Team:Illinois/Protocols
From 2009.igem.org
Contents |
Protocols
This page describes protocols or includes links to protocols used in our project. Recipes used are also listed in a separate section. Protocols are organized by category.
Standard
sRNA Characterization
Taken from: A Green Fluorescent Protein (GFP)-Based Plasmid System to Study Post-Transcriptional Control of Gene Expression In Vivo: Johannes H. Urban and Jörg Vogel, 2009
This protocol is used to characterize an E. coli small RNA and its ability to downregulate a reporter gene (GFP). The small RNA is cloned out of the E. coli chromosome and inserted into a high-copy number plasmid (pJU-334). The sRNA's target sequence is also cloned out of the E. coli chromosome and inserted into a low-copy number plasmid (pXG-10). Both plasmids are then transformed into E. coli Top10F' cells. Successful sRNA repression is indicated by a decreased level of fluorescence in the cells.
sRNA characterization procedure
Recipes
Suggested Reading
Current Protocols in Molecular Biology
- A really good resource for explaining protocols and explaining the importance of the steps.
- Free online
- Recommended by Dr. Golding
Introduction to Systems Biology by Uri Alon
- A very good/famous introduction into the quantitative aspects of biology.
- Recommended by Dr. Golding
Questions about our Wiki page? Please email Dave Korenchan at korench1@illinois.edu.