Team:Kyoto/CiC/Notebook/1019-1023

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0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

1019-1025 :Obserbation


Monday, 19 October

To Do

  • subgoal A --- Confirmation of function of TAT-liposome
    • observation by microscope
  • Subgoal B --- Liposomes with Mitochondrial Translocases
    • Mitochondoria isolation
    • expressing sig-GFP in vitro with mitochondoria
  • Subgoal C --- sig-EGFP expression in HeLa
    • transfomation

Results

subgoal A --- Confirmation of function of TAT-liposome

Liposome was taken by HeLa cells.See the results.

Subgoal C --- sig-EGFP expression in HeLa

sig-EGFP was made a localization in mitochondoria. See the results.



Tuesday, 20 October

To Do

  • Subgoal B --- Liposomes with Mitochondrial Translocases
    • observation with microscope

Results

See the results.



Wednesday, 21 October

To Do

  • subgoal A --- Confirmation of function of TAT-liposome
    • making liposome by natural swelling method and extruder
    • add liposome to HeLa cells

Results

According to the expectation,HIV-TAT-(LALAAAA)3-generator may work. Liposome is taken by HeLa Cells. See the results.