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Team:Kyoto/CiC/Experiment
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[[Image:sigGFP.png|700px|thumb|center|]] | [[Image:sigGFP.png|700px|thumb|center|]] | ||
†RBS+Signal for TIM23 complex::GFP+terminator was made by two-stage PCR. | †RBS+Signal for TIM23 complex::GFP+terminator was made by two-stage PCR. | ||
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First-stage PCR | First-stage PCR | ||
Forward primer; | Forward primer; | ||
Revision as of 05:21, 20 October 2009
Experiment
Construction
HIV-TAT::(LALAAAA)3 expressing vector
†RBS+HIV-TAT+HIStag+(LALAAAA)3 was made by elongation of primer dimer. (Forward primer; cggaattcgcggccgcttctagagaaagaggagaaatactagATGTATGGACGTAAAAAACGTCGTGGACGTCGTCGTGGCGGCGGTCATCATCATCATCACCATGGCGG Reverse primer; ctgcagcggccgctactagtaTTACGCGGCCGCCGCCAGGGCCAGCGCGGCCGCCGCCAGGGCCAGCGCGGCCGCCGCCAGGGCCAGGCCACCGCCATGGTGATGATGATG)
Signal for TIM23 complex::GFP expressing vector
†RBS+Signal for TIM23 complex::GFP+terminator was made by two-stage PCR.
First-stage PCR Forward primer; TTTAAACCGGCGACCCGTACCCTGTGCTCTTCTCGTTATCTGCTGcgtaaaggagaagaacttttcactggagttg Reverse primer; agtgagctgataccgctcgc
Second-stage PCR Forward primer; cggaattcgcggccgcttctagagaaagaggagaaatactagATGCTGAGCCTGCGTCAGTCTATTCGTTTTTTTAAACCGGCGACCCGTAC Reverse primer; agtgagctgataccgctcgc
