Team:KULeuven/17 August 2009
Revision as of 08:48, 10 September 2009 by Deepstar
Progress of parts
 Blue Light Receptor
Liquid cultures have been made for the kanamycine resistant plasmid backbone pSB3K3 and for the LigA (BLP+BBa_E0240 in pSB1A2).
Lig B has been re-ented on a new plate because too many cells had grown on the one we made on aug 14. We always used single kolonies to re-ent.
 Vanillin Production
- SAMS (ligation sam5+sam8) and EF (ligation ech+fcs): electroporated ...
- and plated out
 Vanillin Receptor
- A failed again so we decided to work only with W instead of performing a new PCR. TOPO for W and Z worked because there were colonies visible. White colonies were plated out.
- Fluid cultures of G2 and G3 were miniprepped, nanodropped and cut with Dpn I to exclude the motherstrain
- Mutagenesis 2 on R on position 427 was performed with PCR