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Team:Kyoto/CiC/Experiment
From 2009.igem.org
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===Construction=== | ===Construction=== | ||
HIV-TAT::(LALAAAA)3 expressing vector | HIV-TAT::(LALAAAA)3 expressing vector | ||
| - | [[Image: | + | [[Image:kyoto_HIV-TAT.png]] |
†RBS+HIV-TAT+HIStag+(LALAAAA)3 was made by elongation of primer dimer. | †RBS+HIV-TAT+HIStag+(LALAAAA)3 was made by elongation of primer dimer. | ||
(Forward primer; | (Forward primer; | ||
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Signal for TIM23 complex::GFP expressing vector | Signal for TIM23 complex::GFP expressing vector | ||
| - | [[Image: | + | [[Image:kyoto_sigGFP.png]] |
†RBS+Signal for TIM23 complex::GFP+terminator was made by two-stage PCR. | †RBS+Signal for TIM23 complex::GFP+terminator was made by two-stage PCR. | ||
Revision as of 05:27, 20 October 2009
Experiment
Construction
HIV-TAT::(LALAAAA)3 expressing vector
†RBS+HIV-TAT+HIStag+(LALAAAA)3 was made by elongation of primer dimer.
(Forward primer;
cggaattcgcggccgcttctagagaaagaggagaaatactagATGTATGGACGTAAAAAACGTCGTGGACGTCGTCGTGGCGGCGGTCATCATCATCATCACCATGGCGG Reverse primer;
ctgcagcggccgctactagtaTTACGCGGCCGCCGCCAGGGCCAGCGCGGCCGCCGCCAGGGCCAGCGCGGCCGCCGCCAGGGCCAGGCCACCGCCATGGTGATGATGATG)
Signal for TIM23 complex::GFP expressing vector
†RBS+Signal for TIM23 complex::GFP+terminator was made by two-stage PCR.
First-stage PCR Forward primer;
TTTAAACCGGCGACCCGTACCCTGTGCTCTTCTCGTTATCTGCTGcgtaaaggagaagaacttttcactggagttg
Reverse primer;
agtgagctgataccgctcgc
Second-stage PCR Forward primer;
cggaattcgcggccgcttctagagaaagaggagaaatactagATGCTGAGCCTGCGTCAGTCTATTCGTTTTTTTAAACCGGCGACCCGTAC
Reverse primer;
agtgagctgataccgctcgc
