Team:KULeuven/17 August 2009

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Project progress

Progress of parts

[edit] Blue Light Receptor

Liquid cultures have been made for the kanamycine resistant plasmid backbone Status: 500 Content-type: text/html

Software error:

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For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

and for the LigA (BLP+Status: 500

Content-type: text/html

Software error:

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For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

in Status: 500

Content-type: text/html

Software error:

Can't locate Bio/SeqIO.pm in @INC (@INC contains: /synbio/lib /web-site/parts.igem.org/cgi/lib /usr/local/lib64/perl5 /usr/local/share/perl5 /usr/lib64/perl5/vendor_perl /usr/share/perl5/vendor_perl /usr/lib64/perl5 /usr/share/perl5 .) at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
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For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

).

Lig B has been re-ented on a new plate because too many cells had grown on the one we made on aug 14. We always used single kolonies to re-ent.

[edit] Vanillin Production

  • SAMS (ligation sam5+sam8) and EF (ligation ech+fcs): electroporated ...
  • and plated out

[edit] Vanillin Receptor

  • A failed again so we decided to work only with W instead of performing a new PCR. TOPO for W and Z worked because there were colonies visible. White colonies were plated out.
  • Fluid cultures of G2 and G3 were miniprepped, nanodropped and cut with Dpn I to exclude the motherstrain
  • Mutagenesis 2 on R on position 427 was performed with PCR

[edit] Key/Lock/Anti-Key