Team:Illinois/Protocols
From 2009.igem.org
(→Standard) |
(→Standard) |
||
Line 30: | Line 30: | ||
*[[BioBrick information]] | *[[BioBrick information]] | ||
- | [[Preparing Cryo stocks]] | + | *[[Preparing Cryo stocks]] |
<html> | <html> | ||
</div> | </div> |
Revision as of 20:58, 19 July 2009
Protocols
This page describes protocols or includes links to protocols used in our project. Recipes used are also listed in a separate section. Protocols are organized by category. They were collected from Open Wet Ware, our advisors, and labs around the University of Illinois campus.
Standard
sRNA Characterization
Taken from: A Green Fluorescent Protein (GFP)-Based Plasmid System to Study Post-Transcriptional Control of Gene Expression In Vivo: Johannes H. Urban and Jörg Vogel, 2009
This protocol is used to characterize an E. coli small RNA and its ability to downregulate a reporter gene (GFP). The small RNA is cloned out of the E. coli chromosome and inserted into a high-copy number plasmid (pJU-334). The sRNA's target sequence is also cloned out of the E. coli chromosome and inserted into a low-copy number plasmid (pXG-10). Both plasmids are then transformed into E. coli Top10F' cells. Successful sRNA repression is indicated by a decreased level of fluorescence in the cells.
Questions about our Wiki page? Please email us at illinoisiGEM@gmail.com.