (Water for pcr).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Wash with EtOH at 70% and centrifugate 2 min at 13000 rpm, let the pellet dry and suspend on 500 ml of esterile deionized water (Water for pcr).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>== <del class="diffchange diffchange-inline">[[Image:Month-icon.png | 50px]] </del>Competent cells with RbCl ==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>== Competent cells with RbCl ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Take 5 ml of liquid SOB and incubate at 37 °C overnight</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Take 5 ml of liquid SOB and incubate at 37 °C overnight</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Take 1 ml of cell culture and innoculate into 500 ml of YENB media and incubate at 37 °C and 200 rpm until O.D.=0.5-0.55</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Take 1 ml of cell culture and innoculate into 500 ml of YENB media and incubate at 37 °C and 200 rpm until O.D.=0.5-0.55</div></td></tr>
</table>Cjdghttp://2009.igem.org/wiki/index.php?title=Team:IPN-UNAM-Mexico/Protocols&diff=168925&oldid=prevCjdg at 03:51, 22 October 20092009-10-22T03:51:02Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Adjust pH to 6.8 with 0.2 M of NaOH and sterilize by filtration using a 0.22 μm filter</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Adjust pH to 6.8 with 0.2 M of NaOH and sterilize by filtration using a 0.22 μm filter</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==<del class="diffchange diffchange-inline">[[Image:Month-icon.png | 50px]] </del>CTAB miniprep==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==CTAB miniprep==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Take 1.5 ml of cell culture and centrifuge at 15000 rpm for 3 minutes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Take 1.5 ml of cell culture and centrifuge at 15000 rpm for 3 minutes.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Discard liquid phase</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Discard liquid phase</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Incubate at 37°C for 30 min.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Incubate at 37°C for 30 min.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>== <del class="diffchange diffchange-inline">[[Image:Month-icon.png | 50px]] </del>DNA purification from agarose gel ==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>== <ins class="diffchange diffchange-inline"> </ins>DNA purification from agarose gel ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#In a UV room, cut the gel with a knife and place DNA bands inside falcon tubes</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#In a UV room, cut the gel with a knife and place DNA bands inside falcon tubes</div></td></tr>
</table>Cjdghttp://2009.igem.org/wiki/index.php?title=Team:IPN-UNAM-Mexico/Protocols&diff=166514&oldid=prevCjdg at 02:49, 22 October 20092009-10-22T02:49:55Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Protocols=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Protocols=</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== MIDIPREP ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== MIDIPREP ==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Francisco Razo Hernández (noviembre 2008). </del>Modified from “QIagen Plasmid Purification Midi”.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Modified from “QIagen Plasmid Purification Midi”.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Inoculate a colony in 25 - 50 ml of LB Medium with antibiotic. For low copy plasmids, inoculate 100 - 200 ml of medium.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Inoculate a colony in 25 - 50 ml of LB Medium with antibiotic. For low copy plasmids, inoculate 100 - 200 ml of medium.</div></td></tr>
</table>Cjdghttp://2009.igem.org/wiki/index.php?title=Team:IPN-UNAM-Mexico/Protocols&diff=163569&oldid=prevCjdg at 01:28, 22 October 20092009-10-22T01:28:15Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__FORCETOC__</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__FORCETOC__</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{TOCright}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{TOCright}}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">=Protocols=</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== MIDIPREP ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== MIDIPREP ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Francisco Razo Hernández (noviembre 2008). Modified from “QIagen Plasmid Purification Midi”.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Francisco Razo Hernández (noviembre 2008). Modified from “QIagen Plasmid Purification Midi”.</div></td></tr>
</table>Cjdghttp://2009.igem.org/wiki/index.php?title=Team:IPN-UNAM-Mexico/Protocols&diff=139812&oldid=prevCjdg at 07:50, 21 October 20092009-10-21T07:50:22Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__FORCETOC__</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__FORCETOC__</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{TOCright}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{TOCright}}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">== MIDIPREP ==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Francisco Razo Hernández (noviembre 2008). Modified from “QIagen Plasmid Purification Midi”.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#Inoculate a colony in 25 - 50 ml of LB Medium with antibiotic. For low copy plasmids, inoculate 100 - 200 ml of medium.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#Centrifuge 20 minutes at 4000 rpm and get the pellet.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#Suspend the pellet in 4 ml of P1 QIagen Buffer (Be sure to add RNAse to the buffer).</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#Add 4 ml of P2 QIagen and mix on inversion 6 times.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#Add 4 ml of P3 QIagen and mix on inversion, add 10 ml of chloroform and mix on inversion 6 times.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#Incubate on ice 10 minutes and centrifugate 20 minutes at 4000 rpm.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#Recover the aqueous phase and add 5ml of isopropanol, incubate 30 minutes on ice.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#Centrifugate 15 minutes at 13000 rpm.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#Wash with EtOH at 70% and centrifugate 2 min at 13000 rpm, let the pellet dry and suspend on 500 ml of esterile deionized water (Water for pcr).</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== [[Image:Month-icon.png | 50px]] Competent cells with RbCl ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== [[Image:Month-icon.png | 50px]] Competent cells with RbCl ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Take 5 ml of liquid SOB and incubate at 37 °C overnight</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Take 5 ml of liquid SOB and incubate at 37 °C overnight</div></td></tr>
</table>Cjdghttp://2009.igem.org/wiki/index.php?title=Team:IPN-UNAM-Mexico/Protocols&diff=138539&oldid=prevLUIS DE JESUS at 05:21, 21 October 20092009-10-21T05:21:18Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__FORCETOC__</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__FORCETOC__</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{TOCright}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{TOCright}}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>== Competent cells with RbCl ==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>== <ins class="diffchange diffchange-inline">[[Image:Month-icon.png | 50px]] </ins>Competent cells with RbCl ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Take 5 ml of liquid SOB and incubate at 37 °C overnight</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Take 5 ml of liquid SOB and incubate at 37 °C overnight</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Take 1 ml of cell culture and innoculate into 500 ml of YENB media and incubate at 37 °C and 200 rpm until O.D.=0.5-0.55</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Take 1 ml of cell culture and innoculate into 500 ml of YENB media and incubate at 37 °C and 200 rpm until O.D.=0.5-0.55</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Adjust pH to 6.8 with 0.2 M of NaOH and sterilize by filtration using a 0.22 μm filter</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Adjust pH to 6.8 with 0.2 M of NaOH and sterilize by filtration using a 0.22 μm filter</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==CTAB miniprep==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==<ins class="diffchange diffchange-inline">[[Image:Month-icon.png | 50px]] </ins>CTAB miniprep==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Take 1.5 ml of cell culture and centrifuge at 15000 rpm for 3 minutes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Take 1.5 ml of cell culture and centrifuge at 15000 rpm for 3 minutes.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Discard liquid phase</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Discard liquid phase</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Incubate at 37°C for 30 min.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Incubate at 37°C for 30 min.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>== DNA purification from agarose gel ==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>== <ins class="diffchange diffchange-inline">[[Image:Month-icon.png | 50px]] </ins>DNA purification from agarose gel ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#In a UV room, cut the gel with a knife and place DNA bands inside falcon tubes</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#In a UV room, cut the gel with a knife and place DNA bands inside falcon tubes</div></td></tr>
</table>LUIS DE JESUShttp://2009.igem.org/wiki/index.php?title=Team:IPN-UNAM-Mexico/Protocols&diff=136307&oldid=prevJOSE at 01:24, 21 October 20092009-10-21T01:24:50Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Add 4 μl of lysozyme and mix with vortex.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Add 4 μl of lysozyme and mix with vortex.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Incubate for reaction to occur for 5 min at 37°C then boil for 45 seconds.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Incubate for reaction to occur for 5 min at 37°C then boil for 45 seconds.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#Boil 45 seconds inside a glass with <del class="diffchange diffchange-inline"><math> H2O </math></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#Boil 45 seconds inside a glass with <ins class="diffchange diffchange-inline">water</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Centrifuge at 15000 rpm for 10 min.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Centrifuge at 15000 rpm for 10 min.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Discard pellet.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Discard pellet.</div></td></tr>
</table>JOSEhttp://2009.igem.org/wiki/index.php?title=Team:IPN-UNAM-Mexico/Protocols&diff=136240&oldid=prevJOSE at 01:18, 21 October 20092009-10-21T01:18:34Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Add 4 μl of lysozyme and mix with vortex.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Add 4 μl of lysozyme and mix with vortex.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Incubate for reaction to occur for 5 min at 37°C then boil for 45 seconds.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Incubate for reaction to occur for 5 min at 37°C then boil for 45 seconds.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#Boil 45 seconds inside a glass with <math>H2O</math></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#Boil 45 seconds inside a glass with <math> H2O </math></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Centrifuge at 15000 rpm for 10 min.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Centrifuge at 15000 rpm for 10 min.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Discard pellet.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Discard pellet.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Discard supernatant.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Discard supernatant.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Wash with 1 ml of 70% ethanol and centrifuge for 3 min.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Wash with 1 ml of 70% ethanol and centrifuge for 3 min.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#<del class="diffchange diffchange-inline">Discard </del>ethanol by decantation.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#<ins class="diffchange diffchange-inline">Drop out </ins>ethanol by decantation.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#To dry remaining ethanol use a thermoblock at 65°C.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#To dry remaining ethanol use a thermoblock at 65°C.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Resuspend with 100 μl of sterile water and add 1 μl of RNase.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Resuspend with 100 μl of sterile water and add 1 μl of RNase.</div></td></tr>
</table>JOSEhttp://2009.igem.org/wiki/index.php?title=Team:IPN-UNAM-Mexico/Protocols&diff=135614&oldid=prevCjdg at 00:12, 21 October 20092009-10-21T00:12:38Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:IPN-UNAM-Mexico}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:IPN-UNAM-Mexico}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__FORCETOC__</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__FORCETOC__</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">{{TOCright}}</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Competent cells with RbCl ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Competent cells with RbCl ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Take 5 ml of liquid SOB and incubate at 37 °C overnight</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Take 5 ml of liquid SOB and incubate at 37 °C overnight</div></td></tr>
</table>Cjdghttp://2009.igem.org/wiki/index.php?title=Team:IPN-UNAM-Mexico/Protocols&diff=135315&oldid=prevJOSE at 23:28, 20 October 20092009-10-20T23:28:35Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 23:28, 20 October 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Repeat 1 and 2</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Repeat 1 and 2</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Resuspend cells with 1 ml of NaCl 1.2 M with vortex</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Resuspend cells with 1 ml of NaCl 1.2 M with vortex</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#Centrifuge at <del class="diffchange diffchange-inline">13500 </del>rpm for 3 minutes and discard supernatant.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#Centrifuge at <ins class="diffchange diffchange-inline">15000 </ins>rpm for 3 minutes and discard supernatant.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Add 100 μl of sterile water and resuspend with vortex</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Add 100 μl of sterile water and resuspend with vortex</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Add 200 μl of STET and mix with vortex.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Add 200 μl of STET and mix with vortex.</div></td></tr>
</table>JOSE